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We narrowed to 1,401 results for: pp

Showing: 901 - 950 of 1401 results

  9. pGL4.23 A82V/T230A/D637G 2014 EBOV Delta-Mucin-Like-Domain

    Plasmid
    #86026
    Purpose
    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector
    Depositor
    Pardis Sabeti, Jeremy Luban
    Article
    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do
    Insert
    Makona Ebola Virus Glycoprotein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Lacks mucin-like domain; Changed alanine 82 to va…
    Promoter
    CMV IE
    Available since
    July 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    86026-plasmid-map-sequence-id-169924

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  11. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90)

    Plasmid
    #226989
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1a must be used with gRNA 2a or 2b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 1a to create OPA1-del_exon5
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226989-plasmid-map-sequence-id-453964

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  12. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91)

    Plasmid
    #226991
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 2a to create OPA1-del_exon5
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226991-plasmid-map-sequence-id-453954

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  15. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

    Plasmid
    #226992
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 2b to create OPA1-del_exon5
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    226992-plasmid-map-sequence-id-451253

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  16. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)

    Plasmid
    #226990
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 1b to create OPA1-del_exon5
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    226990-plasmid-map-sequence-id-451250

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  18. pFB1.HMBP.PrS.CBX8ΔChromo

    Plasmid
    #224708
    Purpose
    Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag
    Depositor
    Chen Davidovich
    Article
    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.
    Insert
    CBX8 chromodomain truncation (CBX8 Human)
    Use
    Tags
    HMBP
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    Oct. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    224708-plasmid-map-sequence-id-449678

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  37. 4MYJ

    Plasmid
    #60104
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PF14_0346:MAC04G-A11:C228843
    Use
    Baculovirus expression
    Tags
    6xHis-TEV and MHHHHHHSSGRENLYFQG
    Expression
    Mutation
    See comments
    Promoter
    Polyhedrin
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. 4QNS

    Plasmid
    #61957
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PF08_0034:MAC04J-B11:C229648
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7-lacO
    Available since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. 4MYI

    Plasmid
    #60103
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PVX_084705:MAC04G-C11:C228845
    Use
    Baculovirus expression
    Tags
    6x His and TEV cleavage site
    Expression
    Mutation
    See comments
    Promoter
    polyhedrin
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  41. pSCAR_Cas9-blast_GFP

    Plasmid
    #162074
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-blasticidin S deaminase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162074-plasmid-map-sequence-id-471574

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  49. pSCAR_Cas9-hygro_GFP

    Plasmid
    #162075
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-hygromycin B phosphotransferase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162075-plasmid-map-sequence-id-325304

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Showing: 901 - 950 of 1401 results