We narrowed to 16,461 results for: LEA
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Plasmid#41728DepositorInsertNotch-1 Full Length (Notch1 Mouse)
Tags6xMycExpressionMammalianMutationL1997V; Contains murine Notch-1 protein up to ami…Available SinceDec. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
iCas
Plasmid#84232PurposeExpression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)DepositorInsertCas9
Tags2A-OFP co-expression and ERT2-ERT2ExpressionMammalianPromoterCMVAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-MBP-TEV
Plasmid#69929Purposebacterial expression of MBP-TEV n-terminal fusionsDepositorHas ServiceCloning Grade DNATypeEmpty backboneTagsMBP and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceJan. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG mito-GCaMP5G
Plasmid#105009Purposemitochondrial calcium sensorDepositorInsertGCaMP5G
ExpressionMammalianPromoterCAGAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-FB-orig BA
Plasmid#13420DepositorInsertorig BA
TagsGal11PExpressionBacterialAvailable SinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLVX-XBP1 mNeonGreen NLS
Plasmid#115968PurposeER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreenDepositorInsertX-box binding protein 1 (XBP1 Synthetic, Human)
UseLentiviralTagsHA tag, c-myc NLS, and mNeonGreenExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK7WGF2::hCas9
Plasmid#46965PurposeExpresses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissueDepositorInserthCas9
UseCRISPR; Plant expressionTagseGFPPromoter35SAvailable SinceAug. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK792
Plasmid#8830DepositorInsertTEV protease, S219P mutant
TagsHis, MBP (cleaved), and polyarginineExpressionBacterialMutationS219P mutationAvailable SinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGP-FF
Plasmid#13480DepositorTypeEmpty backboneTagsGal11PExpressionBacterialAvailable SinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEJS578_DD-dSpyCas9-mCherry-APEX2
Plasmid#108570PurposeExpression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factorsDepositorInsertdSpyCas9
UseCRISPR and LentiviralTagsAPEX2, Destabilization domains, Flag, and mCherry…ExpressionMammalianPromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF6.mCherry-TSG101
Plasmid#38318DepositorAvailable SinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTE4398
Plasmid#74042PurposeExpresses human codon-optimized LbCpf1 and Lb crRNA.DepositorInsertsLb crRNA
LbCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianPromoterCMV and human U6Available SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTP1122
Plasmid#104159PurposeBacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)DepositorInsertAnti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
ER-GCaMP6-150
Plasmid#86918PurposeFluorescent reporter for ER calcium signalingDepositorInsertER-GCaMP6-150
UseLentiviralExpressionMammalianMutationK78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…PromoterCAMKIIAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 hPGK-PuroR-pA donor
Plasmid#22072DepositorInsertPGK-Puro (PGK1 Human)
UseZfn donorAvailable SinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-FLAG/HA Ago4
Plasmid#10824DepositorAvailable SinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pk7-GR-GFP
Plasmid#15534DepositorInsertGlucocorticoid receptor (NR3C1 Human)
TagsGFPExpressionMammalianMutationThe glucocorticoid receptor was cloned into the X…Available SinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flpo
Plasmid#55637PurposeExpresses Flpo in Mammalian CellsDepositorHas ServiceAAV Retrograde, AAV1, and AAV2InsertFlpo
UseAAVExpressionMammalianPromoterEf1aAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (Neo)
Plasmid#89180PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…ExpressionMammalianPromoterTREAvailable SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-XBP1s
Plasmid#63680PurposeMammalian expression of Flag tagged XBP1sDepositorAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only