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We narrowed to 1,533 results for: lentiviral backbone

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Showing: 341 - 360 of 1533 results

  1. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118154-plasmid-map-sequence-id-239465

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  2. pHAGE N-mRuby3 (from SARS-CoV-2) IRES puro

    Plasmid
    #170466
    Purpose
    The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mRuby3 fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance
    Depositor
    Raphael Gaudin
    Article
    Bakhache et al bioRxiv 2021
    Insert
    Nucleocapsid
    Use
    Lentiviral
    Tags
    mRuby3
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    170466-plasmid-map-sequence-id-345815

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  4. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid
    #118156
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available Since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    118156-plasmid-map-sequence-id-239644

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  5. lentiCRISPR - Amplicon, BCR/ABL sgRNA 1

    Plasmid
    #70659
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    70659-plasmid-map-sequence-id-162503

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  8. pHL047 [15ARE23bp -p(TATA)-fLuc-UBC-rLuc-P2A-GFP]

    Plasmid
    #246422
    Purpose
    Firefly luciferase driven by an AHR responsive minimal promoter and a renilla luciferase and GFP driven by a constitutive UBC promoter flipped with respect to the lentiviral backbone
    Depositor
    Marco Jost
    Article
    Verma et al bioRxiv [Preprint]. 2025 Sep 15:2025.09.
    Inserts
    Firefly luciferase
    Renilla luciferase-P2A-EGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    AHR responsive minimal promoter and UbC
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    246422-plasmid-map-sequence-id-488911

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  10. lentiCRISPR - EGFP sgRNA 4

    Plasmid
    #51763
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 4
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51763-plasmid-map-sequence-id-79382

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  12. lentiCRISPR - C9orf114 sgRNA 1

    Plasmid
    #70655
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C9orf114
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. lentiCRISPR - C9orf114 sgRNA 2

    Plasmid
    #70654
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C9orf114
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. lentiCRISPR - C3orf17 sgRNA 1

    Plasmid
    #70652
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C3orf17
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pCVL.SSA TLR (Ani)

    Plasmid
    #45577
    Purpose
    Codes for the SSA-TLR with the target site for I-Ani I in a lentiviral backbone
    Depositor
    Andrew Scharenberg
    Article
    Kuhar et al Nucleic Acids Res. 2013 Oct 10.
    Inserts
    5' iRFP arm
    eGFP with I-Ani I TS
    +3 mCherry
    3' iRFP arm
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    embedded I-Ani I TS from 163-185bp, truncated 25 …
    Promoter
    SFFV
    Available Since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    45577-plasmid-map-sequence-id-67848

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  16. FRIG-myc-Sema4D-CD4

    Plasmid
    #51606
    Purpose
    expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone
    Depositor
    Suzanne Paradis
    Article
    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do
    Insert
    Sema4D/CD4-FLAG (Sema4d Mouse, Human)
    Use
    Lentiviral
    Tags
    FLAG and myc
    Expression
    Mammalian
    Mutation
    Sema4D amino acids 660-861 were replaced with CD4…
    Promoter
    RSV
    Available Since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pFUGW-hGtACR2-EYFP

    Plasmid
    #67877
    Purpose
    Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone
    Depositor
    John Spudich
    Article
    Govorunova et al Science. 2015 Jun 25. pii: aaa7484.
    Insert
    hGtACR2
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Promoter
    UbC
    Available Since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    67877-plasmid-map-sequence-id-199004

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Showing: 341 - 360 of 1533 results