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SAC1deltaTMD-FKBP-mCherry

Plasmid

#108123

Purpose

Recruitable cytosolic SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

SACM1L(1-520):FKBP1A(3-108):mCherry (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-520)

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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p7130 pHAGE-P-CMVt-N-HA-GAW-KIF20A

Plasmid

#100156

Purpose

Expresses KIF20A

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

KIF20A (KIF20A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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YEp181-CUP1-His-Ubi

Plasmid

#99539

Purpose

Yeast episomal vector for expression of His-tagged ubiquitin; LEU2 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

UBI3(1-76) (RPL40A Budding Yeast)

Tags

7xHis

Expression

Yeast

Promoter

CUP1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_HuD

Plasmid

#65757

Purpose

expresses FLAGHA-tagged HuD in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL4 (ELAVL4 Human)

Tags

FLAG HA and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pLPCX-PECAMTL

Plasmid

#45851

Depositor

Martin Schwartz

Article

Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

Insert

PECAM1 (PECAM1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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p6641 MSCV-P C-FlagHA 18E7-Kozak

Plasmid

#35019

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV18 E7 (E7 HPV 18)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

March 14, 2012

Availability

Academic Institutions and Nonprofits only
His-tagged-SF1

Plasmid

#28182

Depositor

Bernard Schimmer

Article

Frigeri et al Mol Endocrinol. 2000 Apr . 14(4):535-44.

Insert

SF1 (Nr5a1 Mouse)

Tags

His

Expression

Mammalian

Available Since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
FLAG-MED7

Plasmid

#15416

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED7 (MED7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B8-TEF(272)

Plasmid

#44376

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(272) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(272)

Available Since

April 16, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCV-Hygromycin-TTF-1

Plasmid

#119173

Purpose

Retroviral mammalian expression vector containing wt-human TTF-1 cDNA

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

NKX2-1 (NKX2-1 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup88

Plasmid

#87338

Purpose

To express human Nup88 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP88 (NUP88 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAC-GGPPipi

Plasmid

#53280

Purpose

Contains crtE and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces geranylgeranyl diphosphate in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtE, idi

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (4x TBE2 - mut)

Plasmid

#16600

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (4xTBE2 mut) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

mutant TBE2 sites

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
c-myc promoter (Frag D)

Plasmid

#16608

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Frag D) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

FragD (PvuII->SmaI). The PvuII site is at -484…

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
FLAG-MED21

Plasmid

#15422

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED21 (MED21 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pET28a-SARS-CoV-2 NSP13

Plasmid

#176492

Purpose

Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

Depositor

Xiaodong Cheng

Article

White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

Insert

SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)

Tags

His6 and T7

Expression

Bacterial

Mutation

I570 from SARS-CoV NSP13 was mutated to V to conv…

Promoter

T7

Available Since

Jan. 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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GLuc-FLAG-CB1

Plasmid

#158066

Purpose

Membrane expression detection of Cannabinoid receptor 1

Depositor

Adolfo Rivero-Muller

Article

Rodriguez-Rodriguez et al Sci Rep. 2020 Oct 23;10(1):18191. doi: 1

Insert

CB1 (CNR1 Human)

Use

AAV

Tags

Gaussia Luciferase

Expression

Mammalian

Mutation

contains 4Kb of 3' UTR

Promoter

pCAG

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBW2426_pCAG-PV1-iCre-N270-L1-GID1-NLS-BGHpA

Plasmid

#108729

Purpose

Expresses N-terminus to aa270 of Cre recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa270

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FGF1-4X

Plasmid

#120282

Purpose

Produce human recombinant FGF1-4X in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Basic fibroblast growth factor 1 mutant

Tags

6x His tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAM4787

Plasmid

#120088

Purpose

Cyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).

Depositor

Susan Golden

Article

Chen et al Microbiology. 2016 Dec;162(12):2029-2041

Insert

Plasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.

Use

Other

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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