168,037 results

1. pAAV-CMV-FLEX-SaCas9-U6-sgRosa26

   Plasmid

   #159914

   Purpose

   Mutagenesis of Rosa26 with SauCas9

   Depositor

   Larry Zweifel

   Insert

   Rosa26 gRNA (Gt(ROSA)26Sor Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMCSG53-DsbC

   Plasmid

   #186623

   Purpose

   E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

   Depositor

   Alexei Savchenko

   Article

   Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

   Type

   Empty backbone

   Tags

   DsbC-6His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-hSyn-RiboL1-jGCaMP8s

   Plasmid

   #169247

   Purpose

   AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   RiboL1-jGCaMP8s
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cyto-RA

   Plasmid

   #209860

   Purpose

   Dimerization dependent fluorescent protein RA fused to NES

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddRFP A
   

   Tags

   NES sequence NLVDLQKKLEELELDEQQ

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pR26 CAG/GFP Dest

   Plasmid

   #74281

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Mouse Targeting

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. GZnP2 in pcDNA3.1+

   Plasmid

   #161739

   Purpose

   Cytosolic fluorescent reporter for Zn²⁺ (Kd = 352 pM, ex/em = 488 nm/515 nm)

   Depositor

   Yan Qin

   Article

   Fudge et al ACS Chem Biol. 2018 Jul 20;13(7):1897-19

   Insert

   GZnP2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIVM_26

   Plasmid

   #204501

   Purpose

   Expression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)

   Depositor

   Alessio Ciulli

   Article

   Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

   Insert

   EloB (1-104) and EloC (17-112) (ELOC, ELOB Human)
   

   Expression

   Bacterial

   Mutation

   0

   Promoter

   AmpR

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p28-2iDMD-puro

   Plasmid

   #88895

   Purpose

   28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.

   Depositor

   Michele Calos

   Article

   Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

   Inserts

   Dystrophin (DMD Human)
   luciferase
   puromycin resistance enzyme
   mCherry
   

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMBIS

   Plasmid

   #17817

   Depositor

   Jay Keasling

   Article

   Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

   Insert

   mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. Pepper-fused sgRNA

    Plasmid

    #217520

    Purpose

    Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter

    Depositor

    Xing Li

    Article

    Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

    Insert

    Pepper-fused sgRNA for spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pABE8e-protein

    Plasmid

    #161788

    Purpose

    Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

    Depositor

    David Liu

    Article

    Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

    Insert

    ABE8e base editor
    

    Tags

    8xHIS and BPNLS

    Expression

    Bacterial

    Promoter

    pRhaBAD

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CIp10 - γmGFP (γmGFP based)

    Plasmid

    #163119

    Purpose

    Expresses in Candida albicans

    Depositor

    Patrick Van Dijck

    Article

    Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

    Insert

    gamma mGFP
    

    Use

    Candida albicans expression

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Syn-DIO-NaChBac-dTomato

    Plasmid

    #236252

    Purpose

    Cre dependent NaChBac-TdTomato expression

    Depositor

    Janelia Viral Tools

    Article

    Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

    Insert

    NaChBac-TdTomato
    

    Use

    AAV

    Tags

    TdTomato

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+) ZKSCAN1 MCS Exon Vector

    Plasmid

    #69901

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. TEV Protease-mCherry

    Plasmid

    #243793

    Purpose

    Encodes for mammalian expression of TEV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

    Depositor

    Cole DeForest

    Article

    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

    Insert

    TEV protease
    

    Expression

    Mammalian

    Available Since

    Oct. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. IRF3-V5

    Plasmid

    #169775

    Purpose

    Express IRF3 in mammalian cells

    Depositor

    Sung-Liang Yu

    Article

    Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

    Insert

    Homo sapiens interferon regulatory factor 3 (IRF3), transcript variant 1 (IRF3 Human)
    

    Tags

    V5 tag

    Expression

    Bacterial and Mammalian

    Available Since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid

    #60224

    Purpose

    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA and Rluc-P2A

    Expression

    Mammalian

    Promoter

    EFS, No promoter, and U6

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Ef1a-fDIO hChR2(H134R)-EYFP

    Plasmid

    #55639

    Purpose

    Flp-Dependent ChR2-EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde

    Insert

    hChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn1-eYFP (AAV Retrograde)

    Viral Prep

    #117382-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified pAAV-hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Promoter

    Syn

    Tags

    eYFP

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

20. pHR-scFv-GCN4-mStayGold-GB1-dWPRE

    Plasmid

    #231598

    Purpose

    Express scFv_GCN4-mStayGold for SunTag translation imaging

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    scFv-GCN4-mStayGold
    

    Use

    Lentiviral

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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