We narrowed to 23,577 results for: CHI

1. pJK03_Rho_basal_DsRed

   Plasmid

   #173490

   Purpose

   Vector backbone used to clone MPRA libraries

   Depositor

   Barak Cohen

   Article

   White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

   Insert

   Bovine Rhodopsin promoter driving DsRed fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Bovine Rhodopsin

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pME-nlsGal80(opt)

   Plasmid

   #51618

   Purpose

   vertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stop

   Depositor

   Joshua Bonkowsky

   Article

   Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

   Insert

   Gal80(opt) (GAL80 Budding Yeast)
   

   Use

   Vertebrate expression

   Tags

   NLS

   Expression

   Mutation

   codon optimized

   Promoter

   Available since

   May 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AB.pCCL.sin.cPPT.GFP.miR-185-5p.sensor.PGK.dNGFR.WPRE

   Plasmid

   #85930

   Purpose

   Sensor Plasmid

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-185-5p Sensor (MIR185 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDEST-hPDIb′a′

   Plasmid

   #60556

   Purpose

   enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

   Depositor

   Han Choe

   Article

   Song et al Appl Biochem Biotechnol. 2013 May;170(1)

   Type

   Empty backbone

   Use

   Tags

   His6-hPDIb'a' tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGBW-m4046942

   Plasmid

   #145757

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp6

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pCfB8792

   Plasmid

   #126910

   Purpose

   Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   ADE2 gRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNOC-CRISPR-HA

   Plasmid

   #99370

   Purpose

   Expresses Cas9-HA and sgRNA. Hygromycin resistance.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   Cas9-HA

   Expression

   Mutation

   Promoter

   Ribi promoter

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGBW-m4046763

   Plasmid

   #145606

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pRK5-Merlin-Flag-HA

   Plasmid

   #27103

   Depositor

   Filippo Giancotti

   Article

   Li et al Cell. 2010 Feb 19. 140(4):477-90.

   Insert

   Merlin (Nf2 Mouse)
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pCTCON2-GYB

    Plasmid

    #160927

    Purpose

    Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Inserts

    BFP
    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFETCh_NFIA_iso1

    Plasmid

    #104039

    Purpose

    Donor vector for 3' FLAG tag of human NFIA_iso1

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    NFIA_iso1 homology arms (NFIA Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NAX_CAGGS_CAS9_EBFP2

    Plasmid

    #167857

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPR_eGFP_314

    Plasmid

    #111822

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 294-314
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBlue-loxPuro-APEX-PML_LASA

    Plasmid

    #140273

    Purpose

    a targeting plasmid for APEX-PML

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    APEX2
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    endogenous

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA-LEU2-I-SceI

    Plasmid

    #67595

    Purpose

    I-SceI expression regulated by tetracycline inducible system in S. pombe

    Depositor

    Kurt Runge

    Article

    Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

    Inserts

    nmt terminator
    I-SceI
    TetR
    

    Use

    Gene targeting in yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSecEffector

    Plasmid

    #172891

    Purpose

    Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901

    Depositor

    Urartu Seker

    Article

    Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

    Inserts

    Bxb1
    TP901
    TEV Display cassette
    ProD promoter between anti aligned Bxb1 sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ProD, nTETO, and pBAD /AraC

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNC1136

    Plasmid

    #41562

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-FUS1-UBIYdkGFP*-SpHis5-Tim9
    

    Use

    Tags

    Expression

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti_CMV:Flag-CeNLuc_SV40:PuroR

    Plasmid

    #183036

    Purpose

    Expresses the FLAG-tagged CeNLuc (Blue LumiFluor) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    CeNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAL612

    Plasmid

    #119860

    Purpose

    precusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 Cysteine

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    outer membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Cysterine 290 to Serine and Glycine 244 to Cystei…

    Promoter

    lac

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLEX_970_puro_DEST_YFP

    Plasmid

    #45295

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    YFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

21. GFP-WIPI2d-TEVcs-TSF

    Plasmid

    #171420

    Purpose

    For the purifcation of GFP- WIPI2d protein

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pETM41-EcPPXc

    Plasmid

    #38329

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    E. coli polyphosphatase (ppx Escherichia coli)
    

    Use

    Tags

    His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    C-terminus of E. coli polyphosphatase (described …

    Promoter

    T7

    Available since

    Aug. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

23. 3XFlag DVL2 (∂PY)

    Plasmid

    #24808

    Depositor

    Jeff Wrana

    Article

    Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

    Insert

    DVL2 (DVL2 Human)
    

    Use

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Deletion of the PY motif: PPPY

    Promoter

    Available since

    June 22, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

24. 3xFNLDD/pMXs-IG

    Plasmid

    #48972

    Purpose

    Expresses 3xFNLDD in mammalian cells

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2015 Jan 21;10(1):e0116579. do

    Insert

    3xFNLDD
    

    Use

    Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGAGmEos2-N1

    Plasmid

    #46372

    Depositor

    Suliana Manley

    Article

    Gunzenhauser et al Nano Lett. 2012 Sep 12;12(9):4705-10. do

    Insert

    Gag
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Rev independentHXB2 Gag sequence

    Promoter

    CMV

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRS416-Aga1p-FAPB2.3.6

    Plasmid

    #158147

    Purpose

    Yeast display reporter for non-display yeast strains (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    Aga1p-FAPB2.3.6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PX458_NFIA_iso1_2

    Plasmid

    #104049

    Purpose

    Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    NFIA_iso1 gRNA (NFIA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PX458_NFIA_iso1_1

    Plasmid

    #104048

    Purpose

    Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    NFIA_iso1 gRNA (NFIA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCfB5191

    Plasmid

    #126911

    Purpose

    Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    X-4 and XII-5 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGBW-m4046933

    Plasmid

    #145592

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. Nano-lantern(Ca2+)_600/pcDNA3

    Plasmid

    #51982

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-2G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pKM263-EcPPXc

    Plasmid

    #38328

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    E. coli polyphosphatase (ppx Escherichia coli)
    

    Use

    Tags

    6xHIS, GST, and TEV

    Expression

    Bacterial

    Mutation

    C-terminus of E. coli polyphosphatase (described …

    Promoter

    T7

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. E17TAG GFP zeo resistance

    Plasmid

    #69118

    Purpose

    For E17TAG GFP reporter expression in non-zeocin resistant strains

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    E17TAG GFP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E17 to TAG

    Promoter

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pGBW-m4136463

    Plasmid

    #149527

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pZA31

    Plasmid

    #158509

    Purpose

    In planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-4xMyc. Km plant selection marker.

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1-4xMyc
    

    Use

    Tags

    4xMyc

    Expression

    Plant

    Mutation

    L17E

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFIN-EF1a-GFP-WPRE

    Plasmid

    #44352

    Depositor

    Susan Semple-Rowland

    Article

    Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

    Insert

    EF1a-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    human elongation factor -1a (pos 2526-3969)

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGBW-m4046764

    Plasmid

    #145673

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. fADL-1e-flag-linker-p3

    Plasmid

    #139441

    Purpose

    For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.

    Depositor

    Alexander Gabibov

    Article

    Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

    Insert

    linker with pelB-leader, cloning site, flag-tag and p3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. L1-A426D

    Plasmid

    #15485

    Depositor

    Vance Lemmon

    Article

    Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

    Insert

    L1-A426D (L1CAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Alanine 426 changed to Aspartic Acid

    Promoter

    Available since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

40. pFETCh_NFIL3

    Plasmid

    #86287

    Purpose

    Donor vector for 3' FLAG tag of human NFIL3

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    NFIL3 homology arms (NFIL3 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. PX458_NFIL3_2

    Plasmid

    #86349

    Purpose

    Encodes gRNA for 3' target of human NFIL3

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against NFIL3 (NFIL3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. PX458_NFIL3_1

    Plasmid

    #86348

    Purpose

    Encodes gRNA for 3' target of human NFIL3

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against NFIL3 (NFIL3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. AB.pCCL.sin.cPPT.GFP.miR-21-3p.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85857

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-21-3p Sensor (MIR21 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSA10(gfcB-His-CD)

    Plasmid

    #176194

    Purpose

    expresses 3 overlapping genes (gfcB-6xHis, gfcC, gfcD) from this one operon

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcBCD
    

    Use

    Tags

    GfcB-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pBH31(gfcD)

    Plasmid

    #176199

    Purpose

    Encodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasm

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcD
    

    Use

    Tags

    pelC leader-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. pBH31(gfcD-10xHis)

    Plasmid

    #176200

    Purpose

    Encodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasm

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcD
    

    Use

    Tags

    pelC leader-10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAID-CMV linearizing in pX330

    Plasmid

    #140609

    Purpose

    Crispr/Cas9 plasmid for linearization of pAID-CMV

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    sgRNA for pAID-EF1a plasmid digestion
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pAL725

    Plasmid

    #119862

    Purpose

    precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Leucine 244 to Cysteine, Aspartate 287 to Cysteine

    Promoter

    T5

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. p2T-CMV-evoAmax-BlastR

    Plasmid

    #152996

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    evoA-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pC0.167

    Plasmid

    #119636

    Purpose

    Level 0 Part. Insertion site

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    6803 NS2 Up Flank
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    non functional/large non-specific insertion

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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