We narrowed to 4,345 results for: p2a

1. pH6HTC_IGF2BP2a-Halo

   Plasmid

   #175309

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   IGF2BP2 (IGF2BP2 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO5d.EFS.SpCas9.P2A.PAC

   Plasmid

   #58329

   Purpose

   Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   EFS (EEF1A1 Human)
   P2A-PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR

   Plasmid

   #81251

   Purpose

   Lentiviral construct for building stable cell lines expressing Cas9 via puromycin selection

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   Cas9-3xNLS
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV_iRFP_P2AT2A_mScarlet-I_Myl9

   Plasmid

   #172474

   Purpose

   Mammalian expression of myosin light chain (Myl9) tagged with mScarlet-I and cytoplasmic iRFP as a reference marker.

   Depositor

   Sean R Collins

   Article

   Hadjitheodorou et al Nat Commun. 2021 Nov 16;12(1):6619. doi:

   Inserts

   iRFP670
   mScarlet-I-Myl9 (MYL9 Human)
   

   Use

   Lentiviral

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha (with UCOE) and none (same transcript as…

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVP2A-PIF6

   Plasmid

   #73369

   Purpose

   Expresses Adeno-associated virus VP2 with PIF6 insert.

   Depositor

   Junghae Suh

   Article

   Gomez et al ACS Nano. 2015 Nov 30.

   Insert

   PIF6 (PIL2 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. TOP2A sgRNA1

   Plasmid

   #138190

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TOP2A

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TOP2A sgRNA2

   Plasmid

   #138191

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TOP2A

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP

   Plasmid

   #162233

   Purpose

   Lentiviral vector for the expression of GFP nanobody LaG16LaG2 -IFP : TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   anti-GFP nanobody lag16lag2 and IFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCellFree_G03 CYP2A13

   Plasmid

   #67103

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CYP2A13 (CYP2A13 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. EF1a_MYC Thr58Ala_P2A_Hygro_Barcode

    Plasmid

    #120513

    Purpose

    Barcoded lentiviral vector to express MYC Thr58Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC Thr58Ala (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Point mutation changing Threonine to Alanine at a…

    Promoter

    EF1a

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. EF1a_MYC deltaNLS_P2A_Hygro_Barcode

    Plasmid

    #120506

    Purpose

    Barcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC H84NLS (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of nuclear localization signal sequence

    Promoter

    EF1a

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIneoLuc-PP2A

    Plasmid

    #128349

    Purpose

    Vector for LUMIER assay

    Depositor

    Yutaka Hata

    Article

    Kawano et al J Biochem. 2015 May 14. pii: mvv051.

    Insert

    PP2A (PTPA Human)
    

    Use

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. CIP2Aprom1802bp-pGL4.10Luc

    Plasmid

    #60868

    Purpose

    Luc reporter driven by 1802 bp of the CIP2A promoter

    Depositor

    Jukka Westermarck

    Article

    Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

    Insert

    1802 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)
    

    Use

    Luciferase; Promoterless

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR223-LMP2A

    Plasmid

    #195816

    Purpose

    Gateway Entry Clone of Epstein-Barr virus LMP2A

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    LMP2A (LMP-2A Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a_MYC Ser62Ala_P2A_Hygro_Barcode

    Plasmid

    #120514

    Purpose

    Barcoded lentiviral vector to express MYC Ser62Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC Ser62Ala (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Point mutation changing Serine to Alanine at amin…

    Promoter

    EF1a

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

    Plasmid

    #107736

    Purpose

    In the presence of Cre, can be used to express both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.

    Depositor

    Rylan Larsen

    Insert

    synaptophysin-GCaMP6s, mRuby3
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCHA1.1-Rap2a-V5

    Plasmid

    #149509

    Purpose

    Stable expression of human Rap2a.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    RAP2 (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Codon Optimized Sequence

    Promoter

    Ubc

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDTnLAPP2A frame 0

    Plasmid

    #28226

    Depositor

    Harald von Melchner

    Article

    Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

    Insert

    Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein
    

    Use

    Retroviral

    Tags

    EGFP and nLAP-tag

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pP2AeGFP-Mars1Cy

    Plasmid

    #75223

    Purpose

    Mammalian intracellular expression of fluorogen activating protein Mars1Cy and eGFP

    Depositor

    Alan Waggoner

    Article

    Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

    Insert

    EGFP-p2a-Mars1Cy
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCellFree_G03 RAP2A

    Plasmid

    #67058

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only