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163,331 results

Showing: 6601 - 6640 of 163331 results

  1. pBA950

    Plasmid
    #122239
    Purpose
    CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122239-plasmid-map-sequence-id-292012

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  2. pOpen-ETSSB

    Plasmid
    #165549
    Purpose
    ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    Extreme Thermostable Single-Stranded DNA Binding Protein
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165549-plasmid-map-sequence-id-339430

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  5. pVP143

    Plasmid
    #222080
    Purpose
    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and AeBlue for plasmid eviction.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1
    Insert
    dCas9-PmCDA1, sfGFP, sacB and AeBlue
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    222080-plasmid-map-sequence-id-438352

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  12. pmal_c5x_RNAse_Inhib

    Plasmid
    #153314
    Purpose
    Bacterial expression of an RNase inhibitor
    Depositor
    Philippa Marrack, Drew Endy
    Insert
    RNAse Inhibitor
    Use
    Tags
    Maltose Binding Protein
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153314-plasmid-map-sequence-id-298771

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  25. pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50454
    Purpose
    Double floxed Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV8
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50454-plasmid-map-sequence-id-214300

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  26. TALE Toolbox

    Plasmid Kit
    #1000000019
    Purpose
    Provides a complete set of plasmids for building your own TALE nucleases (TALENs) and transcription factors (TALE-TFs) for genome editing and transcription modulation applications.
    Depositor
    Feng Zhang
    Available since
    Dec. 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  32. TFORF0172

    Plasmid
    #142551
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ONECUT2 (ONECUT2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    142551-plasmid-map-sequence-id-397438

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  33. pAAV-CaMKIIa-EGFP (AAV Retrograde)

    Viral Prep
    #50469-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-CaMKIIa-EGFP (#50469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-EGFP plasmid DNA. CamKIIa-driven EGFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    CaMKII
    Tags
    EGFP
    Available since
    Sept. 13, 2021
    Availability
    Academic Institutions and Nonprofits only

  35. pDIRECT_22C

    Plasmid
    #91135
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91135-plasmid-map-sequence-id-182261

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  40. pMKO.1 GFP

    Plasmid
    #10676
    Purpose
    Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.
    Depositor
    William Hahn
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    10676-plasmid-map-sequence-id-139182

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Showing: 6601 - 6640 of 163331 results