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We narrowed to 6,770 results for: GFP expression plasmids

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pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST

Plasmid

#127332

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1079 - pAAV TH gRNA A EF1a EGFP

Plasmid

#113159

Purpose

An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat TH

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159672

Purpose

Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

βTub-NLS-hSpCas9-T2A-GFP

Use

Tags

T2A-GFP

Expression

Insect

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159671

Purpose

Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

ExuL-NLS-hSpCas9-T2A-GFP

Use

Tags

T2A-GFP

Expression

Insect

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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BCL6-GFP (1-275AA) in Cilantro

Plasmid

#169932

Purpose

Overexpression of 1-275AA BCL6-GFP fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

Insert

BCL6 1-275AA (BCL6 Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

160R>C

Promoter

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR1-deGFP Y35TAG D193TAG-6xHis-T500

Plasmid

#92227

Purpose

Expression of deGFP mutant Y35TAG D193TAG for ncAA incoporation using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

deGFP Y35TAG D193TAG

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Mutation

Residues Y35 D193 were mutated into TAG (Amber) s…

Promoter

p70a

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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px552-U6-gRNA1-U6-gRNA2-CMV-eGFP

Plasmid

#211760

Purpose

Paired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.

Depositor

Glenn Yiu

Article

Chung et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

Insert

gRNA1 and gRNA2 targeting VEGF-A (VEGFA Human, Mouse, M. mulatta (rhesus macaque))

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GSDMD-eGFP-SV40p-BlasR

Plasmid

#228254

Purpose

pLenti-CMV-GSDMD-eGFP-SV40p-BlasR plasmid for ectopic expression of human full-length GSDMD fusion with GFP

Depositor

Judy Lieberman

Article

Fontana et al Cell. 2024 Sep 2:S0092-8674(24)00898-5.

Insert

GSDMD (GSDMD Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Jaws-KGC-GFP-ER2

Plasmid

#65015

Purpose

AAV-mediated expression of Jaws under the CaMKII promoter

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

CaMKII

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-Jaws-KGC-GFP-ER2

Plasmid

#65014

Purpose

AAV-mediated expression of Jaws under the human synapsin promoter

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Has Service

AAV Retrograde, AAV5, and AAV8

Insert

Jaws-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

human synapsin

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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anti-GFP scFv [N86/38] with sortase tag

Plasmid

#204419

Purpose

Mammalian Expression of anti-GFP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N86/38.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

Insert

Recombinant mouse scFv targeting GFP (Aequorea victoria)

Use

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-ubiquitin-EphB2-SV40-GFP (B2G)

Plasmid

#65442

Purpose

Expression of mouse EPHB2 and GFP in mammalian cells

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

EPHB2 (Ephb2 Mouse)

Use

Lentiviral

Tags

SV40-EGFP

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

UBQ

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pOTTC710 - pAAV EF1a DIO Mem-AcGFP

Plasmid

#75081

Purpose

An AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

Insert

Mem-AcGFP

Use

AAV and Cre/Lox

Tags

Palmitylation site

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2

Plasmid

#196250

Purpose

Plasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

attL1-GlpT-scGFP-attL2

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119 promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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LZF1826 pAAV_hSyn-DiO-SomArchon-EGFP-P2A-stGtACR2

Plasmid

#135412

Purpose

simultaneous optical recording and manipulation of electrical activity in neurons

Depositor

Adam Cohen

Article

Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

Insert

SomArchon-EGFP-P2A-stGtACR2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Flex-PAK1-AID-ires-EGFP

Plasmid

#83489

Purpose

cre-dependent PAK1-AID under EF1a promoter

Depositor

Dong Kong

Article

Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

Insert

PAK1-AID-ires-EGFP

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-pri-miR-137

Plasmid

#235159

Purpose

Expresses EGFP and miR-137 primary transcript in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/pri-miR-137 (Mir137 Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs

Plasmid

#195018

Purpose

An adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1

Depositor

Christopher Richie

Article

Belilos et al bioRxiv [Preprint]. 2023 Oct 9:2023.02.0

Inserts

CCGTATAGGCGGCTGCCCAA
EGFP-KASH
TTCCGTGGTGGGCAACGTAG

Use

AAV

Tags

KASH domain of Nesprin2

Expression

Mutation

Promoter

EF1a, hU6, and mU6

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-GFP-Fishell-1

Plasmid

#83900

Purpose

GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

mDlx

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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VI_pAAV-ProA7-GFP-WPRE

Plasmid

#125891

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA7

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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