We narrowed to 7,694 results for: ALP

1. pAAV_Donor/Reporter-COL4A3

   Plasmid

   #130279

   Purpose

   COL4A3 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A3 donor template

   Depositor

   Silvestro Conticello

   Article

   Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

   Insert

   mCherry-eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_COL4A3_self-cleavingCas9

   Plasmid

   #130281

   Purpose

   Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A3 sgRNA targets to induce its self-cleaving and inactivation

   Depositor

   Silvestro Conticello

   Article

   Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

   Insert

   spCas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV_COL4A5_self-cleavingCas9

   Plasmid

   #130282

   Purpose

   Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A5 sgRNA targets to induce its self-cleaving and inactivation

   Depositor

   Silvestro Conticello

   Article

   Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

   Insert

   spCas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRC/CMV-TYK2(delta)TK-VSV

   Plasmid

   #139353

   Purpose

   Expression of TYK2 protein missing the tyrosine kinase (TK) domain (aa 1-895)

   Depositor

   Sandra Pellegrini

   Article

   Velazquez et al J Biol Chem. 1995 Feb 17;270(7):3327-34.

   Insert

   TYK2 cDNA expressing aa 1-895 (TYK2 Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   V362F-deletion of aa 896-1187

   Promoter

   CMV

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. hKCNB1 L211P

   Plasmid

   #124487

   Purpose

   missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

   Depositor

   Federico Sesti

   Article

   Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

   Insert

   human KCNB1 (KCNB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed leucine 211 to proline

   Promoter

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. hKCNB1 R312H

   Plasmid

   #124488

   Purpose

   missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

   Depositor

   Federico Sesti

   Article

   Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

   Insert

   human KCNB1 (KCNB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed arginine 312 to histidine

   Promoter

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. hKCNB1 G381R

   Plasmid

   #124490

   Purpose

   missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

   Depositor

   Federico Sesti

   Article

   Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

   Insert

   human KCNB1 (KCNB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed glycine at 381 to arginine

   Promoter

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. hKCNB1 F416L

   Plasmid

   #124491

   Purpose

   missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

   Depositor

   Federico Sesti

   Article

   Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

   Insert

   human KCNB1 (KCNB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed phenyalanine 416 to leucine

   Promoter

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. hKCNB1 G379R

   Plasmid

   #124489

   Purpose

   missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

   Depositor

   Federico Sesti

   Article

   Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

   Insert

   human KCNB1 (KCNB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed glycine at 379 to arginine

   Promoter

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FB026

    Plasmid

    #119711

    Purpose

    Transcriptional Unit (TU) for YFP expression, obtained by combining FB/GBparts FB007+GB0053+FB008 into pDGB3alpha1R. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    Promoter gpdA:YFP coding sequence:Terminator trpC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSi-check2-hRac2 - mut 3'UTR

    Plasmid

    #97161

    Purpose

    For dual luciferase report assay

    Depositor

    Qing Deng

    Article

    Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

    Insert

    RAC 3'UTR (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    miR-722 Binding Mutant

    Promoter

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PIK3R1 gRNA (BRDN0001146657)

    Plasmid

    #77090

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3R1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PIK3R1 gRNA (BRDN0001145056)

    Plasmid

    #77091

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3R1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PIK3R1 gRNA (BRDN0001148578)

    Plasmid

    #77092

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3R1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PRKCA gRNA (BRDN0001145588)

    Plasmid

    #75926

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKCA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKCA (PRKCA Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RPS6KA2 gRNA (BRDN0001148104)

    Plasmid

    #75733

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA2 (RPS6KA2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENTR-TER+-shDIXDC1-Ms-58

    Plasmid

    #61236

    Purpose

    Entry clone encoding shRNA to mouse DIXDC1

    Depositor

    Reuben Shaw

    Article

    Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

    Insert

    Dixdc1 (Dixdc1 Mouse)
    

    Use

    Entr clone

    Tags

    Expression

    Mutation

    Promoter

    H1/TO

    Available Since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pENTR-TER+-shDIXDC1-Ms-57

    Plasmid

    #61235

    Purpose

    Entry clone encoding shRNA to mouse DIXDC1

    Depositor

    Reuben Shaw

    Article

    Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

    Insert

    Dixdc1 (Dixdc1 Mouse)
    

    Use

    Entr clone

    Tags

    Expression

    Mutation

    Promoter

    H1/TO

    Available Since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

19. C21-bio-His

    Plasmid

    #53405

    Purpose

    Expresses full-length ES1 protein homolog precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    C21 (GATD3 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. HINT2-bio-His

    Plasmid

    #53406

    Purpose

    Expresses full-length Histidine triad nucleotide-binding protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    HINT2 (HINT2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge