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pTopo RE3 Donor pLox hPGK-Puro pA pLox

Plasmid

#171050

Purpose

Donor template for CRISPR/Cas9 targeting of TERT RE3

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

Puromycin

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 K478R/479R D421N

Plasmid

#167200

Purpose

puromycin resistant lentiviral vector for expression of PAK5 K478R/479R D421N; PAK7 Kinase dead & D421N double mutant

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 K478R/479R D421N

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 K478R/479R E294K

Plasmid

#167198

Purpose

puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E294K; PAK7 Kinase dead & E294K double mutant

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 K478R/479R E294K

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 K478R/479R M173I

Plasmid

#167197

Purpose

puromycin resistant lentiviral vector for expression of PAK5 K478R/479R M173I; PAK7 Kinase dead & M173I double mutant

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 K478R/479R M173I

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 K478R/479R E144K

Plasmid

#167196

Purpose

puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E144K; PAK7 Kinase dead & E144K double mutant

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 K478R/479R E144K

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(L301R/V303R)-IRES-puro

Plasmid

#128658

Purpose

mammalian expression of Flag-Polß(L301R/V303R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)

Plasmid

#149350

Purpose

Mammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNA

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

SpyCas9

Use

CRISPR

Tags

Flag tag

Expression

Mammalian

Promoter

Cbh

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(L301R/V306R)-IRES-puro

Plasmid

#128656

Purpose

mammalian expression of Flag-Polß(L301R/V306R) with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V306R

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(K206A/K244A)-IRES-puro

Plasmid

#128683

Purpose

mammalian expression of Flag-Polß(K206A/K244A) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K206A/K244A

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(TM/K206A/K244A)-IRES-puro

Plasmid

#128684

Purpose

mammalian expression of Flag-Polß(TM/K206A/K244A: L301R/V303R/V306R/K206A/K244A)) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag tag

Expression

Mammalian

Mutation

L301R/V303R/V303R/K206A/K244A

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Thy-1-CRISPR-gRNA#1-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124283

Purpose

sgRNA against human Thy-1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)

Use

CRISPR

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
Thy-1-CRISPR-gRNA#3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124285

Purpose

sgRNA against human Thy-1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)

Use

CRISPR

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
Thy-1-CRISPR-gRNA#2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124284

Purpose

sgRNA against human Thy-1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)

Use

CRISPR

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh1

Plasmid

#123324

Purpose

Lentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

shRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pZIP-hCMV-ZsGreen-P2A-Puro-IKZF3 sh1

Plasmid

#123326

Purpose

Lentiviral vector for expression of shRNA targeting IKZF3 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

shRNA targeting IKZF3 embedded in miR30 backbone (IKZF3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh2

Plasmid

#123325

Purpose

Lentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

shRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pZIP-hCMV-ZSGreen-P2A-Puro-IKZF3 sh2

Plasmid

#123327

Purpose

Lentiviral vector for expression of shRNA targeting IKZF3 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

shRNA targeting IKZF3 embedded in miR30 backbone (IKZF3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCDF1-MCS2-EF1-Puro-RGS7-R44C+V56C

Plasmid

#113730

Purpose

Express double mutant RGS7- R44C+V65C in mamalian cells

Depositor

Yardena Samuels

Article

Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

Insert

RGS7 (RGS7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R44C and V56C

Promoter

CMV

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only
HA-HIF2α P405A/N847A Δ450-572-pBabe-Puro

Plasmid

#25936

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

Plasmid

#71236

Purpose

Express sgRNA and dCas9-KRAB from lentiviral vector

Depositor

Charles Gersbach

Article

Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

Inserts

humanized dCas9-KRAB T2A Puro
sgRNA

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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