Addgene

We narrowed to 11,705 results for: cel.2

Showing: 381 - 400 of 11705 results

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GFP-Bcl-2-T132L

Plasmid

#220303

Purpose

mammalian expression of Bcl-2-T132L tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Use

Tags

Emerald GFP

Expression

Mammalian

Mutation

T132L

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-A131L

Plasmid

#220302

Purpose

mammalian expression of Bcl-2-A131L tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Use

Tags

Emerald GFP

Expression

Mammalian

Mutation

A131L

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-G128L

Plasmid

#220301

Purpose

mammalian expression of Bcl-2-G128L tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Use

Tags

Emerald GFP

Expression

Mammalian

Mutation

G128L

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-ΔBH4

Plasmid

#220300

Purpose

mammalian expression of Bcl-2-ΔBH4 tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Use

Tags

Emerald GFP

Expression

Mammalian

Mutation

BH4 deleted

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pVC299 VEGF Site#2

Plasmid

#47506

Purpose

human gRNA expression vector targeting VEGF

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-VEGF site 2 (VEGFA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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shMEK1/2 v2 neo

Plasmid

#72570

Purpose

Hairpin targeting MEK1 and MEK2 (murine).

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

MEK1/2 (Map2k1, Map2k2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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shMEK1/2 v2 puro

Plasmid

#72568

Purpose

Hairpin targeting MEK1 and MEK2 (murine).

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

MEK1/2 (Map2k1, Map2k2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL2-2

Plasmid

#109009

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-sgRNA #2 (ATL2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TREK-2(S156C)

Plasmid

#224770

Purpose

In vitro transcription for Oocyte expression of 1 TREK-2(S156C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 10 (Kcnk10 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S156C

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pX330A-Yap1-E1-#2

Plasmid

#171519

Purpose

deletion of a genomic locus in Yap1 gene

Depositor

Hans Schöler

Article

Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

Insert

Yap1 (Yap1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-HisA-HOXC6-2

Plasmid

#110365

Purpose

Mammalian expression of HisA-tagged HOXC6-2

Depositor

Carlos Moreno

Article

Ramachandran et al Oncogene. 2005 Jan 6;24(1):188-98. doi:

Insert

HOXC6 (HOXC6 Human)

Use

Tags

HIS

Expression

Mammalian

Mutation

short isoform

Promoter

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL1-2

Plasmid

#109006

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL1-sgRNA #2 (ATL1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-muscle Amphiphysin 2

Plasmid

#22213

Depositor

Pietro De Camilli

Article

Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

Insert

Amphiphysin II (BIN1 Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP-IMP2-2

Plasmid

#42175

Depositor

Alexandra Kiemer

Article

Kessler et al Am J Physiol Gastrointest Liver Physiol.

Insert

Insulin-like growth factor 2 mRNA binding protein 2-2 (IGF2BP2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

T7

Available since

June 14, 2013

Availability

Academic Institutions and Nonprofits only
lentiCRISPR v2-sgBAP1-2

Plasmid

#125838

Purpose

KO BAP1 gene

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - EGFP sgRNA 2

Plasmid

#51761

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

Feng Zhang

Article

Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

Inserts

Cas9
Puromycin resistance
EGFP sgRNA 2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS and U6

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiCriprV2-sgRNA-PER1-#2

Plasmid

#189988

Purpose

Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Period1 (PER1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBIG1b_nsp7 (SARS-CoV-2)

Plasmid

#169178

Purpose

Baculoviral transfer vector to express untagged nsp7 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp7 (ORF1ab SARS-CoV-2, Synthetic)

Use

Tags

untagged

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1c_nsp8 (SARS-CoV-2)

Plasmid

#169179

Purpose

Baculoviral transfer vector to express untagged nsp8 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp8 (ORF1ab SARS-CoV-2, Synthetic)

Use

Tags

untagged

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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px335 Mettl14 sgRNA #2

Plasmid

#61514

Purpose

encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

Depositor

Jacob Hanna

Article

Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

Insert

gccgctcccggatctcctgc

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only