We narrowed to 566 results for: prime editing

Showing: 321 - 340 of 566 results

Results per page:

Sort results by:

pT7-MLH1dn (mouse) for IVT

Plasmid

#246485

Purpose

Template for in vitro transcription of mouse MLH1dn

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

mouse MLH1dn (Mlh1 Mouse)

Use

Template for in vitro transcription

Mutation

deletion of residues 758-760

Promoter

T7

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVNP2.0-SpCas9

Plasmid

#206880

Purpose

Expresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domain

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

SpCas9 (cas9 )

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-CMV-MLH1dn-IRES-puroR

Plasmid

#207354

Purpose

Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

MutL homolog 1 (MLH1 Human)

Use

Lentiviral

Mutation

Δ754-756 dominant negative mutant

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
fluoPEER-GFP>BFP

Plasmid

#185477

Purpose

Fluorescent reporter for a Y66H mutation to convert a GFP to BFP.

Depositor

Sabine Fuchs

Article

Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

Insert

GFP-Y66-editing-site

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
fluoPEER-HEK3_5bp-del

Plasmid

#185476

Purpose

Fluorescent reporter for a 5 basepair deletion at the HEK3 locus.

Depositor

Sabine Fuchs

Article

Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

Insert

HEK3 editing site

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PE6d

Plasmid

#207854

Purpose

Mammalian expression of PE6d prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6d

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PE6e

Plasmid

#207855

Purpose

Mammalian expression of PE6e prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6e

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, K918A, K775R)

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PEmaxRNaseHtrunc

Plasmid

#207858

Purpose

Mammalian expression of PEmaxDRNaseH prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PEmaxDRNaseH

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

M-MLVRTDRNAseHrt

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SpyCas9 PE2*-3HA

Plasmid

#169854

Purpose

Mammalian Expression, SpyCas9 PE2* prime editor with 3HA-tag

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SpCas9-H840A-star-M-MLV-3HA

Tags

3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…

Expression

Mammalian

Promoter

cmv

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-EF1a-hMLH1dn-eGFP

Plasmid

#191104

Purpose

Lentiviral expression plasmid of hMLH1dn with P2A-eGFP reporter

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
QPM-sgR (pTaU3)

Plasmid

#140448

Purpose

For sgRNA expression in monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

Insert

TaU3-sgRNA

Use

CRISPR

Expression

Plant

Promoter

TaU3

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-EGFR-L858R-tmpknot-epegRNA

Plasmid

#214092

Purpose

Lentiviral vector expressing epegRNA to induce EGFR L858R mutation

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

EGFR L858R epegRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-Sp-gRNA-AAVS1_B3903c_attP

Plasmid

#182144

Purpose

To insert attP attachment site at AAVS1 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

AAVS1_B3903c_attP pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-Sp-gRNA-AAVS1_A3786c_attP

Plasmid

#182143

Purpose

To insert attP attachment site at AAVS1 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

A3786c-attP pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiV2-hU6-evopreQ1

Plasmid

#210189

Purpose

pegRNA/ngRNA library cloning

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Type

Empty backbone

Use

Lentiviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-Sp-gRNA-CCR5_DF_B584b_attB

Plasmid

#182148

Purpose

To insert attB attachment site at CCR5 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

CCR5-B584b-attB pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-Sp-gRNA-CCR5_DF_A509b_attB

Plasmid

#182147

Purpose

To insert attB attachment site at CCR5 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

CCR5-A509b-attB pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiV2-mU6-evopreQ1

Plasmid

#210190

Purpose

pegRNA/ngRNA library cloning

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Type

Empty backbone

Use

Lentiviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
attB-puro-GA donor

Plasmid

#182138

Purpose

attB-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attB-GA-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-KRAS-G12C-tmpknot-epegRNA

Plasmid

#214094

Purpose

Lentiviral vector expressing epegRNA to induce KRAS G12C mutation

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

KRAS G12C epegRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge