We narrowed to 11,256 results for: NSI
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Plasmid#35611Purposemammalian expression of mouse Alpha6 (Chrna6) with internal mEGFP tagDepositorInsertnAChr alpha6mEGFP (Chrna6 Mouse)
UseTagsmEGFP inserted after A405 with AGA linker on eith…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLL6 sgRNA(MS2)-MS2-hA3a D131E
Plasmid#112132Purposeempty sgRNA cloning vector with MS2-humanA3a D131EDepositorInserthuman Apobec3a D131E mutant (APOBEC3A Human)
UseCRISPRTagsExpressionMammalianMutationD131E mutant in hA3aPromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_DM1-3_WT-ABD
Plasmid#234553PurposeCTNNA1 M-domain deletion mutantDepositorInsertmEGFP:hCTNNA1_DM-domain (CTNNA1 Human)
UseLentiviralTagsmEGFPExpressionMutationDeletion of aa 273-635PromoterCMVAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_L436P
Plasmid#234559PurposeCTNNA1 eye phenotype via forward genetic screenDepositorInsertmEGFP:hCTNNA1_L436P (CTNNA1 Human)
UseLentiviralTagsmEGFPExpressionMutationL436PPromoterCMVAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_DNii domain
Plasmid#234557PurposeCTNNA1 Nii-domain deletion mutantDepositorInsertmEGFP:hCTNNA1_DNii domain (CTNNA1 Human)
UseLentiviralTagsmEGFPExpressionMutationDeletion of aa ~165-265PromoterCMVAvailable sinceJune 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA377 - pBA904 Puro-T2A-GFP CD55 CRISPRa guide 1 (pRCA360 backbone)
Plasmid#238168PurposeLentiviral CRISPR guide vector expressing CD55 targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorInsertCD55 sgRNA CRISPRa (CD55 Synthetic, Human)
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA803 - pBA904 Puro-T2A-GFP KLF5 g1 CRISPRa guide (pRCA360 backbone) 666
Plasmid#238172PurposeLentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorInsertKLF5 sgRNA CRISPRa (KLF5 Synthetic, Human)
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA804 - pBA904 Puro-T2A-GFP KLF5 g2 CRISPRa guide (pRCA360 backbone) 665
Plasmid#238173PurposeLentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorInsertKLF5 sgRNA CRISPRa (KLF5 Synthetic, Human)
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA810 - pBA904 Puro-T2A-GFP HES7 g2 CRISPRa guide (pRCA360 backbone) 674
Plasmid#238177PurposeLentiviral CRISPR guide vector expressing a HES7 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorInsertHES7 sgRNA CRISPRa (HES7 Synthetic, Human)
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA895 - pBA904 Puro-T2A-BFP KLF4 g1 CRISPRa guide guide (pRCA594 backbone) 668
Plasmid#238187PurposeLentiviral CRISPR guide vector expressing a KLF4 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorInsertKLF4 sgRNA CRISPRa (KLF4 Synthetic, Human)
UseCRISPR and LentiviralTagsmTagBFP2ExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Par6-ExRai-CKAR2
Plasmid#236101PurposeSubcellularly targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells for selective detection of aPKC activity within the Par complex.DepositorInsertPar6C-ExRai-CKAR2 (PARD6A Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FLAG-RGS14
Plasmid#236567PurposeExpresses Flag-RGS14 in mammalian cellsDepositorInsertHomo sapiens regulator of G-protein signaling 14 (RGS14 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
SS18_IDR-EGFP
Plasmid#232756PurposeExpresses SS18_IDR-EGFPDepositorInsertSS18_IDR-EGFP (SS18 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCpep-E1GFP
Plasmid#233884PurposeExpression of a pH sensitive variant of EGFP inserted in the C-peptide of insulin for pH measurement in insulin secretory granulesDepositorInsertProinsulin-E1GFP (Ins2 Mouse)
UseTagsExpressionMammalianMutationInserted E1GFP into C-peptide of mouse Ins2 codin…PromoterCMVFAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc W139A
Plasmid#223560PurposeAlias: Gαi bONE-GO W139A. Lentiviral vector expressing GINIP-Nluc W139A after a low efficiency IRES downstream of YFP-CAAX placed after the promotorDepositorInsertYFP(Venus) - KRas4b - IRES* - human GINIP W139A - linker(GGGS) - Nluc
UseLentiviralTagsExpressionMammalianMutationW139A mutation in GINIP sequencePromoterCMVAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET14-H2Kb-ST3
Plasmid#223221PurposeExpression of mouse MHC H2Kb heavy chain fused to Spytag003 under T7 promoter for expression in bacteriaDepositorInsertExpression of soluble mouse MHC H2KB heavy chain (HLA-A Mouse)
UseTagsSpytagExpressionBacterialMutationPromoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-SS-VN155(I152L)-IRE1-dLD
Plasmid#217752PurposeExpresses the VN fragment of BiFC-IRE1-dLDDepositorInsertSerine/threonine-protein kinase/endoribonuclease IRE1 (ERN1 Human)
UseTagsVenus (1-154, I152L)ExpressionMammalianMutationdeleted amino acids 29-408PromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry
Plasmid#231007PurposeProtein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.DepositorInsertE4F1 aa220-242 (E4F1 Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1-NbON10-NanoLuc
Plasmid#198691PurposeExpresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell cultureDepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody ON10
UseTags6xHis tag, IgK leader sequence, NanoLuc, and twin…ExpressionMammalianMutationPromoterCMV promoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1, residues 316-366, C-Cys
Plasmid#191234PurposeBacterial expression for IST1 residues 316-366, contains non-native C-terminal Cysteine for labelingDepositorInsertIST1 (IST1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 316-366, contains nonnative C-terminus f…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP4C 156-233
Plasmid#108257PurposeExpresses C-terminal 156-233 residues of CHMP4C with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-9DepositorInsertCHMP4C residues 156-233 (CHMP4C Human)
UseTagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP5 159-222
Plasmid#108259PurposeExpresses C-terminal 159-222 residues of CHMP5 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-10DepositorInsertCHMP5 residues 148-219 (CHMP5 Human)
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP1A 140-196
Plasmid#104593PurposeExpresses C-terminal 140-196 residues of CHMP1A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-1DepositorInsertCHMP1A residues 140-196 (CHMP1A Human)
UseTagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP2A 152-222
Plasmid#104594PurposeExpresses C-terminal 152-222 residues of CHMP2A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-3DepositorInsertCHMP2A residues 152-222 (CHMP2A Human)
UseTagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP2B 141-213
Plasmid#104595PurposeExpresses C-terminal 141-213 residues of CHMP2B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-4DepositorInsertCHMP2B residues 141-213 (CHMP2B Human)
UseTagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP3 159-222
Plasmid#104599PurposeExpresses C-terminal 159-222 residues of CHMP3 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-5DepositorInsertCHMP3 residues 159-222 (CHMP3 Human)
UseTagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJExpress401-KDAC7 CD
Plasmid#224341PurposeExpresses human KDAC7 (HDAC7) catalytic domain in E. coliDepositorInsertKDAC7 (HDAC7 Human)
UseTagsTEV-cleavable His6ExpressionBacterialMutationOnly includes residues 521-942 (catalytic domain).PromoterT5Available sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJExpress401-KDAC4 CD
Plasmid#224251PurposeExpresses human KDAC4 (HDAC4) catalytic domain in E. coliDepositorInsertKDAC4 (HDAC4 Human)
UseTagsTEV-cleavable His6ExpressionBacterialMutationOnly includes residues 648-1083 (catalytic domain…PromoterT5Available sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJExpress401-KDAC8 H143A
Plasmid#224342PurposeExpresses human KDAC8 (HDAC8) H143A in E. coliDepositorInsertKDAC8 H143A (HDAC8 Human)
UseTagsTEV-cleavable His6ExpressionBacterialMutationHistidine 143 mutated to alaninePromoterT5Available sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBacI-KDAC7 CD
Plasmid#224345PurposeExpresses human KDAC7 (HDAC7) catalytic domain in insect cellsDepositorInsertKDAC7 (HDAC7 Human)
UseTagsTEV-cleavable His6ExpressionInsectMutationOnly includes residues 521-942 (catalytic domain).PromoterPolyhedrinAvailable sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSuper Retro GFP DGK alfa human
Plasmid#224578PurposeTo stably downregulate the expression of human DGK alfa in human cellsDepositorInsertsh RNAi Diacylglycerol kinase alfa human (DGKA Human)
UseRNAi and RetroviralTagsExpressionMutationPromoterH1Available sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT3GEPIR-Rb1shRNA1
Plasmid#220584PurposeTo inducibly knockdown Rb1 expressionDepositorInsertRb1 shRNA1 (RB1 Human)
UseLentiviralTagsExpressionMutationPromoterTRE3G (TetOP) promoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT3GEPIR-Rb1shRNA2
Plasmid#220585PurposeTo inducibly knockdown Rb1 expressionDepositorInsertRb1 shRNA2 (RB1 Human)
UseLentiviralTagsExpressionMutationPromoterTRE3G (TetOP) promoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT3GEPIR-Rb1shRNA3
Plasmid#220586PurposeTo inducibly knockdown Rb1 expressionDepositorInsertRb1 shRNA3 (RB1 Human)
UseLentiviralTagsExpressionMutationPromoterTRE3G (TetOP) promoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRHD-TetR-GAL80-Rho1-GAL4
Plasmid#218274PurposeIntroduction of low-temperature-inducible GAL system, LowTempGAL v2.2DepositorInsertgal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2
UseTagsExpressionYeastMutationDHFR (P66L), Rho1 (C17R)PromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC-C Sars-CoV-2 S_T1027I
Plasmid#194850PurposeSars-CoV-2 S_T1027I in MAC-C destination vectorDepositorInsertS_T1027I (S Sars-CoV-2)
UseTagsMAC-CExpressionMammalianMutationT1027IPromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-C Sars-CoV-2 S_Y144del
Plasmid#194854PurposeSars-CoV-2 S_Y144del in MAC-C destination vectorDepositorInsertS_Y144del (S Sars-CoV-2)
UseTagsMAC-CExpressionMammalianMutationY144delPromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-C Sars-CoV-2 S_E484K
Plasmid#194836PurposeSars-CoV-2 S_E484K in MAC-C destination vectorDepositorInsertS_E484K (S Sars-CoV-2)
UseTagsMAC-CExpressionMammalianMutationE484KPromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-C Sars-CoV-2 S_H655Y
Plasmid#194837PurposeSars-CoV-2 S_H655Y in MAC-C destination vectorDepositorInsertS_H655Y (S Sars-CoV-2)
UseTagsMAC-CExpressionMammalianMutationH655YPromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-C Sars-CoV-2 S_K417N
Plasmid#194838PurposeSars-CoV-2 S_K417N in MAC-C destination vectorDepositorInsertS_K417N (S Sars-CoV-2)
UseTagsMAC-CExpressionMammalianMutationK417NPromoterAvailable sinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits