We narrowed to 5,563 results for: cycs

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pCW-Cas9

Plasmid

#50661

Purpose

Doxycycline-inducible lentiviral expression of SpCas9

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Insert

humanized S. pyogenes Cas9

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

Tet ON

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-puro

Plasmid

#39481

Purpose

Tetracycline inducible 3rd generation lentiviral vector

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

V5 and 6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pLBH559_Tet-HisCas14a1Locus

Plasmid

#112502

Purpose

Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

6xHis-TEV

Expression

Bacterial

Promoter

Tet

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLBH545_Tet-Cas14a1_Locus

Plasmid

#112501

Purpose

Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Expression

Bacterial

Promoter

Tet

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLV_hEF1a_rtTA3

Plasmid

#61472

Purpose

3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

Depositor

Ron Weiss

Article

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Insert

rtTA3

Use

Lentiviral

Promoter

hEF1a

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pSOUP

Plasmid

#165419

Purpose

The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

Insert

Rep A

Use

Helper plasmid required for replication for all p…

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLG010 (DRT type 2)

Plasmid

#157888

Purpose

Expresses the DRT type 2 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 2

Expression

Bacterial

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE16

Plasmid

#90357

Purpose

CREB - Cyclic AMP gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

CREB

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBE14

Plasmid

#174653

Purpose

In vivo Cre-lox circularization in E. coli

Depositor

Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Insert

Cre

Use

Synthetic Biology

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pNA0306

Plasmid

#169452

Purpose

pRS415-TEF1p-Cas9-CYC1t; Cas9 expression plasmid

Depositor

Jef Boeke

Article

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

Cas9

Expression

Yeast

Promoter

TEF1p

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLG006 (Ec67 retron)

Plasmid

#157884

Purpose

Expresses the Ec67 retron defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Ec67 retron

Expression

Bacterial

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLG007 (Ec86 retron)

Plasmid

#157885

Purpose

Expresses the Ec86 retron defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Ec86 retron

Expression

Bacterial

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pYZ463

Plasmid

#187971

Purpose

TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pYZ960

Plasmid

#187972

Purpose

TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

TEF1

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pYZ462

Plasmid

#187970

Purpose

TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLG005 (Retron-TIR)

Plasmid

#157883

Purpose

Expresses the retron-TIR defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Retron-TIR

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pYZ959

Plasmid

#192603

Purpose

TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with CEN-URA3

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

ScTEF1

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP

Plasmid

#162823

Purpose

Doxycycline-inducible overexpression of eGFP

Depositor

Matthew Steinhauser

Article

Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Mutation

V163A

Promoter

Tet ON

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLG009 (DRT type 1)

Plasmid

#157887

Purpose

Expresses the DRT type 1 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 1 (RT-nitrilase)

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLG011 (DRT type 3)

Plasmid

#157889

Purpose

Expresses the DRT type 3 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 3 (RT + RT)

Expression

Bacterial

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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