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We narrowed to 63,044 results for: cloning

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Showing: 3681 - 3700 of 63044 results

  6. pcDNA C12 ecDHFR DD YFP HA

    Plasmid
    #192815
    Purpose
    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.
    Depositor
    John Hulleman
    Publication
    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021
    Insert
    E. coli dihydrofolate reductase
    Tags
    enhanced yellow fluorescent protein and hemagglut…
    Expression
    Mammalian
    Mutation
    W74R/T113S/E120D/Q146L
    Promoter
    CMV
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192815-plasmid-map-sequence-id-377240

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  9. pHN781

    Plasmid
    #178828
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Tags
    His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178828-plasmid-map-sequence-id-351929

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  11. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid
    #170977
    Purpose
    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.
    Depositor
    Miki Ebisuya
    Publication
    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:
    Insert
    CShroom3 (Shroom3 Mouse)
    Use
    Gateway Cloning
    Tags
    SspB and mCherry
    Mutation
    Isoform X2, deleted aminoacids 1 - 1388
    Available since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170977-plasmid-map-sequence-id-336791

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  17. CIDAR MoClo Extension, Volume I

    Plasmid kit
    #1000000161
    Purpose
    Plasmids to efficiently build a wide variety of gene expression constructs. Can be used as a stand-alone toolkit or with the CIDAR MoClo Parts Kit (Addgene #1000000059).
    Depositor
    Richard Murray
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only

  20. pLenti-H2B-iRFP720

    Plasmid
    #128961
    Purpose
    Lentiviral plasmid for expression of far red iRFP720 nuclear marker
    Depositor
    Carlos Carmona-Fontaine
    Insert
    Human histone H2B (H2BC21 Human)
    Use
    Lentiviral
    Tags
    iRFP720
    Expression
    Mammalian
    Promoter
    hPGK
    Available since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    128961-plasmid-map-sequence-id-252385

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Showing: 3681 - 3700 of 63044 results