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pGEX Tyk2-SH2

Plasmid

#46527

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Tyk2 (TYK2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 452-567

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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TTMP-His-bio

Plasmid

#52335

Purpose

Expresses full-length TPA-induced transmembrane protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

TTMP (C3orf52 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot9_18-285_S

Plasmid

#147480

Purpose

Mammalian Expression of HsNot9_18-285

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9_18-285 (CNOT9 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot9_18-285_S

Plasmid

#147482

Purpose

Mammalian Expression of HsNot9_18-285

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9_18-285 (CNOT9 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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CD8a-hnRNP-M-EGFP

Plasmid

#86054

Purpose

A mammalian expression plasmid encoding CD8a luminal and transmembrane domains followed by hnRNP-M (PY nuclear localization signal of hnRNP-M) and C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

CD8a (CD8A Human)

Tags

GFP

Expression

Mammalian

Mutation

CD8a luminal and transmembrane domians along with…

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNotch2#2/Cre

Plasmid

#193226

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch2 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNotch2#2 (Notch2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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mTFP1-MAPTau-N-10

Plasmid

#55498

Purpose

Localization: Microtubules, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

MAPTau (MAPT Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
MSCV-TAP(FLAG-HA) CYLD

Plasmid

#60031

Purpose

CYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintained

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Use

Retroviral

Tags

FLAG, HA, and IRES Puro

Expression

Mammalian

Promoter

LTR-driven expression

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pHH0103_CRK-2/2-SV1/2

Plasmid

#91319

Purpose

Protein expression and purification of human SH3 domain construct CRK-2/2-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CRK-2/2-SV1/2 (CRK Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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DMGDH

Plasmid

#155549

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DMGDH (DMGDH Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pT5T_hp53_ST_CR2

Plasmid

#34923

Depositor

Thanos Halazonetis

Article

Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

Insert

p53 DNA-binding (Res 94-358, deletion 293-321) (TP53 Human)

Expression

Bacterial

Mutation

C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

Promoter

T7

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-TRR-C421-Y2404F

Plasmid

#182846

Purpose

mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

Depositor

Peter Harte

Article

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

TRR 2011-2431 (trr Fly)

Expression

Bacterial

Mutation

mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2K930R-FF-VSV

Plasmid

#139348

Purpose

Expression of kinase-dead TYK2-K930R missing the two tyrosines at the activation loop

Depositor

Sandra Pellegrini

Article

Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-K930R-Y1054F-Y1055F

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK21054-5FF-VSV

Plasmid

#139347

Purpose

Expression of TYK2 protein mutated in the two tyrosines (Y1054F/Y1055F) of the activation loop

Depositor

Sandra Pellegrini

Article

Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Expression

Mammalian

Mutation

V362F-Y1054F-Y1055F

Promoter

CMV

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-TACC3KDP(S558A)-shTACC3

Plasmid

#59357

Purpose

Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Use

RNAi

Tags

GFP

Expression

Mammalian

Mutation

Silent mutations in amino acids 30-33 to confer s…

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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CSK_HUMAN_D0

Plasmid

#79683

Purpose

This plasmid encodes the kinase domain of CSK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CSK (CSK Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
ApoE2_mCh-SspB (pBS1142)

Plasmid

#185324

Purpose

For the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE2

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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MI-Luciferase

Plasmid

#75018

Purpose

Encodes the expression of Luciferase (with out any fluorescent reporter)

Depositor

Xiaoping Sun

Article

Mallampati et al Blood. 2015 May 7;125(19):2968-73. doi:

Insert

firefly Luciferase

Use

Luciferase and Retroviral

Available Since

May 31, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-4

Plasmid

#112844

Purpose

transient/stable overexpression of the N-terminal and central domains of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

N + C1 domains of Catulin

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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WFS1 mRFP-N2 (891)

Plasmid

#62018

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

WFS1 (WFS1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only