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CSII-IDR2-owlTRIMCyp-FOS K283D Q287D

Plasmid

#79068

Purpose

Express owl monkey TRIMCyp K283D, Q287D in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5Cyp (owl monkey) (K283D, Q287D)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Changed Lys 283 to Asp and Gln 287 to Asp

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIMCyp (owl monkey)

Plasmid

#79035

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5Cyp (owl monkey)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)

Plasmid

#79030

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

deleted amino acid 294-515 from frican green monk…

Promoter

polyhedrin

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hATG5 WT

Plasmid

#111803

Purpose

Mammalian Expression of hATG5

Depositor

Jun Hee Lee

Article

Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

Insert

hATG5 (ATG5 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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Integrin β4-GFP

Plasmid

#205092

Purpose

Express GFP-tagged human integrin β4 in mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

Integrin beta 4 (ITGB4 Human)

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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Integrin β8-GFP

Plasmid

#205093

Purpose

Express GFP-tagged human integrin β8 in mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

Integrin beta 8 (ITGB8 Human)

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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GFP1-10-miniCMV-GFP11×11-GFP-tDeg

Plasmid

#185404

Purpose

Expresses GFP1-10 and GFP11×11-GFP-tDeg

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

GFP1-10

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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3xflag-APEX2-LRRK2

Plasmid

#164622

Purpose

Expresses LRRK2 tagged with APEX2 and 3xFLAG

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

LRRK2 (LRRK2 Human)

Tags

3xFLAG and APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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GA-DEVD-GB-NES

Plasmid

#61020

Purpose

green ddFP based caspase-3 biosensor

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP A and ddGFP B

Tags

HindIII and NES sequence LALKLAGLDIGS placed afte…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pXJ-His-CD276

Plasmid

#232662

Purpose

transient expresses CD276 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CD276 (CD276 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_Ado1.0mut

Plasmid

#153290

Purpose

Express the adenosine-insensitive control sensor GRAB_Ado1.0mut in astrocytes

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)

Use

AAV

Promoter

GfaABC1D

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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EID1-pGex-2TK

Plasmid

#22689

Depositor

William Kaelin

Article

Miyake et al Mol Cell Biol. 2000 Dec . 20(23):8889-90

Insert

E1A-like inhibitor of differentiation 1 (EID1 Human)

Tags

GST

Expression

Bacterial

Mutation

wt cDNA

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pCG-TPMV Fd32

Plasmid

#163964

Purpose

Expression of TPMV F glycoprotein in mammalian cells

Depositor

Jakob Reiser

Article

Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

Insert

TPMV F glycoprotein

Expression

Mammalian

Mutation

Lacking 32 amino acids at C-terminus

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX307 RFP

Plasmid

#163976

Purpose

Mammalian expression vector encoding RFP (for example, to normalize plasmid-based protein expression)

Depositor

Daniel Bachovchin

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

RFP

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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LDB-3-p65

Plasmid

#158984

Purpose

Expresses LDB-3 fused with a C-terminal p65 transcriptional activation domain in mammalian cells

Depositor

Liangcai Gu

Article

Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

Insert

LDB-3-p65

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Cyto_K0

Plasmid

#133864

Purpose

Expression of cytoplasmic Ribosome non-stalling reporter

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

Cyto_K0

Tags

EGFP and RFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9-dMQ1-EGFG

Plasmid

#89794

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR and Lentiviral

Tags

3*FLAG and T2A-EGFP

Expression

Mammalian

Mutation

C141S, TGC-->TCC; S317A, AGC-->GCC

Promoter

hUbC

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pJKW 0690

Plasmid

#74123

Purpose

Photinus pyralis sulfotransferase ST3 - pHis8-4

Depositor

Jing-Ke Weng

Article

Fallon et al Biochemistry. 2016 May 26.

Insert

Photinus pyralis sulfotransferase 3

Tags

8x-His,TEV protease site (ENLYFQ)

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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CLTA-mCherry HDRT Source (pTR 177)

Plasmid

#112017

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-mCherry HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-XK

Plasmid

#184369

Purpose

Express human XK in Expi293 cells

Depositor

Karin Reinisch

Article

Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

Insert

Human XK (XK Human)

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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