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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,352 results for: Cas9

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Showing: 3601 - 3620 of 14352 results

  3. pSpCas9(BB)-2A-Blast_HP1beta_gRNA

    Plasmid
    #127908
    Purpose
    WT Cas9 Vector with Blasticidin Selection Marker targeting the 5' end of the human HP1beta gene
    Depositor
    Mark Groudine
    Insert
    gRNA for Human 5' HP1beta
    Use
    CRISPR
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127908-plasmid-map-sequence-id-249780

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  6. pvPE-V3

    Plasmid
    #240287
    Purpose
    Encodes third generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.
    Depositor
    Lusheng Huang
    Publication
    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pvPE-V3
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240287-plasmid-map-sequence-id-488808

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  11. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid
    #174139
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174139-plasmid-map-sequence-id-341913

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  12. Sc++

    Plasmid
    #155011
    Purpose
    Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells
    Depositor
    Joseph Jacobson
    Publication
    Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sc++
    Expression
    Mammalian
    Mutation
    T1227K, Loop Substitution from S. anginosus
    Promoter
    EF1ɑ
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155011-plasmid-map-sequence-id-308829

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  17. gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

    Plasmid
    #217662
    Purpose
    expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL
    Depositor
    Daniel Gerlich
    Publication
    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)
    Use
    CRISPR
    Expression
    Bacterial and Mammalian
    Promoter
    U6
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217662-plasmid-map-sequence-id-440854

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  20. pPD292 PPH/HBB_IVS2/-T2A-dCas9-NFZ-P2A-BFP

    Plasmid
    #249156
    Purpose
    Overexpression of PPH-T2A-dCas9-NFZ-P2A-BFP with HBB IVS2 intron in PPH coding sequence and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Publication
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    PPH/HBB_IVS2/-T2A-dCas9-NFZ-P2A-mTagBFP2 (HBB Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249156-plasmid-map-sequence-id-496442

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Showing: 3601 - 3620 of 14352 results