We narrowed to 51,047 results for: Ide

1. pLenti-X1-Neo-DDRGK1-K116R-HA

   Plasmid

   #139850

   Purpose

   Lentiviral expression of human DDRGK1-K116R-HA

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   DDRGK1 (DDRGK1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   K116R

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-X1-Neo-DDRGK1-K120R-HA

   Plasmid

   #139851

   Purpose

   Lentiviral expression of human DDRGK1-K120R-HA

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   DDRGK1 (DDRGK1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   K120R

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-X1-Neo-DDRGK1-K124R-HA

   Plasmid

   #139853

   Purpose

   Lentiviral expression of human DDRGK1-K124R-HA

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   DDRGK1 (DDRGK1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   K124R

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-X1-Neo-DDRGK1-K128R-HA

   Plasmid

   #139854

   Purpose

   Lentiviral expression of human DDRGK1-K128R-HA

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   DDRGK1 (DDRGK1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   K128R

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-X1-Neo-DDRGK1-K146R-HA

   Plasmid

   #139855

   Purpose

   Lentiviral expression of human DDRGK1-K146R-HA

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   DDRGK1 (DDRGK1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   K146R

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-X1-Neo-DDRGK1-K176R-HA

   Plasmid

   #139856

   Purpose

   Lentiviral expression of human DDRGK1-K176R-HA

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   DDRGK1 (DDRGK1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   K176R

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-X1-Neo-DDRGK1-K193R-HA

   Plasmid

   #139857

   Purpose

   Lentiviral expression of the cDNA indicated

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   DDRGK1 (DDRGK1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   K193R

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-X1-Neo-DDRGK1-K224R-HA

   Plasmid

   #139858

   Purpose

   Lentiviral expression of human DDRGK1-K224R-HA

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   DDRGK1 (DDRGK1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   K224R

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a_6HT-TBP6.7(R47A)

   Plasmid

   #112722

   Purpose

   Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a_6HT-TBP6.7(R47K)

    Plasmid

    #112723

    Purpose

    Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47K)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47K

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a_6HT-TBP6.7(Q48A)

    Plasmid

    #112724

    Purpose

    Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a_6HT-TBP6.7(T50A)

    Plasmid

    #112727

    Purpose

    Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(T50A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    T50A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a_6HT-TBP6.7(R52A)

    Plasmid

    #112729

    Purpose

    52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R52A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R52A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a_6HT-TBP6.7(delta C-term)

    Plasmid

    #112731

    Purpose

    This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(delta C-term)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    delta C-term

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. HeFm1SpCas9

    Plasmid

    #92110

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFRT-TODestGFP_RBPMS2

    Plasmid

    #65747

    Purpose

    expresses GFP-tagged RBPMS2 in mammalian cells

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS2 (RBPMS2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S13N mutation in RBPMS2

    Promoter

    CMV, 2x Tet

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPICZ A-Strep-Opa1 (SB251)

    Plasmid

    #227600

    Purpose

    Inducible expression of Strep-tagged Opa1 in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    OPA1 (OPA1 Human)
    

    Tags

    Twin-StrepTag

    Expression

    Yeast

    Mutation

    contains aa 253-960 only

    Promoter

    AOX1

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-hMC4R

    Plasmid

    #179315

    Purpose

    Express human melanocortin 4 receptor (GFP tagged) in mammalian cells

    Depositor

    Christian Gruber

    Article

    Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

    Insert

    melanocortin 4 receptor (MC4R Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-mKOR

    Plasmid

    #179285

    Purpose

    Express mouse kappa opioid receptor (GFP tagged) in mammalian cells

    Depositor

    Christian Gruber

    Article

    Muratspahic et al J Med Chem. 2021 Jul 8;64(13):9042-9055.

    Insert

    kappa opioid receptor (Oprk1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti6.3-CD300C-V5

    Plasmid

    #124089

    Purpose

    CD300C-V5 tagged expression vector

    Depositor

    J. Chad Brenner

    Article

    Mann et al Oral Oncol. 2023 Nov;146:106562. doi: 10

    Insert

    CD300C (CD300C Human)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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