We narrowed to 262 results for: map2

1. MAP2K7 gRNA (BRDN0001162319)

   Plasmid

   #77821

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAP2K7

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAP2K7 (MAP2K7 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MAP2K7 gRNA (BRDN0001149491)

   Plasmid

   #77822

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAP2K7

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAP2K7 (MAP2K7 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MAP2K7 gRNA (BRDN0001162371)

   Plasmid

   #77823

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAP2K7

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAP2K7 (MAP2K7 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MAP2K4 gRNA (BRDN0001148854)

   Plasmid

   #76652

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAP2K4

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAP2K4 (MAP2K4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMSCV-FKBP-MAP2K4

   Plasmid

   #221295

   Purpose

   Expression of MAP2K4 (pLxIS) in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   MAP2K4 (pLxIS) (Map2k4 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG-4xFKBP

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHAGE-MAP2K1-S222A

   Plasmid

   #116429

   Purpose

   Lentiviral expression of MAP2K1 S222A

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S222A

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MAP2K5 gRNA (BRDN0001147345)

   Plasmid

   #77263

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAP2K5

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAP2K5 (MAP2K5 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MAP2K5 gRNA (BRDN0001147402)

   Plasmid

   #77264

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAP2K5

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAP2K5 (MAP2K5 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MAP2K5 gRNA (BRDN0001147422)

   Plasmid

   #77265

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAP2K5

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAP2K5 (MAP2K5 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MAP2K6 gRNA (BRDN0001145878)

    Plasmid

    #76039

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP2K6

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP2K6 (MAP2K6 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MAP2K6 gRNA (BRDN0001145312)

    Plasmid

    #76040

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP2K6

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP2K6 (MAP2K6 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MAP2K6 gRNA (BRDN0001147036)

    Plasmid

    #76041

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP2K6

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP2K6 (MAP2K6 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. R777-E124 Hs.MAP2K2-nostop

    Plasmid

    #70408

    Purpose

    Gateway ORF clone of human MAP2K2 [NM_030662.3] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    MAP2K2 (MAP2K2 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. R777-E122 Hs.MAP2K1-nostop

    Plasmid

    #70406

    Purpose

    Gateway ORF clone of human MAP2K1 [NM_002755.3] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWZL Neo Myr Flag MAP2K5

    Plasmid

    #20514

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    MAP2K5 (MAP2K5 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pWZL Neo Myr Flag MAP2K7

    Plasmid

    #20516

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    MAP2K7 (MAP2K7 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pWZL Neo Myr Flag MAP2K6

    Plasmid

    #20515

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    MAP2K6 (MAP2K6 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pTol1:uas-myc-act-map2k2b-2a-tdTomato

    Plasmid

    #104721

    Purpose

    UAS-driven expression of activated MEK

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    activated map2k2b (map2k2b Zebrafish)
    

    Use

    Zebrafish transposon

    Tags

    2A-tdTomato and myc tag

    Expression

    Mutation

    serines 221 and 225 to aspartic acid

    Promoter

    10xUAS

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5-HA-metap2

    Plasmid

    #100512

    Purpose

    Expresses HA tagged metap2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    Metap2 (METAP2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLJM1-FLAG-metap2

    Plasmid

    #100510

    Purpose

    Expresses FLAG tagged metap2 in mammalian cells to make lentivirus

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    METAP2 (METAP2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLC242-GFP-metap2

    Plasmid

    #100518

    Purpose

    Expresses GFP tagged metap2 in mammalian cells to make lentivirus

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    Metap2 (METAP2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRK5-Myc-metap2

    Plasmid

    #100516

    Purpose

    Expresse Myc tagged metap2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    Metap2 (METAP2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRK5-myc Metap2

    Plasmid

    #199685

    Purpose

    Transient expression of Metap2

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    METAP2 (METAP2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLKO shLuc/FLAG Metap2

    Plasmid

    #209316

    Purpose

    Lentiviral construct expressing FLAG Metap2 and shRNA against Luciferase (control shRNA)

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    METAP2 (METAP2 Human, human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRK5 GST MetaP2

    Plasmid

    #112750

    Purpose

    express HA-GST-FRB-MetaP2

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    HA-GST-FRB-MetaP2 (METAP2 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJAF205

    Plasmid

    #60407

    Purpose

    Flag-tagged full length GMAP210/Trip11

    Depositor

    Gregory Pazour

    Article

    Follit et al PLoS Genet. 2008 Dec;4(12):e1000315. doi

    Insert

    GMAP210 (Trip11 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

27. shMEK1/2 puro

    Plasmid

    #72567

    Purpose

    Hairpin targeting MEK1 and MEK2 (murine).

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    MEK1/2 (Map2k1, Map2k2 Mouse)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. shMEK1/2 v2 puro

    Plasmid

    #72568

    Purpose

    Hairpin targeting MEK1 and MEK2 (murine).

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    MEK1/2 (Map2k1, Map2k2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. shMEK1/2 v2 neo

    Plasmid

    #72570

    Purpose

    Hairpin targeting MEK1 and MEK2 (murine).

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    MEK1/2 (Map2k1, Map2k2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. shMEK1/2 neo

    Plasmid

    #72569

    Purpose

    Hairpin targeting MEK1 and MEK2 (murine).

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    MEK1/2 (Map2k1, Map2k2 Mouse)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDFDuet-1 MKK4(EE)-MKK7a1(EE)

    Plasmid

    #47580

    Purpose

    Bacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Inserts

    MKK4-EE (MAP2K4 Human)
    MKK7a1-EE (Map2k7 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks the first 36 residues of the RefSeq protein…

    Promoter

    Available since

    Sept. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

32. TAL2324

    Plasmid

    #36750

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    MAP2K4-TALEN_Left (Map2k4 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

33. TAL2325

    Plasmid

    #36751

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    MAP2K4-TALEN_Right (Map2k4 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pLenti-Tet-MKK6(DD)-Puro

    Plasmid

    #86094

    Purpose

    Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vector

    Depositor

    Cindy Miranti

    Article

    Frank et al J Cell Sci. 2017 Apr 26. pii: jcs.197152

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Serine 207 and Threonine 211 both changed to Aspa…

    Promoter

    CMV/TetO

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. WT-MEK1-V5

    Plasmid

    #217297

    Purpose

    Lentivirus vector for expression of MEK1

    Depositor

    Channing Der

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MEK1-DD-V5

    Plasmid

    #217298

    Purpose

    Lentivirus vector for expression of MEK1 S218D/S222D

    Depositor

    Channing Der

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    S218D/S222D

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MEK1-K57N-V5

    Plasmid

    #217300

    Purpose

    Lentivirus vector for expression of MEK1 K57N

    Depositor

    Channing Der

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    K57N

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MEK1-Q56P-V5

    Plasmid

    #217299

    Purpose

    Lentivirus vector for expression of MEK1 Q56P

    Depositor

    Channing Der

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Q56P

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MEK1-E102-I103del-V5

    Plasmid

    #217301

    Purpose

    Lentivirus vector for expression of MEK1 E102-I103deletion

    Depositor

    Channing Der

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    E102-I103deletion

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. KW1

    Plasmid

    #21211

    Depositor

    Paul Khavari

    Article

    Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

    Insert

    Mek2 (MAP2K2 Human)
    

    Use

    Subclone vector

    Tags

    Expression

    Mutation

    KW71, constitutive active

    Promoter

    Available since

    July 24, 2009

    Availability

    Academic Institutions and Nonprofits only

41. W1

    Plasmid

    #21208

    Depositor

    Paul Khavari

    Article

    Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

    Insert

    Mek1 (MAP2K1 Human)
    

    Use

    Subclone vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2009

    Availability

    Academic Institutions and Nonprofits only

42. pMCL-HA-MAPKK1-8E (K97M)

    Plasmid

    #40811

    Depositor

    Natalie Ahn

    Article

    Mansour et al Science. 1994 Aug 12;265(5174):966-70.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K97M

    Promoter

    CMV

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

43. pMCL-HA-MAPKK1-WT

    Plasmid

    #40808

    Depositor

    Natalie Ahn

    Article

    Mansour et al Science. 1994 Aug 12;265(5174):966-70.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

44. pMM9-MAPKK2-WT

    Plasmid

    #40805

    Depositor

    Natalie Ahn

    Article

    Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

    Insert

    MAP2K2 (MAP2K2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    MSV-LTR

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

45. pMCL-HA-MAPKK2-KW71[delta(48-55)/S222D/S226D]

    Plasmid

    #40821

    Depositor

    Natalie Ahn

    Article

    Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

    Insert

    MAP2K2 (MAP2K2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta(48-55) + S222D + S226D

    Promoter

    CMV

    Available since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

46. pMCL-HA-MAPKK1-11A55B [S218E/S222D]

    Plasmid

    #40809

    Depositor

    Natalie Ahn

    Article

    Mansour et al Science. 1994 Aug 12;265(5174):966-70.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S218E, S222D

    Promoter

    CMV

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

47. pNESx2-EGFP-C1-PABDx2-Spo20

    Plasmid

    #220082

    Purpose

    PA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear export

    Depositor

    Gerry Hammond

    Article

    Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

    Insert

    Spo20 (SPO20 Budding Yeast)
    

    Use

    Tags

    X. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…

    Expression

    Mammalian

    Mutation

    amino acids 51-91, tandem dimer

    Promoter

    CMV

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pGEX-4T-1 3xHA-MEK1-DD

    Plasmid

    #47576

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MEK1-DD (Map2k1 Mouse)
    

    Use

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Ser218 to Asp, Ser221 to Asp

    Promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

49. pMCL-HA-MAPKK1-R4F [delta(31-51)/S218E/S222D]

    Plasmid

    #40810

    Depositor

    Natalie Ahn

    Article

    Mansour et al Science. 1994 Aug 12;265(5174):966-70.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta(31-51), S218E, S222D

    Promoter

    CMV

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

50. pcDNA3-MEK5DD-HA

    Plasmid

    #65247

    Purpose

    Expression of HA tagged dominant active MEK5 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

    Insert

    MEK5DD (MAP2K5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Ser311 to Asp, Ser315 to Asp

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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