We narrowed to 30,515 results for: Ide

1. pDM1081

   Plasmid

   #108981

   Purpose

   Knockout cassette Hygromycin

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Insert

   Knockout cassette Hygromycin
   

   Use

   Cre/Lox; Dictyostelium discoideum knockout vector

   Tags

   Expression

   Mutation

   Promoter

   coaA

   Available since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRC319

   Plasmid

   #61272

   Purpose

   Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.

   Depositor

   Timothy Lu

   Article

   Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

   Insert

   Cas9, tracrRNA, crRNA
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMM764

   Plasmid

   #68901

   Purpose

   IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), constiutive NanoLuc, pNBU2 backbone, AmpR

   Depositor

   Christopher Voigt, Timothy Lu

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Inserts

   dCas9
   LacI
   sgRNA
   NanoLuc
   

   Use

   Tags

   Expression

   Mutation

   L51P

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMJ333: pU6-MmeFz2_omegaRNA-entry

   Plasmid

   #205267

   Purpose

   Human expression vector for MmeFz2 omegaRNA (BbsI guide entry site)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   MmeFz2 omegaRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMM733

   Plasmid

   #68898

   Purpose

   IPTG-inducible CRISPRi vector targeting NanoLuc (NL4), constiutive NanoLuc, pNBU2 backbone, AmpR

   Depositor

   Christopher Voigt, Timothy Lu

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Inserts

   dCas9
   LacI
   sgRNA
   NanoLuc
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. p5'Entry-lck-5.5kb

   Plasmid

   #58890

   Purpose

   provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes

   Depositor

   Jeffrey Yoder

   Insert

   zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
   

   Use

   Gateway 5' entry clone

   Tags

   Expression

   Mutation

   mutated single nucleotide (T-->C, 13th nucleot…

   Promoter

   lymphocyte-specific protein tyrosine kinase (lck)…

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMJ106: pRS426-His-MBP-NlovFz2_Native

   Plasmid

   #205253

   Purpose

   S. cerevisiae expression vector for NlovFz2 native guide

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   NlovFz2
   

   Use

   Tags

   10xHis, MBP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMJ45: pRS426-His-MBP-SpuFz1_Native

   Plasmid

   #205252

   Purpose

   S. cerevisiae expression vector for SpuFz1 native guide

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Use

   Tags

   10xHis, MBP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMJ332: pU6-GtFz1_omegaRNA-entry

   Plasmid

   #205265

   Purpose

   Human expression vector for GtFz1 omegaRNA (BbsI guide entry site)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   GtFz1 omegaRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMJ123: pRS426-His-MBP-SpuFz1_PSP1

    Plasmid

    #205256

    Purpose

    S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMM710

    Plasmid

    #68893

    Purpose

    IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), pNBU2 backbone, AmpR

    Depositor

    Christopher Voigt, Timothy Lu

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-SdrG_N2N3-HIS-ybbr

    Plasmid

    #101238

    Purpose

    SdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    SdrG_N2N3
    

    Use

    Tags

    H3V 3C, HIS, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMJ162: pU6-SpuFz1_omegaRNA(75nt)-entry

    Plasmid

    #205261

    Purpose

    Human expression vector for SpuFz1 omegaRNA 75-nt scaffold (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMJ240: pRS426-His-MBP-MmeFz2_Native

    Plasmid

    #205255

    Purpose

    S. cerevisiae expression vector for MmeFz2 native guide

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    MmeFz2
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDM1047

    Plasmid

    #108990

    Purpose

    Inducible expression vector (dox-on), N-terminal GFP tagging

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Dictyostelium discoideum inducible expression vec…

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMJ235: pRS426-His-MBP-GtFz1_Native

    Plasmid

    #205254

    Purpose

    S. cerevisiae expression vector for GtFz1 native guide

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    GtFz1
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDM1209

    Plasmid

    #108986

    Purpose

    extrachromosomal expression vector, C-terminal GFP tagging

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Dictyostelium discoideum expression vector

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX2TK-Osh2-PH

    Plasmid

    #71370

    Purpose

    bacterial expression of PH domain for phosphoinositide binding assay

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Osh2-PH
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 277-398

    Promoter

    tac

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pDM1079

    Plasmid

    #108979

    Purpose

    Knockout cassette Blasticidin

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    Knockout cassette Blasticidin
    

    Use

    Cre/Lox; Dictyostelium discoideum knockout vector

    Tags

    Expression

    Mutation

    Promoter

    coaA

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDM1046

    Plasmid

    #108989

    Purpose

    Inducible expression vector (dox-on), N-terminal mCherry tagging

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Dictyostelium discoideum inducible expression vec…

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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