We narrowed to 28,357 results for: CAL

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pUASTLOTattB_vhhGFP4::T48-Baz::mCherry

Plasmid

#163927

Purpose

Expression of GrabFP-A-extracellular under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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iSKetSnFR2

Plasmid

#140467

Purpose

Develop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR2, thus enabling optical subcellular pharmacokinetics for S-Ketamine.

Depositor

Henry Lester

Article

Bera et al Front Cell Neurosci. 2019 Nov 12;13:499.

Insert

iSKetSnFR2

Tags

His Tag, HA Tag and Myc tag

Expression

Bacterial

Promoter

T7

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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iSKetSnFR1

Plasmid

#140466

Purpose

Develop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR1, thus enabling optical subcellular pharmacokinetics for S-Ketamine.

Depositor

Henry Lester

Article

Bera et al Front Cell Neurosci. 2019 Nov 12;13:499.

Insert

iSKetSnFR1

Tags

His Tag, HA Tag and Myc tag

Expression

Bacterial

Promoter

T7

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3

Plasmid

#111922

Purpose

Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μ

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_1.2μ

Tags

CoxVIIIx2

Expression

Mammalian

Mutation

E67D, E104D, D133E at CaM

Promoter

CMV

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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Twitch-2C pRSETB

Plasmid

#104500

Purpose

fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2C

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only
AY23_pgRNA.R5.2

Plasmid

#100295

Purpose

CCR5-targeting gRNA expression plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

gRNA spacer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6 Polymerase III snRNA

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO CD16.7-h-PKD1 FLM W4108A

Plasmid

#83462

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (502)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4303, W4107A

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO CD16.7-h-PKD1 FLM S4144D

Plasmid

#83460

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a point mutant that disrupts the second calmodulin binding domain (513)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4303, S4144D

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS S4144D-Myc/His

Plasmid

#83459

Purpose

Human PC1-CTp30 expression cassette with a point mutant that disrupts the second calmodulin binding domain (514)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Amino-acids 4107-4303, S4144D

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His

Plasmid

#83457

Purpose

Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) (632)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Amino-acids 4127-4303

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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tdminiSOG-Tubulin-6

Plasmid

#57808

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Tubulin (TUBA1B Human)

Tags

tdminiSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
psicheck-2-Mc2r

Plasmid

#48174

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of MC2R (MC2R Human)

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
pEXP5-NT-5th R

Plasmid

#42572

Depositor

Ivan Korendovych

Article

Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

Insert

mutated calmodulin gene

Tags

Polyhistidine (6×His) region, TEV recognition sit…

Expression

Bacterial

Mutation

changed Phenylalanine 92 to Glutamine acid, Isole…

Promoter

T7

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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ATMY-C321

Bacterial Strain

#218774

Purpose

E. coli strain, genotype: C321.ΔA pUltraBR-MjY ΔtyrS tyrTV::tolC tyrU::GentR lambda::ZeoR

Depositor

Abhishek Chatterjee

Article

Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

Bacterial Resistance

Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL)

Species

Escherichia coli

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only
LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain

Bacterial Strain

#99247

Purpose

LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain

Depositor

Jeff Hasty

Article

Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

Bacterial Resistance

Tetracycline

Available Since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP5(nth-ydgR)

Bacterial Strain

#110245

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
C321.ΔClpS.Ub-UAG-sfGFP

Bacterial Strain

#98565

Purpose

Recoded strain C321.ΔA (MutS inactivated) with genomically integrated Ubiquitin-UAG-sfGFP reporter and ClpS inactivated

Depositor

George Church

Article

Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

Bacterial Resistance

Ampicillin

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_fLP4(essQ-cspB)

Bacterial Strain

#110244

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the essQ-cspB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP6(ygcE-ygcF)

Bacterial Strain

#110246

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the ygcE-ygcF intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP1(atpI-gidB)

Bacterial Strain

#110241

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the atpl-gidB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only