We narrowed to 51,260 results for: des.1
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Plasmid#197421PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorInsertMAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
UseCRISPRAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNXF-1-R233AR276A_O
Plasmid#147120PurposeMammalian Expression of HsNxf-1-R233AR276ADepositorInsertHsNxf-1-R233AR276A (NXF1 Human, See Depositor Comments Below)
ExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
RPS15A sgRNA-1 lentiCRISPR v2 plasmid
Plasmid#192227Purposelentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS15A 5'UTRDepositorInsertsgRNA-1 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
UseCRISPR and LentiviralAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-PD-1 (BTLA ITIM)-mGFP
Plasmid#180817PurposeExpression of human PD-1 with immunoreceptor tyrosine-based inhibitory motif (ITIM) from BTLA, fused to mEGFPDepositorInsertPD-1 (BTLA ITIM) (PDCD1 Human)
UseLentiviralTagsmEGFPMutationreplaced PD-1-ITIM (VDYGEL) with BTLA-ITIM (IVYAS…PromoterSFFVAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3/flag-pBirA(1-K283)-FRB
Plasmid#154451Purposeexpresses flag-pBirA(1-K283)-FRB/biochemical assayDepositorInsertflag-pBirA(1-K283)-FRB
ExpressionMammalianPromoterT7 promoterAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN(GFP)-HA-Tbkbp1 1-280
Plasmid#125178PurposeRetroviral expression of mouse Tbkbp1 (1-280)DepositorInsertTbkbp1 (Tbkbp1 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationContains amino acids 1-280PromoterCMVAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN(GFP)-HA-Tbkbp1 1-330
Plasmid#125179PurposeRetroviral expression of mouse Tbkbp1 (1-330)DepositorInsertTbkbp1 (Tbkbp1 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationContains amino acids 1-330PromoterCMVAvailable SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shGDF11 #2
Plasmid#83084PurposeLentiviral shRNA vector for inducible knockdown of human GDF11DepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-218-1-3p
Plasmid#103356PurposeUsed to sense miRNA activity using fluorescence based tools. hsa_miR-218-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa_miR-218-1-3p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLPCX2-HA-GATA3 N1(1-259)
Plasmid#89830PurposeExpress GATA3 N1 in mammalian cellsDepositorAvailable SinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLPCX2-HA-GATA3 N2(1-294)
Plasmid#89831PurposeExpress GATA3 N2 in mammalian cellsDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)
Plasmid#61941PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coliDepositorTags6x HISExpressionBacterialMutationConstruct ends at residue 117, stop codon introdu…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-645, Δ368-492)/pcDNA3.1
Plasmid#111454PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
ExpressionMammalianMutationKv7.4 (1-645, Δ368-492), C643AAvailabilityAcademic Institutions and Nonprofits only -
PGC-1 alpha promoter 2kb luciferase
Plasmid#8887DepositorAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-mRFP-; gcry1:BFP -1
Plasmid#173887PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; gcry1: BFP
UseSynthetic BiologyAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
CHOP promoter (-649/+136) pmCherry-1
Plasmid#36035DepositorInsertCHOP promoter (-647 to +136) (DDIT3 Human)
ExpressionMammalianAvailable SinceAug. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-1 (A317-318; A341)
Plasmid#204194PurposeMammalian expression of SREBP-1 (Isoform 1)DepositorInsertSREBP-1 (SREBF1 Human)
TagsE-tag and T7x2ExpressionMammalianMutationGly to Ala 317,318,341Available SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRM919_WT aa 1-419 His POLI
Plasmid#201445PurposeLow copy vector for expressing the catalytic domain (amino acids 1-419) of human DNA polymerase iota in E. coli with an N-terminal 6x His tag. C-terminal truncation mutant of pJRM919_WT His POLIDepositorInsertDNA polymerase iota (POLI Human)
Tags6x HisExpressionBacterialMutationExpresses amino acids 1-449 of DNA polymerase iot…Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7f-1-3p
Plasmid#103154PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7f-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7f-1-3p target (MIRLET7F1 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pN1-RnKIF5C(1-560)-Halo-Flag
Plasmid#188071PurposeMammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tagsDepositorAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only