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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 12,558 results for: 109

Showing: 3801 - 3820 of 12558 results

  1. pET28-NFHΔ36-72

    Plasmid
    #232060
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Heavy tail domain lacking residues 36-72
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    added N-terminal tryptophan and tyrosine, and cys…
    Promoter
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232060-plasmid-map-sequence-id-464069

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  2. pET28-NFHΔ462-638

    Plasmid
    #232061
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Heavy tail domain lacking residues 462-638
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    added N-terminal tryptophan and tyrosine, and cys…
    Promoter
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232061-plasmid-map-sequence-id-464068

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  3. pET28-NFLtail

    Plasmid
    #232055
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFL tail domain
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Light tail domain
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    cDNA codon-optimized for E. coli; added N-termina…
    Promoter
    T7
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232055-plasmid-map-sequence-id-464065

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  4. pET28-NFMtail

    Plasmid
    #232056
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFM tail domain
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Medium tail domain
    Use
    Tags
    6xHis, thrombin
    Expression
    Bacterial
    Mutation
    cDNA codon-optimized for E. coli; added N-termina…
    Promoter
    T7
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232056-plasmid-map-sequence-id-464064

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  5. pET28-NFMshuf1

    Plasmid
    #232058
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 1
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Medium tail domain, charge shuffle 1
    Use
    Tags
    6xHis, thrombin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232058-plasmid-map-sequence-id-464062

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  6. pET28-NFHtail

    Plasmid
    #232057
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFH tail domain
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Heavy tail domain
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    added N-terminal tryptophan and tyrosine, and cys…
    Promoter
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232057-plasmid-map-sequence-id-464061

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  7. pET28-NFMshuf2

    Plasmid
    #232059
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Medium tail domain, charge shuffle 2
    Use
    Tags
    6xHis, thrombin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232059-plasmid-map-sequence-id-464059

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  8. SARS-CoV-2 spike G12345 mutant

    Plasmid
    #231920
    Purpose
    For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1134, N1158, N1173, N1194 by implementing Asn-to-Gln mutation
    Depositor
    Sriram Neelamegham
    Article
    Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10
    Insert
    spike G12345 (S SARS-CoV-2)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    N1098Q, N1134Q, N1158Q, N1173Q, N1194Q
    Promoter
    CMV
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231920-plasmid-map-sequence-id-458823

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  15. pCS2+-Zeo-miR-430-G-G mm

    Plasmid
    #221728
    Purpose
    Expression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.
    Depositor
    David Bartel
    Article
    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5
    Insert
    BleoR-miR-430-G-G mm
    Use
    Fish expression
    Tags
    Expression
    Mutation
    G-G mismatch of miR-430 site
    Promoter
    simian CMV IE94
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221728-plasmid-map-sequence-id-449658

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  16. pCS2+-Zeo-miR-430-10-11 mm

    Plasmid
    #221727
    Purpose
    Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.
    Depositor
    David Bartel
    Article
    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5
    Insert
    BleoR-miR-430-10-11 mm
    Use
    Fish expression
    Tags
    Expression
    Mutation
    Mismatch at positions 10 and 11 of miR-430 site
    Promoter
    simian CMV IE94
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221727-plasmid-map-sequence-id-449656

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Showing: 3801 - 3820 of 12558 results