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Plasmid#153006PurposeExpresses split-TurboID N-terminal fragment targeted to outer mitochondrial membraneDepositorInsertOMM (Tom20)-sTurboID (N) (L73)
UseLentiviralTagsFKBP and V5ExpressionMammalianPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCasAb-apr
Plasmid#121998PurposeBacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumanniiDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0040-LwaCas13a crRNA backbone
Plasmid#103851PurposeBackbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertU6-LwaCas13aDR-BbsI-BbsI-polyT
UseCRISPRExpressionMammalianPromoterU6Available sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-dCas9-VP64-6xHis
Plasmid#62935PurposeExpression of dCas9-VP64-6xHis in bacterial cellsDepositorInsertdCas9-VP64
Tags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A and H840A amino acid changes render Cas9 nuc…PromoterT7Available sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLJM1-FIRE-pHLy
Plasmid#170775PurposeRatiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterCMVAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Nb-gp41-GFP (MoonTag-Nb-GFP)
Plasmid#128602PurposeExpresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).DepositorInsertNanobody_gp41
UseLentiviralTagsGB1 and sfGFPPromoterSFFVAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ERKKTRClover
Plasmid#59138PurposeORF encoding ERK KTR mClover flanked by Gateway sequencesDepositorInsertERK Kinase Translocation Reporter (MAPK1 Human, Mouse)
Available sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
T444T
Plasmid#113081PurposeEnhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.DepositorTypeEmpty backboneUseRNAiExpressionBacterialAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mini-pHelper-1.0
Plasmid#230933PurposeThe mini version (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV productionDepositorTypeEmpty backboneUseAAVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZR071_SFFV-dCas9-mCherry-KRAB
Plasmid#180264PurposeLentiviral expression vector for dCas9-KRAB in mammalian cellsDepositorInsertdCas9
UseCRISPR and LentiviralTagsKRAB and mCherryExpressionMammalianMutationNuclease dead Cas9PromoterSFFVAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-pCMV-PfV-GS-Sapphire
Plasmid#116933PurposeMammalian vector for expression of the 40nm GEMDepositorInsertPfv
TagsSapphireExpressionMammalianPromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-ZO1-C-14
Plasmid#55165PurposeLocalization: Tight Junctions, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertZO1 (TJP1 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSIN-PAmCherry-KFERQ-NE
Plasmid#102365PurposeLentiviral overexpression of PA-mCherry-KFERQ fusionDepositorInsertsPA-mCherry
KFERQ peptide
UseLentiviralTagsNEExpressionMammalianPromoterEF-1aAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-CibN-dCas9-CibN
Plasmid#60553PurposeExpresses CibN-dCas9-CibN in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-CibN
UseCRISPRTagsFLAG and SV40 NLSExpressionMammalianMutationinactivating Cas9 mutations D10A and H840APromoterCMVAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRKH3-slpGFP
Plasmid#27168DepositorInsertsurface (S)-layer protein promoter region fused with modified green fluorescent protein 5
ExpressionBacterialAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pY109 (lenti-LbCpf1)
Plasmid#84740PurposeLenti virus delivery of LbCpf1 and crRNA guideDepositorInsertshuLbCpf1
puromycin resistance gene
UseLentiviralTags3xHA, NLS, and P2APromoterEFSAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-syn-jGCaMP8f-WPRE
Plasmid#162376PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoterDepositorHas serviceAAV1, AAV5, AAV9, and AAV RetrogradeInsertjGCaMP8f
UseAAVTags6xHisExpressionMammalianMutationQ315LPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLTS
Plasmid#59386PurposeExpresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.DepositorPublicationTypeEmpty backboneUseGenome engineeringExpressionBacterialAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
v3em-Cterm-PE2max-∆RNaseH-dualU6
Plasmid#198735PurposeAAV genome encoding C-terminal PE2max and U6 expression cassettesDepositorInsertNpuC-CtermPE2max∆RNaseH
UseAAVPromoterCbhAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SpyTag003-sfGFP
Plasmid#133454PurposeExpresses SpyTag003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyTag003-sfGFP
TagsGSGSGS linker and His6ExpressionBacterialPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by