We narrowed to 13,831 results for: Cas9

1. dCAS9-VP64_GFP

   Plasmid

   #61422

   Purpose

   Expresses dCAS9-VP64 activator with 2A GFP

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Concentrated Lentiviral Prep

   Insert

   dCAS9(D10A,H840A)-VP64_2A_GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and H840A in Cas9

   Promoter

   EF1A

   Available since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpCas9-CBE6b

   Plasmid

   #215820

   Purpose

   Express CBE6b (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6b-SpCas9-2xUGI (cas9 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AB65_pCAG.Cas9-D10A.rBGpA

   Plasmid

   #199256

   Purpose

   Expression construct encoding the SpCas9-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   SpCas9.D10A nickase
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPR v2-dCas9

   Plasmid

   #112233

   Purpose

   Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2

   Depositor

   Thomas Gilmore

   Article

   Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do

   Inserts

   dCas9
   Porumycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed: Aspartic acid 10 to Glycine, Histidine 8…

   Promoter

   EFS-NS

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pdCas9-bacteria

   Plasmid

   #44249

   Purpose

   aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dCas9 (bacteria)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   pLtetO-1

   Available since

   April 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cas9m4-VP64

   Plasmid

   #47319

   Purpose

   Cas9m4 Activator

   Depositor

   George Church

   Article

   Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

   Insert

   Cas9m4-VP64
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEND-351_Cacnes_dCas9_CRISPRi

   Plasmid

   #225617

   Purpose

   Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Promoter

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas9-CBP FL

   Plasmid

   #179550

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. B-Sniper SpCas9

   Plasmid

   #207361

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpdCas9 VPR

    Plasmid

    #188509

    Purpose

    Expresses FLAG tagged SpdCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9_T2A_iRFP670 sgBFP

    Plasmid

    #192478

    Purpose

    All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9(BB)-2A-Blast

    Plasmid

    #118055

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9-HF1

    Plasmid

    #138556

    Purpose

    Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    SpCas9-HF1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-eSpCas9_sgAAVS1

    Plasmid

    #199213

    Purpose

    All-in-one plasmid encoding eSpCas9 and sgRNA targeting the AAVS1 site in human cells. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    AAVS1-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    human U6

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. 2X_pX458_pSpCas9(BB)-2A-GFP

    Plasmid

    #172221

    Purpose

    Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. VPR-ps-dSpCas9

    Plasmid

    #102855

    Purpose

    A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    dSpCas9, pdDronpa1.2
    

    Use

    CRISPR and Synthetic Biology

    Tags

    NLS and VP64-p64-Rta transcription activator

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-21a_3xNLS_SpCas9_protein_expression

    Plasmid

    #114365

    Purpose

    Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

    Insert

    3xNLS_SpCas9
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. dCas9 plasmid

    Plasmid

    #100091

    Purpose

    dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFlag-NLS-dCas9-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. eSpCas9(1.1)

    Plasmid

    #71814

    Purpose

    Expresses high specificity SpCas9. Px330-like plasmid.

    Depositor

    Feng Zhang

    Article

    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

    Has Service

    Cloning Grade DNA

    Insert

    enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDicAID_nCas9-PmCDA_NptII_Della

    Plasmid

    #91694

    Purpose

    Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A for nickase Cas9

    Promoter

    PcUbi

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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