We narrowed to 18,568 results for: REV

1. 12URA-B-ScTop2∆CTD

   Plasmid

   #214924

   Purpose

   Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

   Depositor

   James Berger

   Article

   Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

   Insert

   topoisomerase II
   

   Tags

   6x His-TEV

   Expression

   Yeast

   Mutation

   Deleted amino acids 1178-1428

   Promoter

   Gal 1/10

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-hBcan

   Plasmid

   #18966

   Depositor

   Mariano Viapiano

   Article

   Hu et al J Biol Chem. 2008 Sep 5;283(36):24848-59

   Insert

   Brevican (BCAN Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pRG659_lexO-FseI_LexA-TF_LEU2MX

   Plasmid

   #154816

   Purpose

   Inducible expression of FseI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   FseI endonuclease
   LexA-ER-B112
   

   Tags

   FLAG and NLS

   Expression

   Yeast

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PhyB-mCherry-Tom7

   Plasmid

   #66571

   Purpose

   Constitutively express mitochondrial localized PhyB in Saccharomyces cerevisiae

   Depositor

   Orion Weiner

   Article

   Jost et al ACS Synth Biol. 2015 Jun 2.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Tags

   Tom7 and mCherry

   Expression

   Yeast

   Mutation

   PhyB portion contains aa's 1-908

   Promoter

   pADH1

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRG714_lexO-AscI_LexA-TF_URA3MX

   Plasmid

   #154822

   Purpose

   Inducible expression of AscI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AscI endonuclease
   LexA-ER-B112
   

   Tags

   FLAG and NLS

   Expression

   Yeast

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRG645_lexO-AscI_LexA-TF_LEU2MX

   Plasmid

   #154813

   Purpose

   Inducible expression of AscI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AscI endonuclease
   LexA-ER-B112
   

   Tags

   FLAG and NLS

   Expression

   Yeast

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET151-Vps4

   Plasmid

   #87733

   Purpose

   Bacterial expression of full-length S. cerevisiae Vps4

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDDGFP-Leu2d-ScVrg4

   Plasmid

   #102334

   Purpose

   Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

   Insert

   YGL225W (VRG4 Budding Yeast)
   

   Tags

   GFP

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICH47732:PtFCP:ShBle

   Plasmid

   #104893

   Purpose

   Expresses ShBle with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation.

   Depositor

   Thomas Mock

   Article

   Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

   Insert

   ShBle
   

   Use

   Phaeodactylum; golden gate assembly

   Tags

   PtFCP promoter and terminator

   Available Since

   Dec. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-RaACR_687-EYFP

    Plasmid

    #111077

    Purpose

    Anion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RaACR_687
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. cPE

    Plasmid

    #183549

    Purpose

    Expresses compact Prime Editor in mammalian cells

    Depositor

    Wen Xue

    Article

    Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

    Insert

    SpCas9 nickase (H840A), Reverse Transcriptase
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHES609 [prNOp7-PBAC]

    Plasmid

    #103017

    Purpose

    A S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.

    Depositor

    Hana El-Samad

    Article

    Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

    Insert

    bPAC
    

    Expression

    Yeast

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Let7R-SunTag

    Plasmid

    #196929

    Purpose

    Reverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.

    Depositor

    Jeffrey Chao

    Article

    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

    Insert

    Renilla luciferase
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pILGFPQ4

    Plasmid

    #83559

    Purpose

    Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    GAL7 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    GAL7

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Dom34(full length)

    Plasmid

    #19098

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

16. iRFP713-FRB-Mss4-Kina

    Plasmid

    #202744

    Purpose

    expresses recruitable fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    iRFP713:MTOR(2021-2113):GGSA2:S. cerevisiae Mss4 (377-756) (MSS4 Budding Yeast)
    

    Expression

    Mammalian

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Let7R-IRE-TREAT

    Plasmid

    #196934

    Purpose

    Reverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT.

    Depositor

    Jeffrey Chao

    Article

    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

    Insert

    Renilla luciferase
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYES2.1-cerMET5

    Plasmid

    #107437

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET5 (MET5 Budding Yeast)
    

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    V81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …

    Promoter

    GAL1

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAGM4723-Del

    Plasmid

    #112207

    Purpose

    Level 2 cloning vector, for golden gate cloning for diatoms. All the Agrobacterium T-DNA delivery were deleted.

    Depositor

    Thomas Mock

    Article

    Graff van Creveld et al Elife. 2023 Jun 6;12:e84392. doi: 10.755

    Type

    Empty backbone

    Use

    Golden gate assembly

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. JBEI-6833

    Plasmid

    #84983

    Purpose

    Expression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.

    Depositor

    Taek Soon Lee

    Article

    George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

    Insert

    NudB-PMD
    

    Expression

    Bacterial

    Promoter

    trc promoter

    Available Since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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