Skip to main content
Addgene
Showing: 41 - 80 of 655 results

  5. pGEX-GST-BcPC-PLC

    Plasmid
    #224255
    Purpose
    Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli
    Depositor
    Chiaki Murakami
    Article
    Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:
    Insert
    Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)
    Use
    Tags
    GST and HRV-3C (LEVLFQGP) site
    Expression
    Bacterial
    Mutation
    The gene of Bacillus cereus ATCC 14579 was used f…
    Promoter
    lac promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    224255-plasmid-map-sequence-id-457146

    Enlarge

  10. pMT1004

    Plasmid
    #8613
    Depositor
    Robert Hartley
    Insert
    binase, barstar
    Use
    Tags
    Expression
    Bacterial
    Mutation
    as pMT1002
    Promoter
    Available since
    Jan. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
    8613-plasmid-map-sequence-id-240677

    Enlarge

  11. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

    Plasmid
    #107581
    Purpose
    B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    mCherry
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pdh promoter Bacillus megaterium DSM319
    Available since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    107581-plasmid-map-sequence-id-209155

    Enlarge

  13. pAD-SPS6-Pman

    Plasmid
    #133906
    Purpose
    Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.
    Depositor
    Oscar Kuipers
    Article
    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57
    Insert
    sfGFP(SPS6)
    Use
    E.coli/bacillus spp. shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pman
    Available since
    Nov. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    133906-plasmid-map-sequence-id-261727

    Enlarge

  17. pIA027

    Plasmid
    #200418
    Purpose
    amyE::(dronPA cm), for dronPA fusions. PALM-compatible integration vector for Bacillus subtilis. Includes dronPA photoswitchable FP with multi cloning sites (MCS) for fast and interchangeable cloning.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Tags
    dronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200418-plasmid-map-sequence-id-405455

    Enlarge

  18. pGLU_112

    Plasmid
    #225793
    Purpose
    RS421 library variant 13926
    Depositor
    Nili Ostrov
    Insert
    13926_[Tn5]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225793-plasmid-map-sequence-id-447017

    Enlarge

  19. pGLU_76

    Plasmid
    #225778
    Purpose
    RS421 library variant 13926
    Depositor
    Nili Ostrov
    Insert
    13926_[abr]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225778-plasmid-map-sequence-id-446991

    Enlarge

  20. pGLU_94

    Plasmid
    #225808
    Purpose
    RS421 library variant 13926
    Depositor
    Nili Ostrov
    Insert
    13926_[Himar]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225808-plasmid-map-sequence-id-446976

    Enlarge

  27. pAD-KPS12-Pman

    Plasmid
    #133832
    Purpose
    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.
    Depositor
    Oscar Kuipers
    Article
    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57
    Insert
    mKate(KPS12)
    Use
    E.coli/bacillus spp. shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pman
    Available since
    Nov. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    133832-plasmid-map-sequence-id-261722

    Enlarge

  28. pSPAsp-hp

    Plasmid
    #48122
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):
    Insert
    predicted optimal signal peptide for Bacillus megaterium protein secretion
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    48122-plasmid-map-sequence-id-69949

    Enlarge

  29. pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc

    Plasmid
    #129105
    Purpose
    Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone production
    Depositor
    Eleftherios Terry Papoutsakis
    Article
    Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10
    Insert
    BsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 11, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129105-plasmid-map-sequence-id-255085

    Enlarge

  30. pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107582
    Purpose
    B. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    GFP+
    Use
    Synthetic Biology; E. coli and bacillus shuttle v…
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    fba promoter Bacillus megaterium DSM319
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107582-plasmid-map-sequence-id-209150

    Enlarge

  35. pAIP4_2D5J

    Plasmid
    #234143
    Purpose
    Heterologous protein expression of unsaturated glucuronyl hydrolase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2D5J
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234143-plasmid-map-sequence-id-464109

    Enlarge

  38. pAIP4_1NG6

    Plasmid
    #234191
    Purpose
    Heterologous protein expression of YqeY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1NG6 (yqeY Bacillus subtilis)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234191-plasmid-map-sequence-id-464156

    Enlarge

  39. pAIP4_2OHW

    Plasmid
    #234178
    Purpose
    Heterologous protein expression of YueI in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2OHW (yueI Bacillus subtilis)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234178-plasmid-map-sequence-id-464143

    Enlarge

Showing: 41 - 80 of 655 results