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pRARE-MBP-DEST

Plasmid

#84650

Purpose

Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

Depositor

Neil Olszewski

Article

Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

Type

Empty backbone

Tags

MBP

Expression

Bacterial

Promoter

tac

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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SFG.CNb30_opt.IRES.eGFP

Plasmid

#22493

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

Codon optimised Calcineurin B mutant 30 (PPP3R1 Human)

Use

Retroviral

Mutation

L124T; K125-LA-Ins

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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DJ-1 TEV site pET15b

Plasmid

#60687

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

TEV site was inserted (see comments below).

Promoter

T7

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pUC19.TRIM28.FKBP.knock-in.mRuby2

Plasmid

#185008

Purpose

For knocking in FKBP F36V P2A mRuby2

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

FKBP F36V P2A mRuby2

Use

Cloning

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19.TRIM28.FKBP.knock-in.mCerulean

Plasmid

#185009

Purpose

For knocking in FKBP F36V P2A mCerulean

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

FKBP F36V P2A mCerulean

Use

Cloning

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLPCX cyto Grx1-roGFP2

Plasmid

#64975

Purpose

Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pEB3-mBaoJin

Plasmid

#229565

Purpose

Expresses EB3-mBaoJin fusion in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Expression

Mammalian

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-NUP98

Plasmid

#229593

Purpose

Expresses EGFP-NUP98 fusion in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

EGFP

Expression

Mammalian

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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pEIGW Grx1-roGFP2

Plasmid

#64990

Purpose

Mammalian expression of cytosolic Grx1-roGFP2 (lentiviral vector)

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Lentiviral

Tags

roGFP2

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pBADHisB-Sumo-mBaoJin

Plasmid

#229580

Purpose

Expresses mBaoJin in bacterial cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Expression

Bacterial

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Puro_siKD

Plasmid

#86695

Purpose

Tetracycline inducible expression of shRNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Expression

Mammalian

Promoter

H1 TO

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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SFG.wtCNb_opt.IRES.eGFP

Plasmid

#22492

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

Codon optimised wild type Calcineurin B (PPP3R1 Human)

Use

Retroviral

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pmBaoJin-Actin

Plasmid

#229609

Purpose

Expresses mBaoJin-actin fusion in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Expression

Mammalian

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-lifeactin

Plasmid

#229607

Purpose

Expresses mNeonGreen-lifeactin fusion in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mNeonGreen

Expression

Mammalian

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCXN2-BCL6 (CXN-BCL6)

Plasmid

#40346

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

BCL6 (BCL6 Human)

Tags

FLAG

Expression

Mammalian

Promoter

pCAG

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pBabePuro-ApoECDS

Plasmid

#42949

Depositor

Sohail Tavazoie

Article

Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

Insert

Apolipoprotein E (APOE Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only
pMX_mTBP

Plasmid

#44333

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TBP (Tbp Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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CDK1as_T2A_puromycin

Plasmid

#118595

Purpose

One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

Depositor

William Earnshaw

Article

Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

Insert

CDK1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLC242-GFP-metap2

Plasmid

#100518

Purpose

Expresses GFP tagged metap2 in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

Metap2 (METAP2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-CreERT2 stuffer v3

Plasmid

#158046

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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