166,818 results

1. pAAV-Ef1a-fDIO mCherry (AAV5)

   Viral Prep

   #114471-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   EF1a

   Tags

   mCherry (Flp-dependent)

   Available Since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

2. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #180280

   Purpose

   The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (backbone) and BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLH01

   Plasmid

   #117261

   Purpose

   Genome editing for Lactobacillus plantarum WCSF1

   Depositor

   Sheng Yang

   Article

   Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

   Insert

   PorfX-Lp_0640-42, sapK-sappR
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PIEZO1-HaloTag

   Plasmid

   #207834

   Purpose

   PIEZO1 fusion for biochemistry and live imaging

   Depositor

   Charles Cox

   Article

   Zhou et al Science. 2023 Aug 18;381(6659):799-804.

   Insert

   PIEZO1 (PIEZO1 Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCTcon2

   Plasmid

   #41843

   Depositor

   Dane Wittrup

   Article

   Chao et al Nat Protoc. 2006;1(2):755-68.

   Insert

   CD20
   

   Use

   Tags

   HA and c-myc

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO eNpHR 3.0-EYFP (AAV5)

   Viral Prep

   #26966-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO eNpHR 3.0-EYFP (#26966). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO eNpHR 3.0-EYFP plasmid DNA. EF1a-driven, cre-dependent eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

   Promoter

   EF1a

   Tags

   EYFP (Cre-dependent)

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pUK21

   Plasmid

   #49788

   Purpose

   E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

   Depositor

   Joachim Messing

   Article

   Vieira et al Gene. 1991 Apr;100:189-94.

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   lacZ

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. STICR_Index1

   Pooled Library

   #186334

   Purpose

   High Complexity Lentiviral Barcode Library

   Depositor

   Tomasz Nowakowski

   Article

   Delgado et al Nature. 2021 Dec 15. pii: 10.1038/s41586

   Expression

   Mammalian

   Species

   Synthetic

   Use

   Lentiviral

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

9. pSIRV-NF-kB-eCFP

   Plasmid

   #118094

   Purpose

   Flourescent reporter for NF-kB activity

   Depositor

   Peter Steinberger

   Article

   Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

   Insert

   eCFP
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   minimal promoter with NF-kB responsive element

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter

    Plasmid

    #61762

    Purpose

    Dual Color betaGlobin reporter to study splicing kinetics in vivo

    Depositor

    Daniel Larson

    Article

    Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

    Insert

    b-Globin
    

    Use

    Tags

    24 X MS2 hairpin cassette in 3'UTR of b-Glob…

    Expression

    Mammalian

    Mutation

    Promoter

    Dox inducible Tet-ON promoter

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pgRNA-bacteria

    Plasmid

    #44251

    Purpose

    Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    guide RNA (bacteria)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available Since

    April 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. hNav1.8_pcDNA3.1

    Plasmid

    #182847

    Purpose

    Expresses hNav1.8 in mammalian cells

    Depositor

    Paul DeCaen

    Article

    McCollum et al Cell Rep. 2022 Aug 23;40(8):111248. doi:

    Insert

    SCN10A (SCN10A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

    Plasmid

    #162378

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIS1

    Plasmid

    #12179

    Depositor

    David Bartel

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-n1-APP

    Plasmid

    #69924

    Purpose

    mammalian expression of human APP fused to EGFP

    Depositor

    Thomas Wassmer, Zita Balklava

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Insert

    APP (APP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-CAAX

    Plasmid

    #86056

    Purpose

    A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Homo sapiens Harvey rat sarcoma (HRAS Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains the region from 825-857 of HRAS as a far…

    Promoter

    CMV

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. makeTCR_Lentiviral_Backbone

    Plasmid

    #233943

    Purpose

    backbone for makeTCR assembly

    Depositor

    Michael Platten

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a (long)

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. DYRK1A/pTRE3G-FL-hXIST

    Plasmid

    #149608

    Purpose

    Express doxycycline-inducible full length of human XIST cDNA

    Depositor

    Jeanne Lawrence

    Article

    Jiang et al Nature. 2013 Aug 15;500(7462):296-300. d

    Insert

    X-inactive specific transcript (XIST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE3G

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRBC-sfGFP-150TAG

    Plasmid

    #174076

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Use

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    T7

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Nuclear Pro-Code Vector Kit

    Plasmid Kit

    #1000000197

    Purpose

    The Nuclear Pro-Code Vector Kit is a set of 75 lentiviral vector plasmids, each with a unique barcode (Pro-Code) and gRNA cloning site.

    Depositor

    Brian Brown

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only