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pTn7-SCOUT14.G

Plasmid

#201002

Purpose

pLv1-pUC18R6KT-miniTn7-aad9-sfGFP, SpR, StR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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3XFlag-pLPC

Plasmid

#73560

Purpose

vector encoding 3 Flag tags

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

3X-Flag

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA-D1

Plasmid

#37480

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Insert

D1 second generation cameleon (calcium sensor)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
pGEM-mcpGFP

Plasmid

#47442

Purpose

contains modified circularly permutated EGFP (mcpGFP)

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

mcpGFP

Expression

Bacterial

Mutation

M154K, V164A, S176G, D181Y, T204V, A207K, V190I

Available Since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-emiRFP703

Plasmid

#197205

Purpose

Expresses the protein of emiRFP703 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

emiRFP703

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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Gag-delta-p6-mEGFP

Plasmid

#116890

Purpose

expresses Gag missing the p6 domain, tagged with mEGFP, in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

synthetic Gag missing the p6 domain

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b_PfSSB

Plasmid

#103000

Purpose

The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB

Depositor

Martin Webb

Article

Chisty et al PLoS One. 2018 Feb 21;13(2):e0193272. do

Insert

PfSSB

Expression

Bacterial

Promoter

T7

Available Since

May 24, 2018

Availability

Academic Institutions and Nonprofits only

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p3s-Cas9-HF1

Plasmid

#104173

Purpose

Cas9-HF1 mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

Cas9-HF1

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomApple-SpHis5

Plasmid

#44844

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mApple with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomApple

Expression

Yeast

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCDF-EcAvs2

Plasmid

#188804

Purpose

Codon optimized EcAvs2 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs2

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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P4U2C8T2-v1-1 (Pl-tetO1-deCFP) (135p)

Plasmid

#70211

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deCFP

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomKO2-CaURA3

Plasmid

#44881

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKO2 with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKO2

Expression

Yeast

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_GFP*_Intein_DD

Plasmid

#162812

Purpose

STELLA plasmid for amber suppression of C-terminally labelled GFP

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

GFP(C-TAG)-Intein-DD

Use

Piggybac

Expression

Mammalian

Mutation

GFP(Opal247,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pNH082

Plasmid

#42165

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Expression

Worm

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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p3s-eCas9-1.1

Plasmid

#104172

Purpose

eCas9-1.1 mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

eCas9-1.1

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoLSS-mKate2-CaURA3

Plasmid

#44886

Purpose

Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoLSS-mKate2

Expression

Yeast

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pNB783

Plasmid

#60162

Purpose

Firefly luciferase (Promega) driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

A4V & S504G (no functional effect)

Promoter

RNR1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pEB2-Katushka-9-5

Plasmid

#104011

Purpose

Plasmid encoding Katushka-9-5

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Katushka9-5

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (K6S, N21D, R32G, L…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pQFT-lacZ

Plasmid

#208896

Purpose

pQFT with lacZ under control of PQJ

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Insert

lacZ

Expression

Bacterial

Mutation

Contains two silent mutations and a missense muta…

Promoter

PQJ

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRSETB-ro1GFP

Plasmid

#82365

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro1GFP

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/Q80R/S147C/Q204C

Promoter

T7

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb2E7

Plasmid

#184681

Purpose

NIR-Fb to HIV gp41

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb2E7

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBCC155

Plasmid

#74995

Purpose

CFP-based BiFC vector for transient expression of CC (155-238) in plants

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

CFP (155–239)/CC155

Expression

Plant

Promoter

CaMV35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBCN155

Plasmid

#74994

Purpose

CFP-based BiFC vector for transient expression of CN (1-155) in plants

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

CFP (1–155)/CN155

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD009

Plasmid

#116850

Purpose

Expresses araC constitutively and alpha-hemolysin under a pBAD promoter; alternate name pSB1A2-AD009

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
alpha-HL

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pKB239

Plasmid

#170034

Purpose

Encodes LacI-controlled expression of surface-displayed GNLY and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
Granulysin (GNLY Synthetic, Human, Escherichia coli)

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Paxillin/pcDNA3

Plasmid

#67775

Purpose

Paxilin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

Paxillin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
pJC20cdc8-cerulean3

Plasmid

#99358

Purpose

Bacterial expression of Cerulean3 fused to the 3' of cDNA of the fission yeast tropomyosin, cdc8.

Depositor

Dan Mulvihill

Article

Brooker et al FEBS Lett. 2016 Sep;590(18):3111-21. doi

Insert

cdc8 (cdc8 Fission Yeast)

Tags

Cerulean3 and Gly3-His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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DmU6 3p3 GFP LgRNA

Plasmid

#182207

Purpose

For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Drosophila

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

3XP3-GFP

Use

CRISPR

Expression

Insect

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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POM42-mTFP1

Plasmid

#69852

Purpose

N-terminal tagging with mTFP1 using URA3 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1 minus start and stop codon

Expression

Yeast

Available Since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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mCerulean3-PMP-N-10

Plasmid

#55439

Purpose

Localization: Peroxisomes, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

PMP (PXMP2 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
mCerulean3-Paxillin-22

Plasmid

#55436

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Paxillin

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pBAD-iGECInano

Plasmid

#186189

Purpose

Expression of small FRET-based near-infrared genetically-encoded calcium indicator in bacteria

Depositor

Vladislav Verkhusha

Article

Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

Insert

iGECInano

Expression

Bacterial

Promoter

AraC

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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VAChT-SNAP

Plasmid

#105318

Purpose

VAChT tagged with SNAP

Depositor

Justin Taraska

Article

Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

Insert

VAChT (Slc18a3 Rat)

Tags

SNAP tag

Expression

Mammalian

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomApple-CaURA3

Plasmid

#44879

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mApple with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomApple

Expression

Yeast

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pTH849-gst-optec

Plasmid

#89590

Purpose

Plasmid for bacterial expression of His-tagged GST encoded by preferred codons

Depositor

Tobias von der Haar

Article

Jossé et al bioRxiv 200006

Insert

glutathione-S transferase codon-optimised for bacterial expression

Tags

8xhistidine

Expression

Bacterial

Promoter

T7

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pActinin-TagRFP675-N1

Plasmid

#44278

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

Insert

TagRFP675

Tags

Actinin

Expression

Mammalian

Mutation

M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

Promoter

CMV

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat18

Plasmid

#45146

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT18

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4xh7SKPylT_EF1_NoBody*_Intein_DD

Plasmid

#162813

Purpose

STELLA plasmid for amber suppression of C-terminally labelled NoBody

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

NoBody(C-TAG)-Intein-DD

Use

Piggybac

Expression

Mammalian

Mutation

NoBody(Opal69,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yoClover-SpHis5

Plasmid

#44857

Purpose

Yeast tagging vector to create gene fusion with yeast optimized Clover with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoClover

Expression

Yeast

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pDOC-K-glmS-lacIZ

Plasmid

#158061

Purpose

Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome

Depositor

Mark Webber

Article

Holden et al Microbiology (Reading). 2020 Nov 23. doi

Insert

lacIZ operon

Use

Synthetic Biology

Expression

Bacterial

Promoter

lac

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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