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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 18,730 results for: IRE

Showing: 9151 - 9200 of 18730 results

  1. NmMetQ

    Plasmid
    #172111
    Purpose
    Expression of Methionine Binding Protein (MetQ) with signal sequence
    Depositor
    Douglas Rees
    Article
    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:
    Insert
    Methionine Binding Protein
    Use
    Tags
    10x Histidine Tag and N.meningitidis signal seque…
    Expression
    Bacterial
    Mutation
    Changed Proline 22 to Alanine
    Promoter
    T7 Promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172111-plasmid-map-sequence-id-344824

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  2. pColE1-AlphaOmega

    Plasmid
    #165867
    Purpose
    Terminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    165867-plasmid-map-sequence-id-345193

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  8. pET21-pelB-LaG-3

    Plasmid
    #172740
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-3
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172740-plasmid-map-sequence-id-340191

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  45. pUE1-TSP-ORF68 K450A/R451A

    Plasmid
    #162653
    Purpose
    Expresses ORF68 K450A/R451A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1
    Insert
    ORF68
    Use
    Tags
    N-terminal 2xStrep-Tag II and HRV 3C site
    Expression
    Mammalian
    Mutation
    Amino acids K450 and R451 mutated to alanine
    Promoter
    CMV
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    162653-plasmid-map-sequence-id-320644

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  47. Donor-PIGAmut

    Plasmid
    #153548
    Purpose
    Used as a donor vector for the targeted knock-in of a mutation in PIGA exon 6
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6)
    Use
    Donor plasmid
    Tags
    N/A
    Expression
    Mutation
    a 3-bp mutation in exon 6
    Promoter
    No promoter
    Available since
    Dec. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    153548-plasmid-map-sequence-id-319288

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Showing: 9151 - 9200 of 18730 results