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pMY.001

Plasmid

#228680

Purpose

pTDH3 Promoter for yeast expression with Nterminal tag

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

pTDH3

Expression

Yeast

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-7)-neg_tag1

Plasmid

#219419

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

GFP(-7)-neg_tag1

Tags

DEEEDD and MGHHHHHGG

Expression

Bacterial

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-mBlueberry(-9)

Plasmid

#219416

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

mBlueberry2(-9)

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

K137E, K199E, R234E

Promoter

T7

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-PspCas13b_gRNA[ccdbCam]-2xPP7

Plasmid

#196846

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 2xPP7

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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gzk328-dpspCas13b_gRNA[ccdbCam]-10xPBSc_Loop

Plasmid

#196844

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 10xPBSc_Loop

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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gzk326-dpspCas13b_gRNA[ccdbCam]-5xPBSc_Loop

Plasmid

#196845

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 5xPBSc_Loop

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMYT006

Plasmid

#203322

Purpose

Encodes pLacHTA1 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

pLacHTA1

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMYT082

Plasmid

#198864

Purpose

Markerless yeast integration Vector at Int.8 locus to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMYT076

Plasmid

#180729

Purpose

Markerless yeast integration Vector at Int.2 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT083

Plasmid

#180736

Purpose

Markerless yeast integration Vector at Int.9 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT084

Plasmid

#180737

Purpose

Markerless yeast integration Vector at Int.10 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT081

Plasmid

#180734

Purpose

Markerless yeast integration Vector at Int.7 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT080

Plasmid

#180733

Purpose

Markerless yeast integration Vector at Int.6 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMYT079

Plasmid

#180732

Purpose

Markerless yeast integration Vector at Int.5 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT078

Plasmid

#180731

Purpose

Markerless yeast integration Vector at Int.4 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT077

Plasmid

#180730

Purpose

Markerless yeast integration Vector at Int.3 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT075

Plasmid

#180728

Purpose

Markerless yeast integration Vector at Int.1 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT056

Plasmid

#180709

Purpose

Assembly cassette with ConL9 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT055

Plasmid

#180708

Purpose

Assembly cassette with ConL8 and ConR9 connectors to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMYT054

Plasmid

#180707

Purpose

Assembly cassette with ConL8 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge