Skip to main content
Addgene
Showing: 3341 - 3360 of 160434 results

  2. pGW1-mCherry-EGFP-PIM

    Plasmid
    #111759
    Purpose
    High expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition
    Depositor
    Lukas Kapitein
    Article
    Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:
    Insert
    2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …
    Promoter
    CMV
    Available since
    Oct. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111759-plasmid-map-sequence-id-222507

    Enlarge

  6. qTAG-C-Solo-mStayGold-LAMP1

    Plasmid
    #227322
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LAMP1 Homology Arms flanking a mStayGold Tag (LAMP1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227322-plasmid-map-sequence-id-452101

    Enlarge

  11. pMZ374

    Plasmid
    #59896
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).
    Depositor
    Mikel Zaratiegui
    Article
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59896-plasmid-map-sequence-id-313658

    Enlarge

  18. pRDA_526

    Plasmid
    #184859
    Purpose
    Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.
    Insert
    sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    184859-plasmid-map-sequence-id-362663

    Enlarge

  19. pAAV-hSyn-hM4D(Gi)-mCherry (AAV Retrograde)

    Viral Prep
    #50475-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-hM4D(Gi)-mCherry (#50475). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM4D(Gi)-mCherry plasmid DNA. CNO-induced neuronal silencing. Synapsin-driven with mCherry reporter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry
    Available since
    Aug. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 3341 - 3360 of 160434 results