We narrowed to 9,373 results for: Pol;
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Plasmid#58716PurposeExpresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1SDepositorInsertNotexin
TagsMethionineExpressionBacterialMutationChanged Asparagine 1 to SerinePromoterT7Available SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SYN-H-sDarken
Plasmid#184797PurposeHigh affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.DepositorInsertH-sDarken
UseAAVExpressionMammalianPromoterhSynAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEf1a OsTIR ires NEO pA T2BH
Plasmid#127910PurposeTransposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoterDepositorInsertOsTIR
UseTransposonAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1-eIF4E K119A
Plasmid#199440PurposeBacterial expression plasmids for production of recombinant GST-eIF4E K119ADepositorInsert1977 (EIF4E Human)
TagsGST-fusion proteinExpressionBacterialMutationchanged lysine 119 to alanineAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-Hyg-Dest-mCherry-PAR-6B
Plasmid#194908PurposeTo generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.DepositorInsertPAR-6B (Pard6b Mouse)
UseMouse TargetingTagsmCherryExpressionMammalianMutation39 C to G, silent mutationAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hTGM3-Flag
Plasmid#180408Purposelentiviral transduction of human TGM3 (with a C-terminal Flag tag) geneDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330 Human 3' HP1a gRNA
Plasmid#127907PurposeWT Cas9 Vector targeting the 3' end of the human HP1a geneDepositorInsertgRNA for Human 3' HP1a
UseCRISPRAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-Cre-2A-mCherry-FKF
Plasmid#119868PurposeDonor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertNLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
TagsmCherryExpressionBacterialAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET19-MTdTwt
Plasmid#207460PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT)DepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDT386
Plasmid#174733PurposeExpresses His-tagged DT386, a truncated diphtheria toxin without a receptor-binding domain.DepositorInsertDT386
TagsHistagExpressionBacterialMutationDeleted amino acids 412-560 (the receptor binding…PromoterT5Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT RAD23B
Plasmid#201439PurposeExpresses human RAD23B with an N-terminal FLAG tag in mammalian cellsDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-mycRSK2wt
Plasmid#15827DepositorInsertRibosomal S6 kinase 2 (Rps6ka3 Mouse)
UseRetroviralTagsmycExpressionMammalianMutationwild typeAvailable SinceNov. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
CMV-Tagger
Plasmid#129396PurposeExpresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoterDepositorInsertrpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2
TagsFLAG, HA, and V5ExpressionMammalianPromoterCMVAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCWXPG-UBI-MafA
Plasmid#114302PurposeConstitutive expression of the MafA trancription factorDepositorInsertMafA (MAFA Human)
UseLentiviralAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-shRNA_Tet3
Plasmid#85740PurposeshRNA against mouse Tet3DepositorAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hTGM4-Flag
Plasmid#180409Purposelentiviral transduction of human TGM4 (with a C-terminal Flag tag) geneDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hTGM5-V5
Plasmid#180414Purposelentiviral transduction of human TGM5 (with a C-terminal V5 tag) geneDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SYN-L-sDarken
Plasmid#184798PurposeLow affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.DepositorInsertL-sDarken
UseAAVExpressionMammalianPromoterhSynAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pScalps_puro_mIL-2Rα
Plasmid#59917PurposeExpresses mouse interleukin-2 receptor alpha chainDepositorInsertinterleukin 2 receptor alpha (Il2ra Mouse)
UseLentiviralAvailable SinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-OF (mutant TCF-4 sites)
Plasmid#16559DepositorInsertTCF-4 binding sites (TCF4 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutation3 copies of mutant Tcf-4 binding sitesAvailable SinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSCALPs-HA-NFAT1 (4-460)-GFP
Plasmid#88879PurposeLentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFPDepositorInsertHA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
UseLentiviralTagsGFP fusion protein and HA tagExpressionMammalianMutationamino acids 4-460 only; L78P & L287P *see bel…PromoterSFFV (spleen focus forming virus)Available SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIA1385
Plasmid#166862PurposeCodon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.DepositorAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGM8
Plasmid#220166PurposeExpresses a reporter for an alpha-helical OMM proteinDepositorAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX Flag RagC S75N
Plasmid#112757Purposeexpress Flag-RagC (S75N mutant)DepositorAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
cyclin D1-HA-pBabe-Hygro
Plasmid#22703DepositorAvailable SinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
LLP109 pEF1a-gRNA-GFP
Plasmid#100935PurposeGFP, puromycin and gRNA scaffold driven by EF1a promoterDepositorInsertgRNA scaffold
UseGrna scaffoldExpressionMammalianAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
hsp70: mKate2-DN Kif5a
Plasmid#105972Purposeheat shock inducible transgenesisDepositorAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTon1(acgfp1)
Plasmid#233648PurposeExpression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.DepositorInserttarget sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
UseAmphibian expression, tet-onAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNG1256
Plasmid#149710PurposeExpresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complexDepositorAvailable SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
CIBN-GFP-TMD
Plasmid#79571Purposeexpression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)DepositorInsertsyntaxin 1A (Stx1a Mouse)
TagsCIBN and EGFPExpressionMammalianMutationaa 249_288 (C-terminal region (polybasic stretch …PromoterCMVAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only