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pHcRed-NPM1wt-C1

Plasmid

#131818

Purpose

Expresses HcRed-NPM1wildtype (human) in mammalian cells through transient transfection

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Tags

HcRed

Expression

Mammalian

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pJAB988

Plasmid

#133920

Purpose

miniICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoter

Depositor

Christopher Voigt

Article

Brophy et al Nat Microbiol. 2018 Sep;3(9):1043-1053.

Insert

miniICE-tet(M)cat(PC194)_PT7-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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ampl43

Plasmid

#161830

Purpose

pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

Depositor

Saeed Tavazoie

Article

Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

TEF1

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b_EcSSB_1

Plasmid

#78203

Purpose

Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

Depositor

Martin Webb

Article

Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

Insert

ssb (G26C)

Expression

Bacterial

Mutation

G26C (please see depositor comment below)

Promoter

T7

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)

Plasmid

#110559

Purpose

miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3/HA-FLAG-RBP2

Plasmid

#14800

Depositor

William Kaelin

Article

Klose et al Cell. 2007 Mar 9. 128(5):889-900.

Insert

Retinoblastoma Binding Protein 2 (KDM5A Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TetON-Puro-HIF1a 3809

Plasmid

#118705

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pGL2-NOS2Promoter-Luciferase

Plasmid

#19296

Depositor

Charles Lowenstein

Article

Lowenstein et al Proc Natl Acad Sci U S A. 1993 Oct 15. 9

Insert

NOS2 Promoter (Nos2 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-mC-G-LC3B-P

Plasmid

#124974

Purpose

evaluate autophagy

Depositor

Kazuhiro Oka

Article

Sutton et al Autophagy. 2018;14(4):637-653. doi: 10.1

Insert

mCherry-GFP-LC3B

Use

Lentiviral

Promoter

CMV

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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FKBP-myc

Plasmid

#20211

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FKBP (FKBP1A Human)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pET3a-hUb

Plasmid

#61937

Purpose

Expression of hUb in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

human ubiquitin (UBC Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pCMV6-AC 4-μHOM

Plasmid

#113619

Purpose

Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

4-μHOM variant of EGFP

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-3m160-HilA

Plasmid

#153325

Purpose

Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-3m160 DARPin

Use

Salmonella expression

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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NLS-HA-tdMCP-tdTomato

Plasmid

#183938

Purpose

tandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tag

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdMCP-tdTomato (cp Synthetic)

Tags

NLS-HA

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(7FTrp)RS

Plasmid

#177310

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al ACS Sens. 2022 Jan 10. doi: 10.1021/acss

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Lapronic/pcDNA3.1(-)

Plasmid

#140756

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Lapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)

Expression

Mammalian

Promoter

CMV

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)

Plasmid

#110558

Purpose

miniTn7 delivery plasmid with lacIq-Ptac inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

lacIq-Ptac

Expression

Bacterial

Promoter

lacIq-Ptac

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-JEDI-1P-Kv-WPRE

Plasmid

#202612

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P-Kv

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

Plasmid

#84246

Purpose

Expresses direct fusion VPR-Sa dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sa dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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Profiline

Plasmid

#89925

Purpose

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin

Depositor

Antoine Jégou, Guillaume Romet-Lemonne

Article

Jegou et al PLoS Biol. 2011 Sep;9(9):e1001161. doi:

Insert

profiline-1 (PFN1 Human)

Tags

None

Expression

Bacterial

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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