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RNA-binding protein pooled CRISPR knockout library

Pooled library

#141438

Purpose

Knockout library targeting RNA-binding proteins with 10 guide RNAs per target.

Depositor

Eugene Yeo

Publication

Wheeler et al Nat Methods. 2020 May 11. pii: 10.1038/s

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
rAAV2/MG1.1

Plasmid

#184540

Purpose

AAV packaging plasmid, expressing Rep2 and AAV-MG1.1 capsid

Depositor

Minmin Luo

Publication

Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

Insert

AAV-MG1.1 capsid

Use

AAV; Helper vector for packaging

Mutation

changes on AAV9 capsid: K449R, A587L, Q588M; inse…

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pClneoMyc human ERBIN

Plasmid

#40214

Depositor

Yutaka Hata

Publication

Ohno et al Oncogene. 2002 Oct 10;21(46):7042-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ERBIN (ERBIN Human)

Tags

myc

Expression

Mammalian

Mutation

L143S, S274L and P338L mutation in ERBIN compared…

Promoter

CMV

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS HSPA8

Plasmid

#19541

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 8 (HSPA8 Human)

Tags

His

Expression

Mammalian

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3.3_SARS2_XBB

Plasmid

#194494

Purpose

expressing SARS-CoV-2 XBB spike protein for pseudovirus production

Depositor

David Nemazee

Publication

Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

Insert

Spike (XBB) (S SARS-CoV-2)

Expression

Mammalian

Mutation

V83A, Y144-,H146Q, Q183E, V213E, G339H, L368I, R3…

Promoter

CMV

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA5/FRT/TO V5 HSPA1A

Plasmid

#19510

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 1A (HSPA1A Human)

Tags

V5

Expression

Mammalian

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA5/FRT/TO V5 HSPA8

Plasmid

#19514

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 8 (HSPA8 Human)

Tags

V5

Expression

Mammalian

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pAAV-hSyn-GRAB-gDA3m (AAV1)

Viral prep

#208698-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-hSyn-GRAB-gDA3m (#208698). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB-gDA3m plasmid DNA. Syn-driven expression of the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons. These AAV preparations are suitable purity for injection into animals.

Depositor

Yulong Li

Publication

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Promoter

Syn

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only
pcDNA3.3_CoV1_D28

Plasmid

#170447

Purpose

Express SARS-CoV-1 WT spike protein with a 28aa deletion on the C-terminal tail. Used for SARS1 pseudotyped virus production.

Depositor

David Nemazee

Publication

Rogers et al Science. 2020 Aug 21;369(6506):956-963.

Insert

SARS-CoV-1 spike D28 (S SARS-CoV-1)

Tags

NA

Expression

Mammalian

Mutation

last 28aa deletion in c-terminal tail

Promoter

CMV

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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lentiCRISPR - EGFP sgRNA 2

Plasmid

#51761

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

Feng Zhang

Publication

Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

Inserts

Cas9
Puromycin resistance
EGFP sgRNA 2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EFS and U6

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1 SARS-CoV-2 S D614

Plasmid

#158074

Purpose

Expresses SARS-CoV-2 spike protein

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 spike (S )

Expression

Mammalian

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA5/FRT/TO HIS HSPA1A

Plasmid

#19537

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 1A (HSPA1A Human)

Tags

His

Expression

Mammalian

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA5/FRT/TO HIS HSPA8

Plasmid

#19541

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 8 (HSPA8 Human)

Tags

His

Expression

Mammalian

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCS2+ EB3-mKate2

Plasmid

#105940

Purpose

in vitro transcription

Depositor

Caren Norden

Publication

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

EB3 (MAPRE3 Human)

Tags

mKate2

Expression

Bacterial

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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hGAPDH-rLuc

Plasmid

#82479

Purpose

reporter of GAPDH expression

Depositor

Albee Messing

Publication

Cho et al J Neurochem. 2009 Jul;110(1):343-51. Epu

Insert

GAPDH promoter (GAPDH Human)

Use

Luciferase

Expression

Bacterial

Promoter

hGAPDH

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA5/FRT/TO V5 HSPA1A

Plasmid

#19510

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 1A (HSPA1A Human)

Tags

V5

Expression

Mammalian

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA5/FRT/TO V5 HSPA8

Plasmid

#19514

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 8 (HSPA8 Human)

Tags

V5

Expression

Mammalian

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCAGGS SARS-CoV-2 BA.2.12.1 Spike

Plasmid

#186032

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.2.12.1 Spike

Depositor

Marceline Côté

Publication

Gong et al Viruses. 2022 Sep 30;14(10):2178. doi: 1

Insert

SARS-CoV-2 BA.2.12.1 Spike (S )

Expression

Mammalian

Mutation

Contains the BA.2 mutations (T19I, LPPA24S, G142D…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn-GRAB-gDA3m

Plasmid

#208698

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons

Depositor

Yulong Li

Publication

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Has service

AAV1

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3m

Use

AAV

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hSyn-GRAB-gDA3m

Plasmid

#208698

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons

Depositor

Yulong Li

Publication

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Has service

AAV1

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3m

Use

AAV

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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