We narrowed to 34,853 results for: cas

1. pENTR-L3-SpCas9-HF4-2A-EGFP-L2

   Plasmid

   #141049

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   SpCas9-HF4-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-L3-SpCas9-HF2-2A-EGFP-L2

   Plasmid

   #141050

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   SpCas9-HF2-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-Himar-dCas9-mammalian

   Plasmid

   #138414

   Purpose

   Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

   Depositor

   Harris Wang

   Article

   Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

   Insert

   Himar-dCas9
   

   Use

   Tags

   3xFLAG, 6xHis, and SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAWp63-clone3-12 (OptiHF-SpCas9)

   Plasmid

   #131737

   Purpose

   Expresses OptiHF-SpCas9 in mammalian cells

   Depositor

   Alan Siu Lun Wong

   Article

   Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

   Insert

   OptiHF-SpCas9
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Q695A+K848A+E923M+T924V+Q926A

   Promoter

   EFS

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. U6-FnCas12a-TLR-MCV1-gRNA

   Plasmid

   #117412

   Purpose

   FnCas12a-gRNA targeting TLR2.0

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   FnCas12a gRNA targeting TLR 2.0
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT3TS-Hypa-nCas9n-A69

   Plasmid

   #125645

   Purpose

   micro-injection of poly-adenylated Hypa-nCas9n RNA

   Depositor

   Jason Berman

   Article

   Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

   Insert

   Hypa-nCas9n
   

   Use

   Rna in vitro transcription vector

   Tags

   Expression

   Mutation

   N692A, M694A, Q695A, H698A from wild-type Cas9

   Promoter

   T3

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFGRNA-LbCpf1-spCas9-MS2

   Plasmid

   #119675

   Purpose

   Empty vector for cloning LbCpf1-spCas9 MS2-fgRNA

   Depositor

   Yongsub Kim

   Article

   Kweon et al Nat Commun. 2018 Jan 16;9(1):303. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-CMV-TO-Casp-rastvTEV-teTVMV2

   Plasmid

   #116098

   Purpose

   Expresses a combination of proteases and their reporter

   Depositor

   Michael Elowitz

   Article

   Gao et al Science. 2018 Sep 21;361(6408):1252-1258

   Insert

   Casp-rastvTEV-teTVMV2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. C2 G10 png/GFP in casper4

   Plasmid

   #112970

   Purpose

   P element vector for transposon insertion of png-GFP into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Insert

   png-GFP (png Fly)
   

   Use

   P insertion vector

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459V2.0-eSpCas9(1.1)+K855A

    Plasmid

    #108298

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K848A, K855A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459V2.0-eSpCas9(1.1)+K810A

    Plasmid

    #108297

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28b-NmCas9-Flag D16A

    Plasmid

    #109031

    Purpose

    D16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9 (BB)-2A-GFP gamma-tubulin sg

    Plasmid

    #104437

    Purpose

    Knockdown the expression of gamma-tubulin in human cells

    Depositor

    Maria Alvarado-Kristensson

    Article

    Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

    Insert

    gamma-tubulin sgRNA
    

    Use

    Tags

    The gamma-tubulin sgRNA is coexpressed with 3xFLA…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCASCADE-gltA1-gltA2-GapAP1

    Plasmid

    #87150

    Purpose

    gltA1-gltA2-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    gltA1-gltA2-gapAP1 guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-Flag-HFSpCas9-H840A

    Plasmid

    #80455

    Purpose

    high fidelity SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Insert

    HFSpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-SIRPT1-Jatropha curcas

    Plasmid

    #75327

    Purpose

    GST fusion Jatropha curcas Sacsin 1-1304

    Depositor

    Kalle Gehring

    Insert

    Sacsin Jatropha-curcas 1-1304
    

    Use

    Tags

    GST-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pColdII-SIRPT1-Jatropha curcas

    Plasmid

    #75359

    Purpose

    Nt His-tag Jatropha curcas Sacsin 1-1304

    Depositor

    Kalle Gehring

    Insert

    Sacsin Jatropha-curcas 1-1304
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RCAS(B) En cRax deltaC (CC#522)

    Plasmid

    #15195

    Depositor

    Connie Cepko

    Article

    Chen et al Development. 2002 Dec . 129(23):5363-75.

    Insert

    Rax (RAX2 Chicken)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

19. RCAS(B) En cRaxL deltaC (CC#523)

    Plasmid

    #15196

    Depositor

    Connie Cepko

    Article

    Chen et al Development. 2002 Dec . 129(23):5363-75.

    Insert

    RaxL
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

20. RCAS(A) mPax1 T EnR (CT#986)

    Plasmid

    #13893

    Depositor

    Cliff Tabin

    Article

    Brent et al Cell. 2003 Apr 18. 113(2):235-48.

    Insert

    truncated Pax1 (Pax1 Mouse)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2007

    Availability

    Academic Institutions and Nonprofits only