303,743 results

1. pcDNA3-ApoE3

   Plasmid

   #197147

   Purpose

   Expresses ApoE3 in Mammalian cell

   Depositor

   Bradley Hyman

   Article

   Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

   Insert

   ApoE3 (APOE Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTriEx-RhoA-wt_mScarlet-i_SGFP2

   Plasmid

   #85071

   Purpose

   Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
   

   Use

   Tags

   6xHis and mScarlet-i and SGFP2

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTriEx-RhoA-wt_mScarlet-i_SGFP2

   Plasmid

   #85071

   Purpose

   Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
   

   Use

   Tags

   6xHis and mScarlet-i and SGFP2

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

   Plasmid

   #221225

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-PcrA M6-UGI-T2A-mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti-dCas9-KRAB-blast

   Plasmid

   #89567

   Purpose

   Plasmid expression dCas9 protein in fusion with KRAB domain

   Depositor

   Gary Hon

   Article

   Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   Blasticidin resistance gene and KRAB

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Lenti-dCas9-KRAB-blast

   Plasmid

   #89567

   Purpose

   Plasmid expression dCas9 protein in fusion with KRAB domain

   Depositor

   Gary Hon

   Article

   Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   Blasticidin resistance gene and KRAB

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-PB1-B/BRISBANE/60/2008

   Plasmid

   #187919

   Purpose

   Expresses PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase basic protein 1
   

   Use

   Tags

   UNTAGGED

   Expression

   Mutation

   Promoter

   CMV Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-PB1-B/BRISBANE/60/2008

   Plasmid

   #187919

   Purpose

   Expresses PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase basic protein 1
   

   Use

   Tags

   UNTAGGED

   Expression

   Mutation

   Promoter

   CMV Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLX-PVX

   Plasmid

   #213040

   Purpose

   PVX vector to express heterologous sequences in plants

   Depositor

   Jose-Antonio Daros

   Article

   Uranga et al Hortic Res. 2023 Dec 19;11(1):uhad279. d

   Insert

   Potato virus X
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S

   Available since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. R5 EMCV

    Plasmid

    #51733

    Purpose

    Dicistronic vector with EMCV IRES

    Depositor

    Vincent Mauro

    Article

    Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

    Insert

    EMCV IRES
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. R5 EMCV

    Plasmid

    #51733

    Purpose

    Dicistronic vector with EMCV IRES

    Depositor

    Vincent Mauro

    Article

    Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

    Insert

    EMCV IRES
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-GfaABC1D-MCS--4x6T-WPRE

    Plasmid

    #196417

    Purpose

    Empty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Type

    Empty backbone

    Use

    AAV; Astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-GfaABC1D-MCS--4x6T-WPRE

    Plasmid

    #196417

    Purpose

    Empty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Type

    Empty backbone

    Use

    AAV; Astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGP-AAV-syn-FLEX-Volton2-ST-WPRE (AAV1)

    Viral Prep

    #172907-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-Volton2-ST-WPRE (#172907). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-Volton2-ST-WPRE plasmid DNA. Cre-dependent, Syn-driven expression of the voltage sensor Voltron2-ST containing a soma localization targeting sequence. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Promoter

    Syn

    Tags

    None

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

15. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid

    #201917

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and Human U6

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid

    #201917

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and Human U6

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX6P1-GFP-Nanobody

    Plasmid

    #61838

    Purpose

    Expresses GST-tagged anti-GFP Nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    GFP-Nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX6P1-GFP-Nanobody

    Plasmid

    #61838

    Purpose

    Expresses GST-tagged anti-GFP Nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    GFP-Nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 014LP-pAAV-ANF-657- Cre-pA

    Plasmid

    #120279

    Purpose

    AAV vector for Atrial specific Cre recombinase expression

    Depositor

    Xander Wehrens

    Article

    Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

    Insert

    Cre
    

    Use

    AAV

    Tags

    CRE

    Expression

    Mutation

    Promoter

    ANF promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 014LP-pAAV-ANF-657- Cre-pA

    Plasmid

    #120279

    Purpose

    AAV vector for Atrial specific Cre recombinase expression

    Depositor

    Xander Wehrens

    Article

    Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

    Insert

    Cre
    

    Use

    AAV

    Tags

    CRE

    Expression

    Mutation

    Promoter

    ANF promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge