We narrowed to 17,912 results for: REV

1. pET28 basic.3

   Plasmid

   #191881

   Purpose

   Encodes a GFP design, basic.3

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   basic.3
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   T65S V68A S72A Y145S L220V V224I

   Promoter

   T7

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28 mid.2

   Plasmid

   #191880

   Purpose

   Encodes a GFP design, mid.2

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   mid.2
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   T108I Y145I

   Promoter

   T7

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28 bleach.6

   Plasmid

   #191879

   Purpose

   Encodes a GFP design, bleach.6

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   bleach.6
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   V61L S72C Y145M H181L V224I

   Promoter

   T7

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28 slow.3

   Plasmid

   #191877

   Purpose

   Encodes a GFP design, slow.3

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   slow.3
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Q69L S72V Y145S T167V L220V V224I

   Promoter

   T7

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28 basic.2

   Plasmid

   #191911

   Purpose

   Encodes a GFP design, basic.2

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   basic.2
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   S72A V112I Y145V L220V V224I

   Promoter

   T7

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPgiCas13b

   Plasmid

   #184833

   Purpose

   Inducible expression of PgiCas13b nuclease in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Cas13b nuclease from Porphyromonas gingivalis
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. DpbdCas12e VPR

   Plasmid

   #188515

   Purpose

   Expresses FLAG tagged DpbdCas12e VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12e
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D672A/E769A/D935A

   Promoter

   EF1a

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKLV2-EF1a-ArgSen (-)P2A (-)NLS

   Plasmid

   #184690

   Purpose

   Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   ArgSen (-)P2A (-)NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV-EF1a-ArgSen (-)NLS

   Plasmid

   #184698

   Purpose

   Lentiviral transfer plasmid for expression of ArgSen (-)NLS

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   ArgSen (-)NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV-EF1a-ArgSen (-)P2A (-)NLS

    Plasmid

    #184699

    Purpose

    Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    ArgSen (-)P2A (-)NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYI006

    Plasmid

    #170840

    Purpose

    FRO6p::GUSPlus::tOCS in pFP100

    Depositor

    Christian Fankhauser

    Article

    Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

    Insert

    FRO6p::GUSPlus::tOCS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    FRO6

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYI046

    Plasmid

    #170842

    Purpose

    (pUBQ10::SMT2-FLAG::tOCS)

    Depositor

    Christian Fankhauser

    Article

    Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

    Insert

    pUBQ10::SMT2-FLAG::tOCS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pUBQ10

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAcrVIB1-cloDF-kan

    Plasmid

    #184836

    Purpose

    Expression of anti-CRISPR protein AcrVIB1 in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    anti-CRISPR protein inhibiting Cas13b nucleases
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pfa6a mScarlet-FKBP::KANMX

    Plasmid

    #185905

    Purpose

    Plasmid for cloning mScarlet fused to FKBP into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    mScarlet-FKBP
    

    Use

    Tags

    mScarlet fused to FKBP

    Expression

    Yeast

    Mutation

    Promoter

    SP6

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMM4_14

    Plasmid

    #183062

    Purpose

    Plasmid expressing a biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under RET2p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al Microb Cell Fact. 2022 Oct 15;21(1):214.

    Inserts

    mCherry
    BM3R1-HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    HREsYGP1p and RET2

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pILGFP4D41

    Plasmid

    #185841

    Purpose

    Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJCble

    Plasmid

    #185861

    Purpose

    The CRISPR/Cas9-and-gRNA-expressing plasmid with a phleomycin-resistent selectable marker

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; ble
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pILGFP10CF72 (B11)

    Plasmid

    #185859

    Purpose

    Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pILGFP8EFA

    Plasmid

    #185854

    Purpose

    Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    DHFR P66L

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 2Bc-T MBP_BoMoC(ed)_W403A_6xH

    Plasmid

    #185712

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed W403 to A

    Promoter

    T7

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. KBB430

    Plasmid

    #185091

    Purpose

    NMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    First nuclear localization signal mutated (amino …

    Promoter

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. KBB428

    Plasmid

    #185090

    Purpose

    NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    First nuclear localization signal mutated (amino …

    Promoter

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pfa6a mScarlet-TsCC(B)::KANMX

    Plasmid

    #176397

    Purpose

    Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    mScarlet-TsCC(B)
    

    Use

    Tags

    mScarlet fused to TsCC(B)

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    none

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pX459-HypaCas9-CfANLN_sgRNA

    Plasmid

    #183880

    Purpose

    pX459V2.0-HypaCas9 plasmid with cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    Canis familiaris ANLN sgRNA spacer
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWZL hygro-hTCAB1

    Plasmid

    #182593

    Purpose

    expresses hTCAB1 in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    hTCAB1 (WRAP53 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGB-Co-NF-kB

    Plasmid

    #180206

    Purpose

    Expression GAL4-fusion of Co-NF-kB in yeast

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    Co-NF-kB
    

    Use

    Tags

    GAL4 (1-147) fusion

    Expression

    Yeast

    Mutation

    aa 2-1224

    Promoter

    AdhI

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMT-EGFP-CTAP-PrP-SBP_W

    Plasmid

    #147835

    Purpose

    Insect Expression of EGFP

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV-hGFAP-ND1-K139D-T2A-mCherry

    Plasmid

    #178587

    Purpose

    Expression of NEUROD1-K139D and mCherry under the human GFAP promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    NEUROD1-K139D (NEUROD1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    NEUROD1-K139D

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAV-hGFAP-ND1-R111L-T2A-mCherry

    Plasmid

    #178586

    Purpose

    Expression of NEUROD1-R111L and mCherry under the human GFAP promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    NEUROD1-R111L (NEUROD1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    NEUROD1-R111L

    Promoter

    Available since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMC3-TPK2

    Plasmid

    #176180

    Purpose

    yeast MoClo level-0 part plasmid type 3 containing TPK2 gene

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    TPK2 (TPK2 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLV Pigb-V5

    Plasmid

    #175160

    Purpose

    Lentiviral expression of mouse Pigb-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Pigb (Pigb Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHAGE-eGFP-TAX1BP1 632-639D

    Plasmid

    #175771

    Purpose

    Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 632-639D

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    632-639D

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHAGE-eGFP-OPTN D474N

    Plasmid

    #175762

    Purpose

    Lentiviral vector expressing N-terminally tagged eGFP-OPTN D474N

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D474N

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pOME0135

    Plasmid

    #178397

    Purpose

    KSHV Kaposincluster-RevCompl-Syn

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

    Insert

    Kaposincluster-RevCompl-SYN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAT15488_HeF-ABE8e

    Plasmid

    #174126

    Purpose

    plasmid expressing HeF-ABE8e

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HeF-ABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAT15487_evo-ABE8e

    Plasmid

    #174125

    Purpose

    plasmid expressing evo-ABE8e

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    evo-ABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAT15486_HypaR661A-ABE8e

    Plasmid

    #174124

    Purpose

    plasmid expressing HypaR661A-ABE8e

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HypaR-ABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAT15484_HF-ABE8e

    Plasmid

    #174122

    Purpose

    Expresses ABE8e fused with SpCas9-HF1

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HF-ABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. NmMetQ

    Plasmid

    #172111

    Purpose

    Expression of Methionine Binding Protein (MetQ) with signal sequence

    Depositor

    Douglas Rees

    Article

    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

    Insert

    Methionine Binding Protein
    

    Use

    Tags

    10x Histidine Tag and N.meningitidis signal seque…

    Expression

    Bacterial

    Mutation

    Changed Proline 22 to Alanine

    Promoter

    T7 Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Sc NybbR eRF3∆165N (H348E)

    Plasmid

    #175420

    Purpose

    Encodes His-MBP-TEV-ybbR-H348E eRF3∆165N

    Depositor

    Joseph Puglisi

    Article

    Lawson et al Science. 2021 Aug 20;373(6557):876-882.

    Insert

    eRF3 (SUP35 Budding Yeast)
    

    Use

    Tags

    His-MBP-TEV and ybbR

    Expression

    Bacterial

    Mutation

    ∆165N, H348E

    Promoter

    T7

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAT9994-HypaR661A-ABE

    Plasmid

    #163002

    Purpose

    plasmid expressing HypaR661A-ABE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    Hypa-ABE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    R661A

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAT9996-HeF-ABE

    Plasmid

    #163004

    Purpose

    plasmid expressing HeF-ABE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HeF-ABE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA3.1-mas-PRGRH-Nluc

    Plasmid

    #158608

    Purpose

    Plasmid expressing PRG(RH)-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - PDZ RhoGEF RH domain - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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44. pcDNA3.1-mas-p115RH-Nluc

    Plasmid

    #158607

    Purpose

    Plasmid expressing p115(RH)-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - p115RhoGEF RH domain - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pET28a(+)-HisTim12_CtoA

    Plasmid

    #170281

    Purpose

    Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

    Insert

    yeast Tim12 (TIM12 Budding Yeast)
    

    Use

    Tags

    TEV-cleavable N-terminal His tag (on backbone)

    Expression

    Bacterial

    Mutation

    mutated two non-conserved Cys to Ala

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. Ash1

    Plasmid

    #169711

    Purpose

    Bacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)

    Depositor

    Tanja Mittag

    Article

    Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323

    Insert

    Ash1 (ASH1 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Truncation including encoding amino acids 420-500

    Promoter

    T7

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCDNA3_ReBphP-PCM_IRES_sfGFP

    Plasmid

    #166917

    Purpose

    Express ReBphP-PCM and sfGFP

    Depositor

    Andre Stiel

    Article

    Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

    Inserts

    ReBphP-PCM
    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pRS406 ATG1-ATG7-3'UTR

    Plasmid

    #166847

    Purpose

    Expresses Atg1 with ATG7 3'UTR in yeasts.

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

    Insert

    ATG1 (ATG1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ATG1 Promoter

    Available since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pRS405 VAC8-G2A,C4A,C5A,C7A-RFP

    Plasmid

    #166842

    Purpose

    Expresses a myristoylation and palmitoylation mutant Vac8-RFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7Ala

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8 Budding Yeast)
    

    Use

    Tags

    RFP

    Expression

    Yeast

    Mutation

    G2A, C4A, C5A, C7A

    Promoter

    VAC8 promoter

    Available since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pRS405 VAC8-G2A,C4A,C5A,C7A-GFP

    Plasmid

    #166839

    Purpose

    Expresses a myristoylation and palmitoylation mutant Vac8-GFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7Ala

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    G2A, C4A, C5A, C7A

    Promoter

    VAC8 promoter

    Available since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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