We narrowed to 5,092 results for: codon optimized

1. HotPETase_pET21b

   Plasmid

   #215407

   Purpose

   Expression construct for HotPETase gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   HotPETase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-TetOne-Puro-EcSTH-FLAG

   Plasmid

   #218628

   Purpose

   This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   EcSTH
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-N1-IL6

   Plasmid

   #111933

   Purpose

   Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

   Insert

   Interleukin 6 (IL6 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells

   Promoter

   CMV

   Available since

   July 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAIP hIL4 co

   Plasmid

   #74169

   Purpose

   Lentiviral vector for expression of human codon optimized IL4 with puromycin resistance.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   IL4 (IL4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   June 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac Dual α1B/β3 Tubulin

   Plasmid

   #170552

   Purpose

   Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

   Depositor

   Tarun Kapoor

   Article

   Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

   Inserts

   TUBA1B (TUBA1B Human)
   TUBB3 (TUBB3 Human)
   

   Use

   Tags

   AP linker, GS linker (GGSGG), His10 tag, L21 enha…

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CoV2-N-R203M

   Plasmid

   #177952

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (R203M) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R203M

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-TetOne-Puro-mitoEcSTH-FLAG

   Plasmid

   #218629

   Purpose

   This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   mitoEcSTH
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG and MTS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGGB_YFP-MiniTurboID

   Plasmid

   #222433

   Purpose

   Golden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.

   Depositor

   Dominique Bergmann

   Article

   Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

   Insert

   MiniTurboID (BirA mutant)
   

   Use

   Golden gate / green gate compatible cloning vector

   Tags

   Linker and YFP

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

   Plasmid

   #42229

   Purpose

   This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. GB1-A11(2-196)-Strep

    Plasmid

    #196208

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Use

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

    Plasmid

    #104563

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1 (D832A)
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpCas9-ABE8e(V106W)

    Plasmid

    #198547

    Purpose

    Expresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-ABE8e(V106W)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. GB1-A11(2-88)-Strep

    Plasmid

    #196214

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-88)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Use

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. eSpCas9(1.1)

    Plasmid

    #138555

    Purpose

    Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221_SLC39A10

    Plasmid

    #132275

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

    Insert

    SLC39A10 (SLC39A10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. SpCas9-NG-YE1-BE4max

    Plasmid

    #198535

    Purpose

    Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHSN401

    Plasmid

    #50588

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    maize codon optimized

    Promoter

    2×35Sp and AtU6-26p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSP1673

    Plasmid

    #65769

    Purpose

    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-FLAG-Sestrin2

    Plasmid

    #72595

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN2 (SESN2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42337

    Purpose

    Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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