We narrowed to 34,882 results for: CaS;

1. pdCas9-DNMT3A-PuroR (ANV)_v2

   Plasmid

   #74408

   Purpose

   Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; E756A inactiva…

   Promoter

   CBh

   Available since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PET-eSpCas9-plus-NLS-6xHis

   Plasmid

   #207380

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, R1060A, amino acids 1005-1013 replaced wit…

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHACK(Gal4)-DONR(T2A-Cas9)

   Plasmid

   #194768

   Purpose

   To insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Insert

   T2A-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pdCas9+sgRNA expression vector precursor

   Plasmid

   #171798

   Purpose

   PICASSO: Library expression vector precursor for paired dCas9+sgRNA expression

   Depositor

   Stephen Elledge

   Article

   Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO

   Available since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. XLone-BSD Cas13d P2A mCherry

   Plasmid

   #155183

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of Cas13d and mCherry

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Insert

   CasRx
   

   Use

   Unspecified; Piggybac transposon

   Tags

   NLS and P2A mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p2T-cmv-SpCas9-TadDE-BlastR

   Plasmid

   #193850

   Purpose

   Express TadDE in mammalian cells with BlastR selection, Tol2 integration

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadDE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-E1347A-nCas9(D10A)-UGI

   Plasmid

   #187410

   Purpose

   DddA E1347A variant

   Depositor

   Jin-Soo Kim

   Article

   Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

   Insert

   DddA E1347A variant
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CMV-FLEX-SaCas9-U6-sgNts

   Plasmid

   #159909

   Purpose

   Mutagenesis of Nts

   Depositor

   Larry Zweifel

   Insert

   Nts (Nts Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-pMecp2-SaCas9-pU6-sgRNA

   Plasmid

   #159173

   Purpose

   Vector A encodes pAAV-pMecp2-SaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of SaCas9-sgRNA in neurons

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   SaCas9
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pMecp2

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. single polypeptide FPX biosensor for caspase-8

    Plasmid

    #60884

    Purpose

    FPX biosensor for caspase-8

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-8
    

    Use

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCMV-AAAAA-nCas9(D10A)-UGI

    Plasmid

    #187409

    Purpose

    DddA AAAAA variant

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA AAAAA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6-cGAS g2 Cas9-T2A-mCherry

    Plasmid

    #164253

    Purpose

    Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgcGAS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

    Plasmid

    #159912

    Purpose

    Mutagenesis of Kcnd3

    Depositor

    Larry Zweifel

    Insert

    Kcnd3 (Kcnd3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9

    Plasmid

    #186699

    Purpose

    Plasmid encoding PRDM9dC-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9 (PRDM9 Human)
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p2T-cmv-SpCas9-TadCBEa-BlastR

    Plasmid

    #193847

    Purpose

    Express TadCBEa in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadCBEa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOTTC1124 - pAAV MeCP2 SpCas9(D10A)

    Plasmid

    #112719

    Purpose

    An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    SpCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Mecp2

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover

    Plasmid

    #196829

    Purpose

    Express dpdpCas13b with AAAA mutation and Clover

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    dpspCas13b(AAAA)
    

    Use

    Tags

    3XFLAG and NES

    Expression

    Mammalian

    Mutation

    K367A, D369A and K370A

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

    Plasmid

    #126698

    Purpose

    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

    Insert

    sgRNA targeting human SCGB3A2 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CMV-FLEX-SaCas9-U6-sgVamp2

    Plasmid

    #159920

    Purpose

    Mutagenesis of Vamp2

    Depositor

    Larry Zweifel

    Insert

    Vamp2 (Vamp2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pC009 LshCas13a locus with targeting spacer

    Plasmid

    #91900

    Purpose

    LshCas13a locus into pACYC184 with targeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LshCas13a locus with targeting spacer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge