We narrowed to 22,109 results for: ESC

1. pGEM-mcpGFP

   Plasmid

   #47442

   Purpose

   contains modified circularly permutated EGFP (mcpGFP)

   Depositor

   Wolf Frommer

   Article

   De Michele et al eLife. 2013 Jul 2;2:e00800.

   Insert

   mcpGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   M154K, V164A, S176G, D181Y, T204V, A207K, V190I

   Promoter

   Available since

   Sept. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pDOC-K-glmS-lacIZ

   Plasmid

   #158061

   Purpose

   Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome

   Depositor

   Mark Webber

   Article

   Holden et al Microbiology (Reading). 2020 Nov 23. doi

   Insert

   lacIZ operon
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFA6a-link-yomApple-CaURA3

   Plasmid

   #44879

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mApple with CaUra3 selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomApple
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTH849-gst-optec

   Plasmid

   #89590

   Purpose

   Plasmid for bacterial expression of His-tagged GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase codon-optimised for bacterial expression
   

   Use

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC19-P70a-Top-T~P70a-Bottom-T

   Plasmid

   #87314

   Purpose

   Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli system
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P70a

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p3s-eCas9-1.1

   Plasmid

   #104172

   Purpose

   eCas9-1.1 mammalian expression

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

   Insert

   eCas9-1.1
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   CMV

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. POM42-mTFP1

   Plasmid

   #69852

   Purpose

   N-terminal tagging with mTFP1 using URA3 as a selection marker in S. cerevisiae.

   Depositor

   Liza Pon

   Article

   Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

   Insert

   mTFP1 minus start and stop codon
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRSETB-ro1GFP

   Plasmid

   #82365

   Purpose

   Inducible expression of redox sensitive GFP in bacteria

   Depositor

   S. James Remington

   Article

   Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

   Insert

   ro1GFP
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C48S/Q80R/S147C/Q204C

   Promoter

   T7

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG373-mito-redFAST

   Plasmid

   #160358

   Purpose

   Expresses mito-redFAST in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

   Insert

   mito-redFAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. VAChT-SNAP

    Plasmid

    #105318

    Purpose

    VAChT tagged with SNAP

    Depositor

    Justin Taraska

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    VAChT (Slc18a3 Rat)
    

    Use

    Tags

    SNAP tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-PRLSS-SH2-KDEL

    Plasmid

    #209716

    Purpose

    Mammalian expression of SH2 localized to endoplasmic reticulum

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    PRLSS-SH2-KDEL
    

    Use

    Tags

    PRLSS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFA6a-link-yomKO2-CaURA3

    Plasmid

    #44881

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mKO2 with CaUra3 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomKO2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET30a-YedK

    Plasmid

    #86884

    Purpose

    Bacterial expression of E. coli SRAP ortholog YedK

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    YedK (yedK Eschericia coli)
    

    Use

    Tags

    6xHis, HA tag, and S-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEB1-Clover

    Plasmid

    #103991

    Purpose

    Plasmid encoding Clover

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    Clover
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S30R, Y39N, …

    Promoter

    proC

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNH082

    Plasmid

    #42165

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    mTFP1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET29b_Qcat18

    Plasmid

    #45146

    Depositor

    Christopher Petzold

    Article

    Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

    Insert

    E-coli-pathways-QconCAT18
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LI A-B EF1 c2

    Plasmid

    #54435

    Depositor

    Martin Parniske

    Article

    Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

    Insert

    promoter region of A. thaliana EF1alpha1 /AT1G07940.1
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMUT2-pAto-mCherry

    Plasmid

    #192858

    Purpose

    pMUT2 based vector for sensing acetoacetate in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    Acetoacetate inducible promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

    Plasmid

    #175522

    Purpose

    Nuclear expression of HaloTag11 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBbE1k-2

    Plasmid

    #59945

    Purpose

    dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

    Depositor

    Taek Soon Lee

    Article

    Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

    Inserts

    dihydropterine reductase
    pterin-4a-carbinolamine dehydratase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pTrc

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Kohinoor-Paxillin/pcDNA3

    Plasmid

    #67775

    Purpose

    Paxilin-targetted Kohinoor for mammalian cell expression

    Depositor

    Takeharu Nagai

    Article

    Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

    Insert

    Kohinoor
    

    Use

    Tags

    Paxillin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pSA10(gfcD)

    Plasmid

    #176191

    Purpose

    gfcD protein expression

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcD
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSA10(gfcD-10xHis)

    Plasmid

    #176192

    Purpose

    gfcD protein expression

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcD
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSA10(gfcBCD)

    Plasmid

    #176193

    Purpose

    expresses 3 overlapping genes (gfcB, gfcC, gfcD) from this one operon

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcBCD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mCerulean3-Paxillin-22

    Plasmid

    #55436

    Purpose

    Localization: Focal Adhesions, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Paxillin
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pSB_init-Sb66

    Plasmid

    #159422

    Purpose

    Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb66

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    Sb66
    

    Use

    Tags

    c-Myc and poly-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mCerulean3-PMP-N-10

    Plasmid

    #55439

    Purpose

    Localization: Peroxisomes, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    PMP (PXMP2 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pGK415-­rtetTAK8N, L131L

    Plasmid

    #165969

    Purpose

    Expresses rTetTA (K8N, L131L) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    tetR
    

    Use

    Synthetic Biology

    Tags

    three copies of VP16 (VP48)

    Expression

    Yeast

    Mutation

    Promoter

    pPGK1

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pActinin-TagRFP675-N1

    Plasmid

    #44278

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

    Insert

    TagRFP675
    

    Use

    Tags

    Actinin

    Expression

    Mammalian

    Mutation

    M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

    Promoter

    CMV

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TDGS50

    Plasmid

    #183189

    Purpose

    TEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoter

    Depositor

    Hsiao-Chun Huang

    Article

    Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

    Inserts

    DivIVA-GFP(tevR M0050)
    TEV-S219V
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLBC0030

    Plasmid

    #172777

    Purpose

    Level 0 module containing NT2_nYFP

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    CDS N-terminal eYFP (1-174)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLBC0031

    Plasmid

    #172778

    Purpose

    Level 0 module containing CT_nYFP

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    CDS N-terminal eYFP (1-174)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLBC0040

    Plasmid

    #172779

    Purpose

    Level 0 module containing NT2_cYFP

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    CDS C-terminal eYFP (175-238)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLBC0041

    Plasmid

    #172780

    Purpose

    Level 0 module containing CT_cYFP

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    CDS C-terminal eYFP (175-238)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pETM6_Pfrm_BsMdh_BmHps_BmPhi

    Plasmid

    #129109

    Purpose

    Expresses Bacillus subtilis Mdh and Bacillus methanolicus Hps & Phi under Pfrm promoter

    Depositor

    Eleftherios Terry Papoutsakis

    Article

    Rohlhill et al ACS Synth Biol. 2017 Aug 18;6(8):1584-15

    Insert

    BsMDH BmHPS BmPHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pEB1-Venus SX

    Plasmid

    #103990

    Purpose

    Plasmid encoding Venus SX

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    Venus SX
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F46L, F64L, …

    Promoter

    proC

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEB1-mVenus JBC

    Plasmid

    #103987

    Purpose

    Plasmid encoding mVenus JBC

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mVenus JBC
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F46L, F64L, …

    Promoter

    proC

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Lifeact-rsLOV2 pcDNA3.1(+)

    Plasmid

    #107882

    Purpose

    Mammalian expression of Lifeact-rsLOV2

    Depositor

    Stefan Hell

    Article

    Gregor et al Sci Rep. 2018 Feb 9;8(1):2724. doi: 10.1

    Insert

    rsLOV2
    

    Use

    Tags

    Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p15aC-4D1D

    Plasmid

    #107864

    Purpose

    Bacterial expression of PIS and PI4K

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Inserts

    PIS
    phosphatidylinositol 4-kinase β
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    proD

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJAT13

    Plasmid

    #18986

    Depositor

    Jay Keasling

    Article

    Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

    Insert

    promoter CP13
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 8, 2008

    Availability

    Academic Institutions and Nonprofits only