229,932 results

1. pGP-CMV-GCaMP6s

   Plasmid

   #40753

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Insert

   GCaMP6s
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-Cdk9

   Plasmid

   #28102

   Depositor

   Andrew Rice

   Article

   Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

   Insert

   Cdk9 (CDK9 Human)
   

   Use

   Tags

   3x HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-Cdk9

   Plasmid

   #28102

   Depositor

   Andrew Rice

   Article

   Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

   Insert

   Cdk9 (CDK9 Human)
   

   Use

   Tags

   3x HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPK1520

   Plasmid

   #65777

   Purpose

   Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SpCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK1520

   Plasmid

   #65777

   Purpose

   Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SpCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-AAV-syn-jGCaMP8f-WPRE (AAV1)

   Viral Prep

   #162376-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP8f-WPRE (#162376). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8f-WPRE plasmid DNA. Syn-driven expression of ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   Syn

   Tags

   None

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

7. pLIB-FLAG(N)-RPB1

   Plasmid

   #231722

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Insert

   POLR2A (POLR2A Human)
   

   Use

   Tags

   Flag-3C

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGreenFire1_E2F1RE

   Plasmid

   #112248

   Purpose

   expression of GFP and Luciferase under the control of E2F1 responsive element

   Depositor

   Simone Di Giovanni

   Insert

   E2F1RE (responsive element)
   

   Use

   Lentiviral

   Tags

   copGFP and luciferase

   Expression

   Mutation

   Promoter

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVS1

   Plasmid

   #33143

   Depositor

   Michael Rosbash

   Article

   Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

   Insert

   LacO (256 copies)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pVS1

    Plasmid

    #33143

    Depositor

    Michael Rosbash

    Article

    Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

    Insert

    LacO (256 copies)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

11. 8xNFAT-ZsG-hCD4

    Plasmid

    #162746

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with human CD4 gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Front Endocrinol (Lausanne). 2021 Mar 25

    Insert

    8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Cre:GFP

    Plasmid

    #13776

    Purpose

    Mammalian expression of Cre recombinase fused to GFP

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre-GFP fusion protein
    

    Use

    Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Cre:GFP

    Plasmid

    #13776

    Purpose

    Mammalian expression of Cre recombinase fused to GFP

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre-GFP fusion protein
    

    Use

    Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-CreERT2

    Plasmid

    #139547

    Purpose

    CreERT2 ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    CreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. gRNA-MS2x2-TagRFP657

    Plasmid

    #184441

    Purpose

    sgRNA with MS2 aptamer, TagRFP657 as selection marker

    Depositor

    Rhys Allan

    Article

    Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

    Insert

    sgRNA-MS2x2, TagRFP657
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-HA-Nrxn1beta AS4(-)

    Plasmid

    #59409

    Purpose

    Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

    Depositor

    Peter Scheiffele

    Article

    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

    Insert

    Nrxn1beta AS4(-) (Nrxn1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-HA-Nrxn1beta AS4(-)

    Plasmid

    #59409

    Purpose

    Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

    Depositor

    Peter Scheiffele

    Article

    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

    Insert

    Nrxn1beta AS4(-) (Nrxn1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pES001 MBP-GSK3β-HA-His

    Plasmid

    #154010

    Purpose

    Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    GSK3B (GSK3B Human)
    

    Use

    Tags

    HA tag, His6, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMK462 (Hygro-P2A-mAB)

    Plasmid

    #214388

    Purpose

    mAID-BromoTag for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Hygro-P2A-mAID-BromoTag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pProEx-HTa-SOD1 WT

    Plasmid

    #191849

    Purpose

    express wild-type SOD1 in bacterial cells

    Depositor

    Nam-Chul Ha

    Article

    Baek et al Commun Biol. 2022 Oct 12;5(1):1085. doi:

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge