We narrowed to 9,747 results for: ada

1. pcDNA3-ALK5 K232R

   Plasmid

   #80878

   Purpose

   mammalian expression of ALK5 K232R

   Depositor

   Aristidis Moustakas

   Article

   Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

   Insert

   ALK5 (TGFBR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K232R (kinase dead)

   Promoter

   CMV

   Available since

   Aug. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. MAC_N_IRF5

   Plasmid

   #167762

   Purpose

   Expresses IRF5 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   IRF5 (IRF5 Human)
   

   Use

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-CLCa

   Plasmid

   #196912

   Purpose

   Visualization of clathrin-coated pit formation in living mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Sakaushi et al Biosci Biotechnol Biochem. 2007 Feb;71(2

   Insert

   Clathrin-Coated light chain
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAC_N_GCM1

   Plasmid

   #167759

   Purpose

   Expresses GCM1 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   GCM1 (GCM1 Human)
   

   Use

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-MR1

   Plasmid

   #178603

   Purpose

   Mammalian expression of myc-tagged MR1

   Depositor

   Erik Procko

   Article

   McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

   Insert

   MR1 (MR1 Human)
   

   Use

   Tags

   Extracellular c-myc epitope ta and Signal peptide…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHLmMBP-1

   Plasmid

   #72343

   Purpose

   Mammalian expression of secreted mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Use

   Tags

   6His-tag, HA-tag, Strep-tag II, and mMBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUWR-Cad-Venus

   Plasmid

   #58328

   Purpose

   Destination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genome

   Depositor

   Denise Montell

   Article

   Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

   Insert

   Cad-Venus
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   poly-ubiquitin

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pHLP700

   Plasmid

   #203742

   Purpose

   C-terminal tagging of target proteins with AID*/3xHA degron

   Depositor

   Mark Hall

   Article

   Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

   Insert

   IAA17 auxin binding domain codons 71-114
   

   Use

   Pcr amplification template for gene tagging

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHLP701

   Plasmid

   #203743

   Purpose

   C-terminal tagging of target proteins with 3xV5-IAA17 degron

   Depositor

   Mark Hall

   Article

   Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

   Insert

   IAA17 auxin binding domain
   

   Use

   Pcr amplification template for gene tagging

   Tags

   3xV5

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

    Plasmid

    #159803

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pACT2-μ3A

    Plasmid

    #198174

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-3 μ3A
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    silent substitution in codon 225

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACT2-μ3B

    Plasmid

    #198175

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-3 μ3B
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-N2-RIPK3

    Plasmid

    #78822

    Purpose

    To detect localization of RIPK3 protein in live mammalian cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pRS315e_pGal-nCas9(D10A)-PmCDA1

    Plasmid

    #79617

    Purpose

    Expresses nCas9(D10A)-PmCDA1 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Yeast

    Mutation

    D10A for nickase Cas9

    Promoter

    pGal1

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAG

    Plasmid

    #86871

    Purpose

    To express SF3B6 delete NLS signal with a GFP and FLAG tags at N- and C-terminus, respectively.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    SF3B6 (SF3B6 Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    amino acids 7-29 deletion

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. RalGDS-YPet

    Plasmid

    #134929

    Purpose

    Fluorescently tagged affinity reagent

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    RaIGDS RBD, YPet
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p8103 LentiCRISPR v2 sgNT-2

    Plasmid

    #163313

    Purpose

    Expresses Cas9 and a non-targeting control guide RNA

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    sgNT-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-6011nes

    Plasmid

    #108652

    Purpose

    An ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-ERK-EV
    

    Use

    Tags

    XFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFUChW Parkin shRNA Mul1 shRNA

    Plasmid

    #78523

    Purpose

    Lentiviral vector encoding two shRNAs and mCherry reporter

    Depositor

    David Chan

    Article

    Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

    Insert

    Park2 shRNA, Mul1 shRNA (Prkn, Mul1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Orange Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71346

    Purpose

    Express Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern(Ca2+)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only