We narrowed to 34,882 results for: CaS;

1. 3xNLS-NLP-cMyc-cMyc enAspCas12a

   Plasmid

   #182127

   Purpose

   pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   3xNLS-NLP-cMyc-cMyc enAspCas12a
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AOI-WT-Cas9-sg-mouse Suz12-F2-GFP

   Plasmid

   #91882

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Suz12 (Suz12 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pZLCv2-gD4Z4-1-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106352

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   D4Z4 gRNA-1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

   Plasmid

   #81206

   Purpose

   CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   pGinB-8X(GGS)-dCas9-FLAG-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMD19T-psba1-Ppsba2-dCas9-SpR

   Plasmid

   #73220

   Purpose

   Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

   Depositor

   Paul Hudson

   Article

   Yao et al ACS Synth Biol. 2015 Dec 28.

   Insert

   dCas9 from S. pyogenes
   

   Use

   Tags

   c-myc

   Expression

   Bacterial

   Mutation

   Silent mutation at bp 1341 A->C to remove an E…

   Promoter

   PpsbA2

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX399 Mycoplasma gallisepticum str. F Cas9

   Plasmid

   #68335

   Purpose

   Mycoplasma gallisepticum str. F Cas9

   Depositor

   Feng Zhang

   Insert

   MgCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Construct 7 - CMVp-Cas9-3xNLS-HSVpA

   Plasmid

   #81247

   Purpose

   Expresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian cell expression

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   Cas9-3xNLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKEW321-MbCas12a-RFP-GFP-array

   Plasmid

   #115661

   Purpose

   Encodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   GFP and RFP array for MbCas12
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-IRES-GFP-bat-Caspase-4

   Plasmid

   #183377

   Purpose

   Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-4
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106355

    Purpose

    CRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locus

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    MYOD1 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xNLS-NLP-cMyc-cMyc AspCas12a

    Plasmid

    #182122

    Purpose

    pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    3xNLS-NLP-cMyc-cMyc AspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Px458-Cas9n-Trp63-Exon4-2sgRNA

    Plasmid

    #88849

    Purpose

    CRISPR KO of Trp63

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp63 (Trp63 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

13. pMS28: pTns(PmcCAST)_ΔTniQ,ΔTnsD

    Plasmid

    #168161

    Purpose

    Inducible expression of PmcCAST TnsAB and TnsC proteins.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    PmcCAST Tns proteins (TnsAB and TnsC)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLS

    Plasmid

    #121821

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-dCas9 (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUPD2 nickase D10A Cas9 (GB1690)

    Plasmid

    #160587

    Purpose

    Nickase D10A Cas9 for Nt and Ct fusion.

    Depositor

    Diego Orzaez

    Insert

    nickase D10A Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6

    Plasmid

    #159913

    Purpose

    Mutagenesis of Kcnj6

    Depositor

    Larry Zweifel

    Insert

    Kcnj6 (Kcnj6 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCSDest2-SpCas9-NLS-3XHA-NLS

    Plasmid

    #69220

    Purpose

    Expresses wild type SpCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti SYN-SVI-dCas9-VPR

    Plasmid

    #164575

    Purpose

    Expresses an intron-containing dCas9-VPR fusion driven by human SYN promoter

    Depositor

    Jeremy Day

    Article

    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

    Insert

    FLAG-dCas9-VPR containing an SV40 intron
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-Flag-wtSpCas9 (without sgRNA)

    Plasmid

    #92353

    Purpose

    Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFlag-NLS-wild-type SpCas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106353

    Purpose

    CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    D4Z4 gRNA-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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