Skip to main content
Addgene
Showing: 21 - 40 of 74 results
  1. pBJ7

    Plasmid
    #167136
    Purpose
    A tetracyclin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter
    Depositor
    Insert
    luxCDABE
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Kanamycin promoter
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pALS3-Ma-SUMO-TAG35-sfGFP

    Plasmid
    #212120
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Inserts
    SUMO-TAG35-sfGFP
    M. alvus Pyl-tRNA
    Use
    Tags
    His6 and SUMO-TAG35
    Expression
    Bacterial
    Mutation
    E35TAG (within SUMO encoding sequence)
    Promoter
    araC and lpp
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. mUAVbla:tetR 5′

    Plasmid
    #149379
    Purpose
    mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs
    Depositor
    Insert
    tetA
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pREVTRE-AID

    Plasmid
    #121067
    Purpose
    Retrovirus vector expresses AID-fusion protein under TRE promoter and with hygromycin resistance selectio
    Depositor
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    AID
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pAGE2.0

    Plasmid
    #165984
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Tet resistance.
    Depositor
    Inserts
    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    tetracycline resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pBGE2.0

    Plasmid
    #165987
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Tet resistance.
    Depositor
    Inserts
    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    tetracycline resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pENTR-THT

    Plasmid
    #55790
    Purpose
    GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Kanamycin resistance
    Depositor
    Type
    Empty backbone
    Use
    RNAi; Gateway
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    modified H1 RNA gene
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
  8. pENTR-THT III

    Plasmid
    #55791
    Purpose
    GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Gentamycin resistance
    Depositor
    Type
    Empty backbone
    Use
    RNAi; Gateway
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    modified H1 RNA gene
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
  9. pXGFPC-5 Pxyl-dcas9 (Sth3)

    Plasmid
    #133317
    Purpose
    expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Insert
    dcas9 (Streptococcus thermophilus #3)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    xylose
    Available since
    Jan. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pDEINO3

    Plasmid
    #179487
    Purpose
    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation
    Depositor
    Insert
    TetR/A
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. pVCERC-5 Pvan-dcas9 (Sth3)

    Plasmid
    #133319
    Purpose
    expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Insert
    dcas9 (Streptococcus thermophilus #3)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    vanillate
    Available since
    Jan. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. pXGFPC-5 Pxyl-dcas9 (Spa)

    Plasmid
    #133318
    Purpose
    expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Insert
    dcas9 (Streptococcus pasteurianus)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    xylose
    Available since
    Feb. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
  13. pXGFPC-5 Pxyl-dcas9 (Spy)

    Plasmid
    #133316
    Purpose
    expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Insert
    dcas9 (Streptococcus pyogenes)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    xylose
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
  14. pWJB9

    Plasmid
    #126650
    Purpose
    Expresses AdrA from a modified pLAFR3
    Depositor
    Insert
    adrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
    Use
    Tags
    8xHis
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
  15. pAH25-SceI

    Plasmid
    #129389
    Purpose
    Derived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.
    Depositor
    Insert
    Chloramphenicol acetyltransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N/A
    Promoter
    CAT promoter
    Available since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 21 - 40 of 74 results