We narrowed to 19,001 results for: Tec

1. pMS22H

   Plasmid

   #133904

   Purpose

   Expression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWH

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTK101

   Plasmid

   #59561

   Purpose

   SIN SP6 P1234 (nsP2 P726S) SGP(14) mKate-G8-L7Ae-PEST 4xFF4

   Depositor

   Ron Weiss

   Article

   Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

   Insert

   SGP(14) mKate-G8-L7Ae-PEST 4xFF4
   

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBW829_pCAG-pExpr-LUT

   Plasmid

   #87554

   Purpose

   Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   EGFP
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. YSBE 559 : pNAT_6xHA

   Plasmid

   #92087

   Purpose

   Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, NAT selection

   Depositor

   Yong-Sun Bahn

   Article

   So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

   Insert

   6xHA
   

   Use

   tagging proteins in cryptococcus neoforman

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFLIP38

   Plasmid

   #65616

   Purpose

   FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Tags

   6His-edCit-attR1 and attR2-edCer-cMyc

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Id2-Halo donor

   Plasmid

   #113116

   Purpose

   Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Id2 donor region including homology arms and HaloTag
   

   Use

   Synthetic Biology

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIp211-P30-His-POL30-3'

   Plasmid

   #99546

   Purpose

   Yeast integrative vector for expression of His-tagged POL30 (PCNA); URA3 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   POL30 (POL30 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Yeast

   Promoter

   POL30

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSET FLIPE-1m

   Plasmid

   #13540

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207W
   

   Tags

   ECFP and Venus

   Expression

   Bacterial

   Mutation

   A207W in binding protein

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. GGE252

   Plasmid

   #120791

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsDown_GSY-NotI
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. GGE253

    Plasmid

    #120790

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsUp_GSY-NotI
    

    Use

    Cloning/assembly plasmid

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. 7xNLS-pMJ915v2-sfGFP

    Plasmid

    #88922

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-TetR-Rad18UBD

    Plasmid

    #119010

    Purpose

    Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    TetR
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19/mlcR25-mEGFP

    Plasmid

    #154295

    Purpose

    Fluorescent reporter for conony screening without an inducer (e.g. IPTG); TED module (Plac-lacO-mlcR25-mEGFP)

    Depositor

    Shigehiko Yumura

    Article

    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

    Insert

    25-bp fragment of the 3′ end of mlcR (mlcR25); mEGFP
    

    Expression

    Bacterial

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pST1374-Lb-Cpf1-NLS

    Plasmid

    #107680

    Purpose

    Expresses LbCpf1 in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    LbCpf1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCellFree_G03 S100A1

    Plasmid

    #67048

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    S100A1 (S100A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. mEos2-VASP-5

    Plasmid

    #57433

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    VASP
    

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCfB4132

    Plasmid

    #106127

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. JME4393

    Plasmid

    #129657

    Purpose

    LYS5ex_CrisprCas9-yl_RFP: CAS9 vector with LYS5ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFLIP43

    Plasmid

    #65618

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Tags

    6His-edAFPt9-attR1 and attR2-edCer-cMyc

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLR5-CAG-NLS-tdiRFP670

    Plasmid

    #82621

    Purpose

    Expresses NLS-tdiRFP670 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Insert

    NLS-tdiRFP670
    

    Use

    Piggybac vector

    Expression

    Mammalian

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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