231,398 results

1. pHIV-Luc-ZsGreen

   Plasmid

   #39196

   Depositor

   Bryan Welm

   Inserts

   Firefly Luciferase Luc2P
   ZsGreen
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha and EF1-alpha

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

   Plasmid

   #153004

   Purpose

   Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID-high affinity (N) (G78)
   

   Use

   Lentiviral

   Tags

   FKBP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPRa_all-in-one

   Plasmid

   #183695

   Purpose

   Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDY1173_ADAR1p150

   Plasmid

   #193192

   Purpose

   Express full length ADAR1p150

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

   Insert

   ADAR1p150 (ADAR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV5)

   Viral Prep

   #162379-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   Syn

   Tags

   None

   Available since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

6. FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

   Plasmid

   #58442

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 4 lexA boxes

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

   Plasmid

   #58442

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 4 lexA boxes

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Mm.cargo(Cas9)

   Plasmid

   #174865

   Purpose

   Expresses mouse Peg10 cargoRNA encoding SpCas9

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   SpCas9 flanked by MmPeg10 UTRs
   

   Use

   Tags

   HA-SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPTN-EGFP

   Plasmid

   #27052

   Depositor

   Beatrice Yue

   Article

   Park et al Am J Pathol. 2006 Dec . 169(6):1976-89.

   Insert

   optineurin (OPTN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOPTN-EGFP

    Plasmid

    #27052

    Depositor

    Beatrice Yue

    Article

    Park et al Am J Pathol. 2006 Dec . 169(6):1976-89.

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Orai1 YFP

    Plasmid

    #19756

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    Orai1 (ORAI1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Orai1 YFP

    Plasmid

    #19756

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    Orai1 (ORAI1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMaster12

    Plasmid

    #58527

    Purpose

    Reprogram somatic cells to iPS cells

    Depositor

    Mario Capecchi

    Article

    Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

    Inserts

    neo
    tk
    oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    Myc (MYC Human)
    EBNA-1
    Amp
    Lin28 (LIN28A Human)
    NANOG (NANOG Human)
    IRES
    NR5A2 (NR5A2 Human)
    miR302/367 (MIR367, MIR302A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMaster12

    Plasmid

    #58527

    Purpose

    Reprogram somatic cells to iPS cells

    Depositor

    Mario Capecchi

    Article

    Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

    Inserts

    neo
    tk
    oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    Myc (MYC Human)
    EBNA-1
    Amp
    Lin28 (LIN28A Human)
    NANOG (NANOG Human)
    IRES
    NR5A2 (NR5A2 Human)
    miR302/367 (MIR367, MIR302A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSELIS-RamR

    Plasmid

    #202631

    Purpose

    Enables dual growth and fluorescence based enrichment of RamR biosensors

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    Sh ble
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lambda promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCX-OKS-2A

    Plasmid

    #19771

    Purpose

    Non-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Science. 2008 Nov 7. 322(5903):949-53.

    Insert

    Oct3/4-2A-Klf4-2A-Sox2 (Sox2, Klf4, Pou5f1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Three genes are connected with the foot-and-mouth…

    Promoter

    Available since

    Jan. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCX-OKS-2A

    Plasmid

    #19771

    Purpose

    Non-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Science. 2008 Nov 7. 322(5903):949-53.

    Insert

    Oct3/4-2A-Klf4-2A-Sox2 (Sox2, Klf4, Pou5f1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Three genes are connected with the foot-and-mouth…

    Promoter

    Available since

    Jan. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-sfGFP 150TAG

    Plasmid

    #85493

    Purpose

    pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP 150TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    150 TAG (amino acid 150 mutated to stop codon)

    Promoter

    T7

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-sfGFP 150TAG

    Plasmid

    #85493

    Purpose

    pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP 150TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    150 TAG (amino acid 150 mutated to stop codon)

    Promoter

    T7

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CaMKIIa-hM3D(Gq)-mCherry (AAV1)

    Viral Prep

    #50476-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-hM3D(Gq)-mCherry (#50476). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM3D(Gq)-mCherry plasmid DNA. CaMKIIa-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    CaMKII

    Tags

    None

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only