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pET21-E. coli Initiation Factor 3 (IF3)

Plasmid

#31340

Depositor

Linda Spremulli

Article

Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

Insert

E. coli Initiation Factor 3

Tags

His

Expression

Bacterial

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pFastBac1 hBRM

Plasmid

#1956

Depositor

Robert Kingston

Article

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

BRM (SMARCA2 Human)

Tags

flag

Expression

Insect

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
PEG-Actinin-M-sstFRET

Plasmid

#61100

Purpose

Expression of α-actinin with internal sstFRET inserted to measure cytoskeletal stress in vivo

Depositor

Fred Sachs

Article

Meng et al J Cell Sci. 2011 Jan 15;124(Pt 2):261-9.

Insert

α-actinin-sstFRET

Expression

Mammalian

Mutation

sstFRET inserted at position 300

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV5B ActR-IB

Plasmid

#14050

Depositor

Joan Massague

Article

Attisano et al Mol Cell Biol. 1996 Mar . 16(3):1066-73.

Insert

ActR-IB (ACVR1B Human)

Expression

Mammalian

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3 Rtf1

Plasmid

#11060

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

Rtf1 (RTF1 Human)

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
CMV-HA-hTAF5

Plasmid

#17984

Depositor

Naoko Tanese

Article

Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

Insert

TAF5 (TAF5 Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBY2982

Plasmid

#23220

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

BirA-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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p1990 SV40-LMP1

Plasmid

#26654

Depositor

Bill Sugden

Article

Kaykas et al EMBO J. 2001 Jun 1. 20(11):2641-54.

Insert

Latent Membrane Protein 1 (LMP-1 Epstein-Barr Virus)

Expression

Mammalian

Available Since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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pBEVY-GT

Plasmid

#51231

Purpose

bi-directional expression vector for yeast, galactose inducible, TRP1 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GAL1,10

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pNB2176

Plasmid

#113395

Purpose

Ty3-IN-GFP; alpha factor inducible

Depositor

Suzanne Sandmeyer

Article

Beliakova-Bethell et al RNA. 2006 Jan;12(1):94-101. doi: 10.1261

Insert

Ty3 with integrase-green fluorescent protein

Tags

IN-GFP

Expression

Yeast

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBS_36x-601_MA1+MA3+MA2

Plasmid

#114364

Purpose

36x Widom 601 nucleosome sequence

Depositor

Scot Wolfe

Article

Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

Insert

36X601-MA1_3_2

Use

Unspecified

Expression

Mammalian

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS

Plasmid

#19446

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HIS

Expression

Mammalian

Mutation

HIS tag inserted to create new vector

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pESC-URA-GFP-Ubc9wt

Plasmid

#20368

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pHLU

Plasmid

#64167

Purpose

harbours marker genes HIS3, LEU2 and URA3

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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Monomer NH_V2

Plasmid

#37527

Purpose

template for PCR amplification of the monomer used for assembly of the DNA-binding tandem repeat region of the TALE.

Depositor

Feng Zhang

Article

Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

Insert

Monomer NH

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
438-GST

Plasmid

#97092

Purpose

GST-tev-yORF

Depositor

Chris Jeans

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

GST tag and TEV cleavage site

Expression

Insect

Promoter

Polyhedrin promoter

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMBP-HIS2-Vps2

Plasmid

#21494

Depositor

James Hurley

Article

Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

Insert

VPS2 (DID4 Budding Yeast)

Tags

6x His and MBP

Expression

Bacterial

Mutation

none

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
PlayBack-U6-Xba1

Plasmid

#203300

Purpose

For expression of sgRNAs in a PlayBack Vector with XbaI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA47_Helper

Plasmid

#231420

Purpose

M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ296.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

gp1-gp11

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 VPS50

Plasmid

#175500

Purpose

VPS50-GFP is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Schindler et al Nat Cell Biol. 2015 May;17(5):639-50. do

Insert

Vps50 (Vps50 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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