We narrowed to 3,940 results for: SPL

1. pNAS1b split mCherry NEDD4 WW2 neg ctrl

   Plasmid

   #112030

   Purpose

   Mode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide)

   Depositor

   Jesse Rinehart

   Article

   Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

   Insert

   NEDD4 WW2 domain fused to C-terminal split mCherry, neg ctrl interacting peptide fused to N-terminal split mCherry
   

   Use

   Tags

   Split mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. T2A Split/Link TurboID Kit

   Plasmid Kit

   #1000000249

   Purpose

   The T2A Split/Link TurboID Kit uses a sequential Golden Gate assembly method and can be used to assemble mammalian expression vectors for proximity labeling experiments.

   Depositor

   Sven Eyckerman

   Available since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

3. MoClo Yeast Secretion and Display (YSD) Toolkit

   Plasmid Kit

   #1000000240

   Purpose

   The YSD Toolkit is a collection of 34 plasmids that can be used in conjunction with the MoClo Yeast Toolkit (YTK) to optimize secretion and cell surface display of proteins of interest in yeast.

   Depositor

   Paul Young

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

4. BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron

   Plasmid

   #235087

   Purpose

   GFP splicing reporter with FRG1 intron

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron (FRG1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   custom bidirectional

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron

   Plasmid

   #235086

   Purpose

   GFP splicing reporter with CORO1B intron

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron (CORO1B Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   custom bidirectional

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)

   Plasmid

   #190112

   Purpose

   AAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Inserts

   MMLV-RT(dRH)
   U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)
   

   Use

   AAV

   Tags

   bpNLS and bpNLS-P2A-eGFP

   Expression

   Mutation

   mutations from RT in PE2 and truncation of RNAse …

   Promoter

   EFS and U6, H1

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDropIn-T2A-split-intein-Gal4[21-881]-C-int-G418R

   Plasmid

   #199198

   Purpose

   Insert to create split-intein Gal4[21-881]-C-int knock-in vectors, with G418 resistance, via Drop-In cloning, released via BsmBI digest

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[21-881]-C-int-G418R
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-BDLacZ-SAS620-3'Luciferase (split CAGGT)-SV40pA

   Plasmid

   #216321

   Purpose

   Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

   Depositor

   Elvir Becirovic

   Article

   Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

   Insert

   Split Luciferase + splice acceptor site
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pzk490-pmax-NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]

   Plasmid

   #196843

   Purpose

   Express NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Insert

   NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBBK04 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitHygR,AmpR)

    Plasmid

    #178988

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR)

    Plasmid

    #178986

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBBK10 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitURA3,AmpR)

    Plasmid

    #178994

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR)

    Plasmid

    #178992

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)

    Plasmid

    #178990

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Splicing-targeting CRISPR-Cas9 library for human lncRNAs

    Pooled Library

    #119977

    Purpose

    The CRISPR sgRNA library was designed to target the 5' or 3' splice sites of lncRNAs in genome-scale, in order to knockout the targeted lncRNAs.

    Depositor

    Wensheng Wei

    Article

    Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

16. pHBS1178 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 dCTD EcoRV)

    Plasmid

    #107860

    Purpose

    To quickly insert CTD mutants into TDP43 splicing reporter

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of CTD in TDP43 splicing reporter, inser…

    Promoter

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pHBS1333 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 G368W W385G)

    Plasmid

    #107855

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G368W and W385G in splicing reporter

    Promoter

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. KHBD00510

    Plasmid

    #39611

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8365 (E(spl)m8-HLH Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

19. KHBD00413

    Plasmid

    #34520

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8365 (E(spl)m8-HLH Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

20. KHBD00021

    Plasmid

    #34183

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG14548 (E(spl)mbeta-HLH Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only