We narrowed to 9,799 results for: otos

1. SMC2 CRISPR

   Plasmid

   #140649

   Purpose

   SMC2 tagging CRISPR

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   SMC2 (SMC2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNAIIIB rhoA QL

   Plasmid

   #61632

   Purpose

   mammalian expression of rhoA constitutively active mutant

   Depositor

   JS Gutkind

   Article

   Coso et al Cell. 1995 Jun 30;81(7):1137-46.

   Insert

   rhoA (RHOA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q63L, constitutively active

   Promoter

   CMV

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pETM6-RgTALsyn-HpaBC

   Plasmid

   #100949

   Purpose

   Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Inserts

   RgTALsyn
   HpaB
   HpaC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   T7

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_Flex_syn_SpikeyGi2_kv2.1

   Plasmid

   #195314

   Purpose

   Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, double-flowed in AAV vector under the promoter hSyn

   Depositor

   Vincent Pieribone

   Article

   Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

   Insert

   SpikeyGi2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin promoter

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUB-nSypHer (NLS:sypHer:NLS)

   Plasmid

   #84731

   Purpose

   Expresses sypHer in plant cells-biosensor for pH targeted to Nuclei

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Insert

   nSypHer
   

   Use

   Tags

   NLS (nuclear localisation signal and NLS (nuclear…

   Expression

   Plant

   Mutation

   Promoter

   ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-DIO-mCherry-WPRE

   Plasmid

   #202620

   Purpose

   Double floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a)

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   mCherry
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-P1-LoxP-Neo-N-miRFP670-C

   Plasmid

   #159434

   Purpose

   CRISPR knock-in donor construct with fluorescent reporter and removable selectable marker

   Depositor

   Jianhua Xing

   Article

   Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

   Inserts

   Neomycin
   miRFP670
   

   Use

   CRISPR

   Tags

   3' linker pair 1, 5' linker pair 1, C t…

   Expression

   Mutation

   Promoter

   SV40

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28b_RanT24N

   Plasmid

   #186271

   Purpose

   Expression vector of His-RanT24N in E.coli

   Depositor

   Masahito Ikawa

   Article

   Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   GTP-binding nuclear protein Ran (Ran Mouse)
   

   Use

   Tags

   His tagged and His-tagged at N terminal

   Expression

   Bacterial

   Mutation

   Changed T24 into N

   Promoter

   T7 promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCerulean-LaminB1-10

   Plasmid

   #55380

   Purpose

   Localization: Nuclear Envelope, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   LaminB1 (LMNB1 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pDEST- IP2.0(codon for C.elegans)

    Plasmid

    #109216

    Purpose

    IP2.0 for C. elegans (Codon optimization and introns).

    Depositor

    Takeshi Ishihara

    Article

    Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

    Insert

    IP2.0 (Inverse Pericam 2.0)
    

    Use

    Tags

    His-tag

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-Rab21-CCSS

    Plasmid

    #83424

    Purpose

    Mouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.

    Depositor

    Johanna Ivaska

    Article

    Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

    Insert

    Rab21 (Rab21 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Residues 218 and 219 from C to S (CCSS: TGCTGT -&…

    Promoter

    CMV

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCerulean3-Mito-7

    Plasmid

    #55433

    Purpose

    Localization: Mitochondria, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pcDNAIIIB HA JNK

    Plasmid

    #61630

    Purpose

    mammalian expression of JNK

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    JNK1 beta1 isoform (MAPK8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

14. mCerulean3-Golgi-7

    Plasmid

    #55420

    Purpose

    Localization: GalT Targeting Sequence, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

15. mTagRFP-T-Actin-7

    Plasmid

    #58001

    Purpose

    Localization: Actin, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pECE-M2-PP1Cbeta

    Plasmid

    #31677

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    protein phosphatase 1, catalytic subunit, beta isozyme (PPP1CB Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pRSET-pr-mEos3.1 (mEosFP3.1-A69T)

    Plasmid

    #99221

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos3.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A69T for primed conversion applications

    Promoter

    T7

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

    Plasmid

    #79614

    Purpose

    Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Inserts

    SpCas9
    PmCDA1
    

    Use

    Tags

    SH3 domain and SHL

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1 and pGal10

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCerulean3-Fibrillarin-7

    Plasmid

    #55419

    Purpose

    Localization: Nucleoli, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

20. mKO-Mito-7

    Plasmid

    #57848

    Purpose

    Localization: Mitochondria, Excitation: 548, Emission: 561

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mKO

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. px335-sgRNA-EF(Cas9n)-Dnmt3b-R

    Plasmid

    #122333

    Purpose

    Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Dnmt3b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJH4504

    Plasmid

    #191702

    Purpose

    Pnmr-1 long::TeTx::wCherry unc-54 3' UTR C.elegans AVA/other neurons expression of TeTx RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    Tetanus Toxin Light Chain
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pnmr-1 long

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. CAG::Gdnf

    Plasmid

    #78209

    Purpose

    piggybac vector for mouse Gdnf expression

    Depositor

    Ivo Lieberam

    Article

    Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

    Insert

    Gdnf (Gdnf Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

24. eSpCas9-EF-Gsk3b(new)

    Plasmid

    #122341

    Purpose

    Expresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Gsk3b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MSCV-PIG-N3FLAG-TAF10

    Plasmid

    #105990

    Purpose

    retrovirally express mouse TAF10 with 3*FLAG tag at N terminal, GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    FLAG tagged TAF10 (Taf10 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-TRBP-L1+D1+L2-myc/His

    Plasmid

    #180599

    Purpose

    Expressing various parts of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCI-neo-mApg5

    Plasmid

    #22956

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

    Insert

    autophagy related genes 5 (Atg5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

28. mCherry-MyosinIIC-N-18

    Plasmid

    #55108

    Purpose

    Localization: Cytoskeleton, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

    Insert

    MyosinIIC
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pMRX-IP-EGFP-mouse Plaat3 (N157stop)

    Plasmid

    #168771

    Purpose

    Expresses EGFP-Plaat3 (C-terminal element is deleted) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

    Insert

    Hrasls3 (Plaat3 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pECE-M2-PAK2 S20A

    Plasmid

    #31681

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    p21-activated protein kinase 2 S20A (PAK2 Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    changed Serine 20 to Alanine

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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