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pscALPS-HIV-1 Group P-vpr

Plasmid

#115837

Purpose

Encodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV1 Group P Vpr (HQ179987)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-HIV-1 Group P-vpr

Plasmid

#115837

Purpose

Encodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV1 Group P Vpr (HQ179987)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0336 pAAV EFS-HvsCas7-11 for dual vector

Plasmid

#172515

Purpose

Encodes HvsCas7-11 mammalian expression casette in AAV backbone

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pAAV EFS-HvsCas7-11 for dual vector

Use

AAV

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0336 pAAV EFS-HvsCas7-11 for dual vector

Plasmid

#172515

Purpose

Encodes HvsCas7-11 mammalian expression casette in AAV backbone

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pAAV EFS-HvsCas7-11 for dual vector

Use

AAV

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172505

Purpose

Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-E

Plasmid

#215814

Purpose

Expresses SARS-CoV-2 Envelope protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Envelope (E )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-SARS-CoV-2-M

Plasmid

#215813

Purpose

Expresses SARS-CoV-2 Membrane protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Membrane (M )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-SARS-CoV-2-S (D614G)

Plasmid

#215838

Purpose

Expresses SARS-CoV-2 Spike protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Spike (S )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172505

Purpose

Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-SARS-CoV-2-E

Plasmid

#215814

Purpose

Expresses SARS-CoV-2 Envelope protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Envelope (E )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-SARS-CoV-2-M

Plasmid

#215813

Purpose

Expresses SARS-CoV-2 Membrane protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Membrane (M )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-SARS-CoV-2-S (D614G)

Plasmid

#215838

Purpose

Expresses SARS-CoV-2 Spike protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Spike (S )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
G1397 DddAtox-N–dSpCas9

Plasmid

#157835

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Use

CRISPR

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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G1397 DddAtox-N–dSpCas9

Plasmid

#157835

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Use

CRISPR

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS gag-gfp/vpx

Plasmid

#115808

Purpose

SFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpx

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gag-gfp

Use

Lentiviral

Promoter

SFFV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS gag-gfp/vpx

Plasmid

#115808

Purpose

SFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpx

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gag-gfp

Use

Lentiviral

Promoter

SFFV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2-HA-MKK3

Plasmid

#21580

Depositor

John Kyriakis

Article

Molnar et al J Biol Chem. 1997 May 16. 272(20):13229-

Insert

mitogen-activated protein kinase kinase 3 (MAP2K3 Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pMT2-HA-MKK3

Plasmid

#21580

Depositor

John Kyriakis

Article

Molnar et al J Biol Chem. 1997 May 16. 272(20):13229-

Insert

mitogen-activated protein kinase kinase 3 (MAP2K3 Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pscALPS-HIV-1 Group O-vpr

Plasmid

#115836

Purpose

Encodes codon optimized 3xFLAG-HIV1 Group O Vpr (L20571) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV1 Group O Vpr (L20571)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-HIV-1 Group O-vpr

Plasmid

#115836

Purpose

Encodes codon optimized 3xFLAG-HIV1 Group O Vpr (L20571) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV1 Group O Vpr (L20571)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge